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soma_seeker
Stranger in aStrange Land


Registered: 04/02/06
Posts: 367
Loc: Down under
Last seen: 3 months, 20 days
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repeated contamination
#5759564 - 06/17/06 01:26 AM (17 years, 7 months ago) |
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after 5 or 6 seperate attempts using 3 different LC's 95% of my BRF cakes have had varying amounts of the following contam visible after only 12hrs. it's bright pink and makes the cakes stink like hell


this one was 48hrs after inoculation when the photos were taken.
i only sterilize by steaming in a large pot but did a control and steamed 4 jars then left them for 3 days and NO signs of contam.
if anyone could help me shed some light on this mongrel contam and how to try and avoid it. i flame sterilize after every inoculation site and dont know how to be any more sterile than i have been.
i'm at my wit's end!
-------------------- Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!
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MLBjammer
Invitro Freak


Registered: 02/24/06
Posts: 560
Loc: Southeastern USA
Last seen: 7 years, 14 days
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Re: repeated contamination [Re: soma_seeker]
#5759797 - 06/17/06 05:21 AM (17 years, 7 months ago) |
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Try a new source of spores and invest in a pressure cooker.
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: repeated contamination [Re: MLBjammer]
#5760242 - 06/17/06 10:21 AM (17 years, 7 months ago) |
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It's your LC. Try injecting the spores directly into your brf. If it contaminates then, you know your prints/syringe are contaminated. Ya gotta admit though, lipstick mold is kinda pretty. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Thai_connection
Siam


Registered: 10/01/05
Posts: 247
Loc: Thailand
Last seen: 13 years, 3 months
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Re: repeated contamination [Re: RogerRabbit]
#5760298 - 06/17/06 10:53 AM (17 years, 7 months ago) |
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yes pc does make a huge difference, also try not to sniff any contams,,,some are really bad/ check out the lc as rr mentioned too.
peace siam
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soma_seeker
Stranger in aStrange Land


Registered: 04/02/06
Posts: 367
Loc: Down under
Last seen: 3 months, 20 days
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yeh if i weren't so pissed off by the site of it i guess it would be pretty. 
i'm now out of spores but if i can get some more i'll try prep'ing a spore syring like u suggested RR. i've just read a couple of old posts were people left lipstick mold contam'd cakes and the myc took back over and killed it. my latest set of cakes have begun to colonise so i think i might let them go for a while and see how they end up. i have no uncontam'd cakes i risk infecting so i figure i have nothing to lose.
thanks guys
-------------------- Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!
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soma_seeker
Stranger in aStrange Land


Registered: 04/02/06
Posts: 367
Loc: Down under
Last seen: 3 months, 20 days
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Re: repeated contamination [Re: soma_seeker]
#5765894 - 06/18/06 10:56 PM (17 years, 7 months ago) |
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if you thought the last pic was pretty.....

its a shame lipstick mold isn't psychedelic cause i've got a shitload of it.. ;(
-------------------- Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!
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