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open air inoculation sucess!
    #7000205 -

I got extremely lucky with innoculating my LC and fungi rye bag in open air. They are both progressing without contamination. However, the bag is progressing at a glacial speed. The LC is almost done colonizing. When its done should I suck up some of that mycelium and inject it into my fungi bag to speed it up?

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Re: open air inoculation sucess! [Re: sHrOoMaCiDe]
    #7000219 -

? i always open air inoculate. I like "glacial" that's great, i've found that with spore to grain before too. LC to grain is much much faster. I don't know anything about adding LC to it to speed it up though.

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Re: open air inoculation sucess! [Re: jeetered]
    #7000259 -

Yeah, open air inoculation isn't really that risky. When I get screwed up, it's usually the substrate conditions that do it.


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FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS
FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS
FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS

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Re: open air inoculation sucess! [Re: figgusfiddus]
    #7000365 -

figgusfiddus said:
Yeah, open air inoculation isn't really that risky. When I get screwed up, it's usually the substrate conditions that do it.



1 cubic foot of normal household air contains on average:
100,000 invisible particulates.
So, every square inch of that air carries:
One hell of a load of possible contaminate spores.

Best success rate for open air inoculation.
Is to continuously keep the needle covered.
With an alc saturated gauze pad, foam, paper towel, or cotton balls.
Otherwise, you will not BE LUCKY, FOR LONG.


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Re: open air inoculation sucess! [Re: agar]
    #7000443 -

agar said:
figgusfiddus said:
Yeah, open air inoculation isn't really that risky. When I get screwed up, it's usually the substrate conditions that do it.



1 cubic foot of normal household air contains on average:
100,000 invisible particulates.
So, every square inch of that air carries:
One hell of a load of possible contaminate spores.

Best success rate for open air inoculation.
Is to continuously keep the needle covered.
With an alc saturated gauze pad, foam, paper towel, or cotton balls.
Otherwise, you will not BE LUCKY, FOR LONG.



YUP:thumbup:

I do all open air and have no problem with contams . I make my lids just for this purpose . I have one 1/4" hole in the lid for GE , and another 1/4" hole with polyfil in it that I soak with alcohol and inoculate through .



I also keep the syringe covered with an alcohol soaked coet .

Peace,.Love,Happiness and Harmony
:heart: Hippie Chick :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058

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Re: open air inoculation sucess! [Re: HippieChick]
    #7000606 -

right on


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"Your mama's grow was so contaminated, the shroomery got trich." :headbanger:
-SpitballJediS

"your a female, no one woulda cared you were naked,hell probably made someone's day, but I pull my balls out on a bus and im the bad guy.."
-Bishlap

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Re: open air inoculation sucess! [Re: sHrOoMaCiDe]
    #7000761 -

do most people here actually use a glovebox or something? SWIM has never had contams as a result of air...the one they got was because of a bad syringe.

lysol for surfaces, oust for the air, dust mask and rubber gloves.

Edited by myndreach (06/02/07 07:30 PM)

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