H2O2(Hydrogen Peroxide) Mycelium Syringe

Home | Community | Message Board

You are not signed in.
New Account

Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop:

Spores for European Microscopy

Liquid Cultures

Bulk Substrate

All-In-One Bags That Don't Suck

Bulk Cannabis Seeds

Jump to first unread post

Pages: 1 | 2 | Next > [ show all ]

Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.

Search59
Yet anotherhobby for me.

Registered: 10/28/04
Posts: 116

H2O2(Hydrogen Peroxide) Mycelium Syringe

#3430868 - 12/01/04 12:29 AM (21 years, 10 months ago)

Here is a H2O2 mycelium syringe using ideas from the petri peroxide agar tek. After searching I found nothing mentioning the amount of peroxide to use in the myc water or how to go about it.
Heres first steps with pics.

1. Supplies:
-Get 500ml of distilled and boiled water(cooled down in container with sealed lid)(tap water has chlorine which kills myc and destroys H2O2)
-Get a bottle of H2O2 (3% from store)
-Get a syringe (with a screw on/off removable needle)
-Get a shot glass
-Boil some water
-A flame
-Plastic Gloves
-A flat-head blade(xacto kits have blades that are flat on the end, not pointed)
-Peroxide Agar Petri with good mycelium growth

2-Sterilize your syringe Needle, Flame it! Sterilize your syringe, suck up boiling water into it, and let sit for 5 minutes, squeeze all water out into sink. Then suck H2O2(3%) into syringe (suck as much as you can) and let sit for 5 minutes. Squeeze out into sink.

3-Sterilize your shot glass, pour boiling water into it, over fill it, and let it sit for 5 minutes. Dump into sink. Then after, fill it with straight H2O2(3%) and let sit for 5 minutes) Dump into sink.

4-Suck 3ML of H2O2(3%) into syringe and inject all into 500ml of water. Stir around with needle a bit.

5-Suck up a FULL syringe of the (500ml)Water+(3ml)H2O2 into the needle. Naturally you can't get it all, 500ml was used to keep the numbers even and not fractional.

6-Open Peroxide Agar Petri and take sterile flathead blade and lightly scrape off the top of the agar to remove mycelium ONLY. It will look as if your removing almost nothing at all. DON'T DIG into the agar at all, just scrape the mycelium off the top of it, it will happen very eaisly.

7-Hold flat blade over shot glass, and take needle(with the H2O2+water) and wash the flat blade with myc on it into shot glass. Repeat this for more mycelium in your shot glass water. When done, squeeze all water+h2o2 out of syringe into shot glass.

8-FILL syringe lid with H2O2(3%) and put the lid back on the empty syringe. (filling it isn't easy, tap lid while filling it) UNSCREW syringe needle and set needle aside.

9-Take needle-less syringe and put into shot glass full of mycelium+H2O2+water and quickly suck ALL of shot glass contents into syringe.

10-Screw syringe needle back on.

(if you find your mycelium is too thick to inject with needle on, when sucking up into needle-less syringe, plunge it back out quickly into shot glass to break up mycelium do this a few times if needed, you can then put needle on and plunge it out again into shot glass, and suck it up with needle on to break it up even more)

Use this syringe immediately, as its prolly NOT a good way to store mycelium. H2O2 breaks down under light. I don't know, I have never tried this to store long term Myc syringes. I also don't know so far if this method is going to be too "dirty" and introduce contams. If so, then I might suggest adding even more H2O2 to the 500ml of water.

--------------------
The only beneficiaries of Prohibition were bootleggers(drug makers), crime bosses(drug pushers), and the forces of big government(DEA).
(wheres the benefit for the consumer?)

You gotta check this out.
http://www.di.fm/mp3/chillout96k.pls

Ride the wave of the mellow fractal rainbow!

Edited by Search59 (12/01/04 01:15 AM)

Extras:

Similar Threads	Poster	Views	Replies	Last post
H2O2 - Hydrogen Peroxide	SumGuy	2,289	4	08/30/01 03:06 PM by BINDEGAL
Hydrogen Peroxide questions...	djcontra	3,433	13	06/05/01 03:02 PM by pl151
Hydrogen Peroxide in the Humidity?	igore	5,953	2	04/23/03 10:45 AM by Diploid
Hydrogen Peroxide (h2o2) often propagated mistruths	lysergic	7,245	11	07/13/03 04:45 PM by Baby_Hitler
Hydrogen Peroxide, will it kill mycelia?	sillvyr	8,010	8	01/29/02 02:51 PM by sillvyr
hydrogen peroxide?	Yaniphc	2,365	6	11/09/01 05:38 PM by Yaniphc
Hydrogen Peroxide / Water solution wetting casing material ?	JohnnyRespect	3,086	2	04/18/03 10:53 PM by RogerRabbit
Hydrogen peroxide (h2o2) in perlite	parsonsproject	3,131	1	11/27/01 12:08 AM by dimitri211