Would leaving a LC non-inoculated for 24 hours before PCing be helpful?

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Alprazolom
Misfit

Registered: 09/14/06
Posts: 159
Loc: Manchester
Last seen: 19 years, 7 months

Would leaving a LC non-inoculated for 24 hours before PCing be helpful?

#6121396 - 10/01/06 04:13 PM (20 years, 1 month ago)

With rye you soak it for 24 hours so the endospores germinate, making them killable with a PC afterwards. Can the same be done for a LC?

For example can I add honey and water to a jar w/ the polyfill filter and aluminium foil ready to be PCd, but not PC it for 24 hours to let the endospores germinate, and then PC it for 15-20 mins before inoculation?

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My first grow. Thanks all.

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creamcorn
mad scientist

Registered: 03/13/06
Posts: 2,962

Re: Would leaving a LC non-inoculated for 24 hours before PCing be helpful?

[Re: Alprazolom]
#6121408 - 10/01/06 04:19 PM (20 years, 1 month ago)

well it won't hurt

"problem" is that there really aren't going to be endospores in an LC. they're in grain because of the types of bacteria that feed on grain, the length of time they're there, etc...

an LC, especially a honey one is a different situation - honey is actually sterile by nature... and if you're using distilled water (as you should with LC IMO) that's pretty close to sterile if not sterile as well. the only thing that ends up in your LC would be a small bit of airborne contaminates while you're preparing it... again unlikely those would be bacterial endospores.

to top it off, liquid is much easier to sterilize than grains, heat transfers through water so well that its not difficult to get good complete sterilization already.

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