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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Open [Re: DivinePsychosis]
    #7063145 -

DivinePsychosis said:
I don't know why so many people on here are afraid of taping their jars before pc'ing.I've always used electrical tape to seal the holes before I pc the jars and it does the trick with surprisingly little mess and no foil is required.

When it comes time to inoculate the jars I just lift the tape up and shoot the spore water into the jar,then press the tape back down.After the jars are colonized a slight bit I take the tape off and put a thin layer of tissue paper over the holes.The tissue paper allows plenty of FAE and provides a bit of extra protection against contams.


I guess everyone has their own methods and logic. Your idea seems pretty good. I was under the impression that tape AND foil was just extra precaution to keep moisture out when cooking. I personally never understood why you couldn't cook it without holes, then sterilize a nail and poke the holes after the cook.


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Everything I say is either hypothetical or a lie. Assume that nothing I say is true, except what I am saying right now.

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Re: Open [Re: freemynd]
    #7063181 -

freemynd:

I suppose you could do it that way too,although after you use the jars once and are ready to use them for a second batch you'd have to come up with a method for sealing the holes anyway.

I know some people on another board who don't even bother with holes,they just lift the lid and quickly shoot the solution into the jar.Apparently by their logic microscopic moldspores have plenty of room to seep into the jars when opening the innoc holes to shoot the spore solution than when lifting the lid and quickly shooting around.

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Re: Open [Re: DivinePsychosis]
    #7063219 -

DivinePsychosis said:
I don't know why so many people on here are afraid of taping their jars before pc'ing.I've always used electrical tape to seal the holes before I pc the jars and it does the trick with surprisingly little mess and no foil is required.

When it comes time to inoculate the jars I just lift the tape up and shoot the spore water into the jar,then press the tape back down.After the jars are colonized a slight bit I take the tape off and put a thin layer of tissue paper over the holes.The tissue paper allows plenty of FAE and provides a bit of extra protection against contams.

I'm telling you electrical tape works damn good!It's very easy to take off come inoculation time and it seals the jars so well that a small whirlwind appears in the jars when lifting it off.


Taping is fine IF YOU REMOVE IT AT THE PROPER TIME! Many many people don't for years we have had this problem! There is absolutely no reason what so ever to put the tape back on the lids after inoculation to do so is a flawed technique simply redundant and thats a fact no experienced cultivator can argue. Now tissue paper that I can say without a doubt is a bad idea and will set you up for failure many more times than not. Good luck with that you will need it :wink: Also  with the PFTek your verm layer is your only needed contam barrier.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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Re: Open [Re: hyphae]
    #7063261 -

Hey hyphea.

Why exactly is tissue paper a bad idea?I've had no problems with it and once I remove the tape and apply it the jars seem to speed up.I would imagine a problem could arise from mold particles saturating in the tissue and dropping into the jars but like I said I havn't had a problem with it so far.It seems clean and fresh for me.

PS:I had to edit the whirlwind bit,it sounded a little overplayed.It's more like a drift of wind not a damn natural disaster lol.Just lift the tape slowly so it doesn't mix the two layers ya know?

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Re: Open [Re: freemynd]
    #7063269 -

freemynd said:
I personally never understood why you couldn't cook it without holes, then sterilize a nail and poke the holes after the cook.


It is not proper sterile culture technique. It's is like putting the cart before the horse, You want in this hobby to have your bases covered at all times. Are you going to use a sterile nail and hammer or drill in a glovebox or in front of a flowhood to make your holes? KISS is the rule around here.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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Re: Open [Re: DivinePsychosis]
    #7063282 -

DivinePsychosis said:
Hey hyphea.

Why exactly is tissue paper a bad idea?I've had no problems with it and once I remove the tape and apply it the jars seem to speed up.I would imagine a problem could arise from mold particles saturating in the tissue and dropping into the jars but like I said I havn't had a problem with it so far.It seems clean and fresh for me.

PS:I had to edit the whirlwind bit,it sounded a little overplayed.It's more like a drift of wind not a damn natural disaster lol.Just lift the tape slowly so it doesn't mix the two layers ya know?


To answer it simply tissue absorbs moisture and molds easily you've been lucky is all. Again GL


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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Re: Open [Re: hyphae]
    #7092449 -

I think my friend might have messed up. Basically he taped his holes, covered with foil and sterilized. After the cook, he removed the foil and inoculated through the tape (poked through the tape). Then he removed the tape all together. I think during the process he touched a couple of the holes (after inoculation). They are now sitting on the shelf without any tape. Does anyone know if this will be a problem?


--------------------
Everything I say is either hypothetical or a lie. Assume that nothing I say is true, except what I am saying right now.

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Re: Open [Re: freemynd]
    #7097876 -

sorry to keep digging up an old thread...but here's a quick question. if so much time is spent sterilizing the needle, the jars, and the glovebox, why then, would it not matter if the holes are left uncovered? does it just need to be sterile during that moment when inoculation happens? otherwise, leaving the holes open would leave the jars open to contaminations and whatnot (especially if one is just leaving the jars sitting on a shelf). is this right?


--------------------
Everything I say is either hypothetical or a lie. Assume that nothing I say is true, except what I am saying right now.

Edited by freemynd (06/27/07 07:14 AM)

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Re: Open [Re: freemynd]
    #7097899 -

Thats what I thought, but apparently they need air and the dry verm layer is a sufficient contam barrier


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KNOW YOUR SOURCE, KNOW YOUR SUBSTANCE, KNOW YOUR LIMIT

ALL MUSHROOMS ARE EDIBLE, BUT SOME ONLY ONCE.

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