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OfflinePanTrop
enthusiast
Registered: 11/03/99
Posts: 288
Loc: Washington
Last seen: 20 years, 7 months
Re: do i have to introduce the spores along the glass
    #68801 - 12/17/99 02:02 PM (22 years, 9 months ago)

No you dont have to....thats just the way PF does it.....its actually better to inoculate the center of the cake.

------------------
"My mind dont work if my spine dont jerk"
-Quote from Eminem-

~PanTrop~



--------------------
" My mind dont work if my spine dont jerk!"


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OfflineAnnoA
Experimenter
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Registered: 06/18/99
Posts: 24,148
Loc: my room
Last seen: 1 month, 23 days
Re: do i have to introduce the spores along the glass [Re: PanTrop]
    #68803 - 12/17/99 05:24 PM (22 years, 9 months ago)

My experience is that it?s much better to innoculate along the glas. If you do so, then the water droplet will slide all the way down the glass and inoculate the substrate at many places -> you get much faster and more uniform growth.

------------------
It?s easy to do without silver and gold
or even pleasures of love
but its hard to do without mushrooms.

Marcus Valerius Martialis
Roman poet



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Anonymous

Re: do i have to introduce the spores along the glass [Re: PanTrop]
    #68804 - 12/17/99 08:20 PM (22 years, 9 months ago)

I always inoculate in the center of the cake and it always colonizes perfectly. When I do it the PF four site tek, the bottom of my jars take forever to finish and then you have to wait a couple days for the center to finish. This way the spores germinate in the center and grow out.


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OfflinePsilowarrior
enthusiast
Registered: 11/21/99
Posts: 195
Last seen: 21 years, 3 months
Re: do i have to introduce the spores along the glass [Re: PanTrop]
    #68805 - 12/20/99 12:42 AM (22 years, 9 months ago)

One advantage to innoculating along the glass is that contamination introduced by the syringe can often be seen very quickly.


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Anonymous

Re: do i have to introduce the spores along the glass [Re: PanTrop]
    #68806 - 12/20/99 12:52 AM (22 years, 9 months ago)

It doesn't really matter, there can be benefits to side, and center inoculation.

Basically if you want to see whats going on inoculate the sides (also the water will drip down the side inoculating all along it).

If you don't care and would prefer even growth go for the center. Or do sides and center and be k-rad elite.



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