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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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InvisibleTM
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Cloning Question
    #714263 - 06/30/02 07:57 PM (21 years, 2 months ago)

I am attempting to clone PC (Cambodian). Using malt agar in 3 petri dishes innoculated with clean inner stem flesh and being kept at 88 degrees, the question is; How long before I should notice mycelial growth?

If the answer to this is in a FAQ, I couldn't find it.
If the answer to this is not finite, please give an estimate.

Finally, if this question does not belong here, I apologize in advance.

Thank you.


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"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

TM™ :cool:


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InvisibleChampion des Champignons
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Registered: 07/26/00
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Re: Cloning Question [Re: TM]
    #714764 - 06/30/02 11:59 PM (21 years, 2 months ago)

a week at the most, probably less..


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hmmm........


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OfflineXhulan
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Re: Cloning Question [Re: Champion des Champignons]
    #714870 - 07/01/02 02:19 AM (21 years, 2 months ago)

Usually its very fast, taking around 72hours, but it can go up to a week depending on a lot of factors.


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Offlinezeronio
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Re: Cloning Question [Re: Xhulan]
    #714878 - 07/01/02 02:28 AM (21 years, 2 months ago)

One factor can be peroxide. It takes longer if you use it.


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InvisibleChampion des Champignons
long standing member;)

Registered: 07/26/00
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Re: Cloning Question [Re: zeronio]
    #715013 - 07/01/02 04:44 AM (21 years, 2 months ago)

oops
true, true, I always use peroxidated agar, so don't know any better


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hmmm........


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Offlinetchyted
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Registered: 09/03/01
Posts: 526
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Re: Cloning Question [Re: TM]
    #715096 - 07/01/02 05:35 AM (21 years, 2 months ago)

i don't know about your particular strain, and i clone in licuid form, or directly onto substrate, not agar. in licuid, five days is typical, direct method, you will see hyphal fuzz within 24 to 48 hours, which should become obviously rizomorphic within five days typical. i always use peroxide.


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InvisibleTM
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Re: Cloning Question [Re: Champion des Champignons]
    #715171 - 07/01/02 06:46 AM (21 years, 2 months ago)

No peroxide in my malt agar, so I should see some fuzz any minute now I guess, unless there's something wrong...

Thanks guys! You're the best!


--------------------
================================================



"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

TM™ :cool:


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InvisibleTM
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Folding@home Statistics
Registered: 06/11/02
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YeeHaa! [Re: Champion des Champignons]
    #717256 - 07/02/02 03:48 AM (21 years, 2 months ago)

All 3 dishes are showing nice healthy growth!! 5 days!


--------------------
================================================



"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

TM™ :cool:


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Invisiblemeatman
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Re: YeeHaa! [Re: TM]
    #720288 - 07/03/02 11:44 AM (21 years, 2 months ago)

congrats! i hope everything turns out well for you. i'd like to see some pics if you've got a camera. if not, i just wanna know how it goes. g'luck


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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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