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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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to seal the holes or not to...
    #7061372 -

Anyone have faster jar growth with opened or sealed innoc holes? or is there any difference


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KNOW YOUR SOURCE, KNOW YOUR SUBSTANCE, KNOW YOUR LIMIT

ALL MUSHROOMS ARE EDIBLE, BUT SOME ONLY ONCE.

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Re: to seal the holes or not to... [Re: Mephisto616]
    #7061388 -

open, for sure.


--------------------
Do you dare to feel responsible for every dollar you lay down
are you going to make the rich man richer
or are you going to stand your ground
You say you want a revolution
a communal evolution
to be a part of the solution
maybe I'll be seeing you around.

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Re: to seal the holes or not to... [Re: Mephisto616]
    #7061391 -

Mephisto616 said:
Anyone have faster jar growth with opened or sealed innoc holes? or is there any difference


Leave them open. Magash did a 90-jar experiment with 45 sealed and 45 open -- all 45 open finished before any of the sealed. These days, everyone with experience will recommend open.


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I'm interested in Kombucha cultivation!  Send me a kombucha baby, and I'll send you the cost of shipping.  I promise to give away at least 5 babies for every baby I receive this way. :smile:

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Re: to seal the holes or not to... [Re: fish42]
    #7061395 -

i dunno what tek you are using, but just cover them with bandaids, or tyvek if you are using grain jars, and leave them uncovered if you are using BRF with dry verm filter layer. Either way, don't plug them completely, or you will lose all FAE.

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Re: to seal the holes or not to... [Re: GoodbyeOrb]
    #7061421 -

Using PF tek with BRF/Verm in 1/2 pint jars. sealed holes during boiling procedure. going to remove tape for innoc and remain opened for colonization then, thanks guys.


--------------------
KNOW YOUR SOURCE, KNOW YOUR SUBSTANCE, KNOW YOUR LIMIT

ALL MUSHROOMS ARE EDIBLE, BUT SOME ONLY ONCE.

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Re: to seal the holes or not to... [Re: Mephisto616]
    #7061466 -

ick. next time, just cover with tin foil held on with a band. I'd imagine you are going to have fun getting the tape off after it's been to 212 degrees. Either way, just leave the holes uncovered, if you have the dry verm layer.

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Re: to seal the holes or not to... [Re: GoodbyeOrb]
    #7061536 -

tape was easy nuff, have some special tape its packing tape I think. stays together really well. yeah I have the dry Verm layers, thanks Ill leave them uncovered then, also I heard that if you start to see slowing down in the growth, turning them upside down to drop all the heavier CO2 will allow more oxygen to enter and then promote growth at normal speed again, any truth to this?


--------------------
KNOW YOUR SOURCE, KNOW YOUR SUBSTANCE, KNOW YOUR LIMIT

ALL MUSHROOMS ARE EDIBLE, BUT SOME ONLY ONCE.

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Re: to seal the holes or not to... [Re: Mephisto616]
    #7061543 -

Eh, i've heard that same thing, but I haven't done enough PF jars to notice a difference. Theoretically it should work, but you'd have to wait for someone that has tested the theory to give you a definite response. Either way, I don't see how it could hurt, just don't shake your dry verm layer out through your holes!

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Open [Re: GoodbyeOrb]
    #7061633 -


http://youtube.com/profile_videos?user=ShroomCity

It is not necessary to seal the holes. One should not touch the lid of the jars at all.

If one glues sticky tape or other silly stuff over the holes, one is forced to fumble around on the lid after the jars have been sterilized and that is not what we want. Isn't it?

I suggest to use a separate syringe for each jar. It has also the advantage , that one does not need to burn the needle red-hot after each inoculation.

You may look here to get an impression of what I mean:
http://youtube.com/watch?v=wAEb5_s_Yr8

Good grow


--------------------

http://youtube.com/profile_videos?user=ShroomCity
http://audiostreet.net/radelbaluvar
Shrooms Rock and so does Pinky.

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Re: Open [Re: RadelBaluvar]
    #7061668 -

^^ You might want to delete that, as you aren't a sponsor ^^

Mephisto: the point of the tin foil is to keep condensation from getting to the dry vermiculite layer during sterilization.

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Re: Open [Re: GoodbyeOrb]
    #7061694 -

Quote:
^^ You might want to delete that, as you aren't a sponsor ^^



Jackass has been polluting the boards with that all day today. I appreciate the videos, but RR's are better, comprehensive, and this isn't fucking myspace champ. Tone down the banner, especially at the TOP of every post.

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Re: Open [Re: CaptainLinger]
    #7061708 -

I'm surprised an admin/mod hasn't nabbed him.

Anno

There, that should bring some attention to it. :thumbup:

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Re: Open [Re: GoodbyeOrb]
    #7061946 -

So let me get this straight:

If following MM's video, poke 4 holes, cover with tape, sterilize. Then take tape off for inoculation and leave off for the 3-4 weeks?


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Everything I say is either hypothetical or a lie. Assume that nothing I say is true, except what I am saying right now.

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Re: Open [Re: freemynd]
    #7061953 -

yea basically,also take off the alum foil too after innoc


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"Anything i say is fictional"
  what you should look for in manure

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Re: Open [Re: xaxphaanes]
    #7061981 -

xaxphaanes said:
yea basically,also take off the alum foil too after innoc


If I'm not mistaken, if following RR's video, there's no foil being used.


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Everything I say is either hypothetical or a lie. Assume that nothing I say is true, except what I am saying right now.

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Re: Open [Re: freemynd]
    #7062006 -

http://youtube.com/watch?v=ZHJQrsZFQdE

at about 6.50 mins they talk about alum foil on pf tek style jars when you boil the jars


--------------------
"Anything i say is fictional"
  what you should look for in manure

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Re: Open [Re: xaxphaanes]
    #7062546 -

Foil lids during PCing do not use tape thats the updated improved revision of the PFTek in my book.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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Re: Open [Re: hyphae]
    #7062669 -

hyphae said:
Foil lids during PCing do not use tape thats the updated improved revision of the PFTek in my book.


Ok...so according to RR's video he says to turn the lids upside down and put medical tape over the holes, cover with foil, and cook. got it.


--------------------
Everything I say is either hypothetical or a lie. Assume that nothing I say is true, except what I am saying right now.

Edited by freemynd (06/18/07 07:50 PM)

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Re: Open [Re: freemynd]
    #7063081 -

freemynd said:
hyphae said:
Foil lids during PCing do not use tape thats the updated improved revision of the PFTek in my book.


Ok...so according to RR's video he says to turn the lids upside down and put medical tape over the holes, cover with foil, and cook. got it.


Yep works flawlessly! Microtape that is nothing else.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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Re: Open [Re: hyphae]
    #7063135 -

I don't know why so many people on here are afraid of taping their jars before pc'ing.I've always used electrical tape to seal the holes before I pc the jars and it does the trick with surprisingly little mess and no foil is required.

When it comes time to inoculate the jars I just lift the tape up and shoot the spore water into the jar,then press the tape back down.After the jars are colonized a slight bit I take the tape off and put a thin layer of tissue paper over the holes.The tissue paper allows plenty of FAE and provides a bit of extra protection against contams.

I'm telling you electrical tape works damn good!It's very easy to take off come inoculation time and it seals the jars so well that a small whirling of air pressure appears in the jars when lifting it off.

Edited by DivinePsychosis (06/18/07 08:36 PM)

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Re: Open [Re: DivinePsychosis]
    #7063145 -

DivinePsychosis said:
I don't know why so many people on here are afraid of taping their jars before pc'ing.I've always used electrical tape to seal the holes before I pc the jars and it does the trick with surprisingly little mess and no foil is required.

When it comes time to inoculate the jars I just lift the tape up and shoot the spore water into the jar,then press the tape back down.After the jars are colonized a slight bit I take the tape off and put a thin layer of tissue paper over the holes.The tissue paper allows plenty of FAE and provides a bit of extra protection against contams.


I guess everyone has their own methods and logic. Your idea seems pretty good. I was under the impression that tape AND foil was just extra precaution to keep moisture out when cooking. I personally never understood why you couldn't cook it without holes, then sterilize a nail and poke the holes after the cook.


--------------------
Everything I say is either hypothetical or a lie. Assume that nothing I say is true, except what I am saying right now.

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Re: Open [Re: freemynd]
    #7063181 -

freemynd:

I suppose you could do it that way too,although after you use the jars once and are ready to use them for a second batch you'd have to come up with a method for sealing the holes anyway.

I know some people on another board who don't even bother with holes,they just lift the lid and quickly shoot the solution into the jar.Apparently by their logic microscopic moldspores have plenty of room to seep into the jars when opening the innoc holes to shoot the spore solution than when lifting the lid and quickly shooting around.

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Re: Open [Re: DivinePsychosis]
    #7063219 -

DivinePsychosis said:
I don't know why so many people on here are afraid of taping their jars before pc'ing.I've always used electrical tape to seal the holes before I pc the jars and it does the trick with surprisingly little mess and no foil is required.

When it comes time to inoculate the jars I just lift the tape up and shoot the spore water into the jar,then press the tape back down.After the jars are colonized a slight bit I take the tape off and put a thin layer of tissue paper over the holes.The tissue paper allows plenty of FAE and provides a bit of extra protection against contams.

I'm telling you electrical tape works damn good!It's very easy to take off come inoculation time and it seals the jars so well that a small whirlwind appears in the jars when lifting it off.


Taping is fine IF YOU REMOVE IT AT THE PROPER TIME! Many many people don't for years we have had this problem! There is absolutely no reason what so ever to put the tape back on the lids after inoculation to do so is a flawed technique simply redundant and thats a fact no experienced cultivator can argue. Now tissue paper that I can say without a doubt is a bad idea and will set you up for failure many more times than not. Good luck with that you will need it :wink: Also  with the PFTek your verm layer is your only needed contam barrier.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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Re: Open [Re: hyphae]
    #7063261 -

Hey hyphea.

Why exactly is tissue paper a bad idea?I've had no problems with it and once I remove the tape and apply it the jars seem to speed up.I would imagine a problem could arise from mold particles saturating in the tissue and dropping into the jars but like I said I havn't had a problem with it so far.It seems clean and fresh for me.

PS:I had to edit the whirlwind bit,it sounded a little overplayed.It's more like a drift of wind not a damn natural disaster lol.Just lift the tape slowly so it doesn't mix the two layers ya know?

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Re: Open [Re: freemynd]
    #7063269 -

freemynd said:
I personally never understood why you couldn't cook it without holes, then sterilize a nail and poke the holes after the cook.


It is not proper sterile culture technique. It's is like putting the cart before the horse, You want in this hobby to have your bases covered at all times. Are you going to use a sterile nail and hammer or drill in a glovebox or in front of a flowhood to make your holes? KISS is the rule around here.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

Extras: Filter Print Post Top
Re: Open [Re: DivinePsychosis]
    #7063282 -

DivinePsychosis said:
Hey hyphea.

Why exactly is tissue paper a bad idea?I've had no problems with it and once I remove the tape and apply it the jars seem to speed up.I would imagine a problem could arise from mold particles saturating in the tissue and dropping into the jars but like I said I havn't had a problem with it so far.It seems clean and fresh for me.

PS:I had to edit the whirlwind bit,it sounded a little overplayed.It's more like a drift of wind not a damn natural disaster lol.Just lift the tape slowly so it doesn't mix the two layers ya know?


To answer it simply tissue absorbs moisture and molds easily you've been lucky is all. Again GL


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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Re: Open [Re: hyphae]
    #7092449 -

I think my friend might have messed up. Basically he taped his holes, covered with foil and sterilized. After the cook, he removed the foil and inoculated through the tape (poked through the tape). Then he removed the tape all together. I think during the process he touched a couple of the holes (after inoculation). They are now sitting on the shelf without any tape. Does anyone know if this will be a problem?


--------------------
Everything I say is either hypothetical or a lie. Assume that nothing I say is true, except what I am saying right now.

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Re: Open [Re: freemynd]
    #7097876 -

sorry to keep digging up an old thread...but here's a quick question. if so much time is spent sterilizing the needle, the jars, and the glovebox, why then, would it not matter if the holes are left uncovered? does it just need to be sterile during that moment when inoculation happens? otherwise, leaving the holes open would leave the jars open to contaminations and whatnot (especially if one is just leaving the jars sitting on a shelf). is this right?


--------------------
Everything I say is either hypothetical or a lie. Assume that nothing I say is true, except what I am saying right now.

Edited by freemynd (06/27/07 07:14 AM)

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Re: Open [Re: freemynd]
    #7097899 -

Thats what I thought, but apparently they need air and the dry verm layer is a sufficient contam barrier


--------------------
KNOW YOUR SOURCE, KNOW YOUR SUBSTANCE, KNOW YOUR LIMIT

ALL MUSHROOMS ARE EDIBLE, BUT SOME ONLY ONCE.

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