Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

MagicBag Grow Bags
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co Certified Organic All-In-One Grow Bags   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
clone?
    #6376494 -

when you clone a shroom into a lc, are you isolating that certain strain?

Extras: Filter Print Post Top
Re: clone? [Re: drmyc69]
    #6376570 -

I think your actually starting an isolate for a sub strain.
The strain you got going if from a vendor is just an isolation that grows in whatever geographical location with the exception of some novelty strains that are in fact either hybrids or sub strains.

As you repeat the process from the best specimens you grow from the clones you'll eventually have a genitally superior sub strain.

Extras: Filter Print Post Top
Re: clone? [Re: MajorDick]
    #6376613 -

Here is cream corns tek on how to do it, very noob friendly has a lot of great tips too. http://www.shroomery.org/forums/showflat.php?Cat=0&Board=Forum2&Number=5874305&Searchpage=1&Main=5874305&Words=cloning+easy+tek&topic=&Search=true#Post5874305


--------------------
http://palebluedot.ytmnd.com/ watch this shrooming and youll never think the same about the world agian.

Extras: Filter Print Post Top
Re: clone? [Re: Fingerfoods]
    #6376736 -

do you have to go through the whole process of isolating that sub strain everytime you start a grow or is thier a way to keep that strain going?

Extras: Filter Print Post Top
Re: clone? [Re: drmyc69]
    #6376750 -

One of those LC's will stay good in the fridge for months, and by doing a G2G transfer a few times you can make that LC last a very long time.


--------------------
http://palebluedot.ytmnd.com/ watch this shrooming and youll never think the same about the world agian.

Extras: Filter Print Post Top
Re: clone? [Re: drmyc69]
    #6376754 -

I think what you want to do is make a master LC from the clone and start new LC from that and use them for jars.

Extras: Filter Print Post Top
Re: clone? [Re: JJJJJJ]
    #6379830 -

why do you need to keep your cultures 2nd generation? what would happen if you kept making your LC from the one before?

Extras: Filter Print Post Top
Re: clone? [Re: drmyc69]
    #6380009 -

Senescence
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
Re: clone? [Re: RogerRabbit]
    #6380573 -

oh, does generation mean a full cycle of growth? like making a clone LC(2nd gen.)then inoculating another substrate and fruiting it to get fruits which would be 3rd gen.? right?

Extras: Filter Print Post Top
Re: clone? [Re: drmyc69]
    #6380821 -

'generation' really isn't an applicable term for mycology. Senescence is related to the number of cell divisions regardless of whether the mycelium has entered a fruiting cycle or not. After a couple of months of growth, a mycelial network will slow down considerably, then shortly afterward, the substrate becomes a prime site for contamination because the mycelium can no longer hold its own.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
Re: clone? [Re: RogerRabbit]
    #6405761 -

RR, you continually amaze me.

I take it to mean that making new LC from a Master LC isn't quite right. Kinda like putting a hinge on a pump handle.

The question then has to be, is there any way to prevent senescence other than starting with fresh spores once in awhile? and how many times is it ok to make LC from LC before it becomes and issue?

I have several jars that were inoculated with LC made from a LC that was made from spores. All together it's about 7 months old if you don't restart the timer for making new LC from old LC. Other jars from the same batch inoculated with SS are 98% done, LC is about 50% done. Could this be the cause? And should i keep the jars?

Thanks
JJJJJJ

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co Certified Organic All-In-One Grow Bags   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Shiitake cloning LeopardMan 5,099 18 03/01/10 07:54 AM
by kurshiukas
* Helping hamloaf Understand Senescence.
( 1 2 all )		 hamloafM 2,336 21 04/08/11 05:39 PM
by SKrink
* Will cloning to GLC then to LC unnecessarily induce cell division/senescence? abltsandwich 315 1 01/19/11 02:25 PM
by k00laid
* differences betwen strain isolation by transfers and by cloning?
( 1 2 3 4 all )		 FGL 15,805 62 10/06/09 08:13 PM
by feelfunny
* Multi Spore Grain to Grain & Senescence Crabmeat 6,052 3 07/12/08 08:51 PM
by Bubba McMushies
* Agaricus Bisporus Senescence and Sterility (Infertility?) DragonChaser 2,357 13 02/03/08 12:47 PM
by DragonChaser
* Is senescence subject to fruitbodies? fordrew 955 3 10/27/09 06:27 PM
by Doc_T
* re-cloning? question about senescense Fungal growth 819 5 10/28/10 11:41 AM
by Fungal growth

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
476 topic views. 15 members, 3,394 guests and 123 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.014 seconds on 16 queries.