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OfflineCepheus
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Green Mould
    #5685628 - 05/29/06 04:30 AM (17 years, 8 months ago)

I'm not sure wether this was my fault or the vendors ..

I inoculated 12 cakes about 3 - 4 days ago
4 with P. cubensis mexican
8 with P. cubensis burma
(both from mm.org)

I did them all at the same time and replaced the needle with a sterile medical needle (on both syringes) and flame sterilized after each inoculation... but the 4 with P. cubensis mexican are now infected with green mould...

any ideas what caused this?


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Invisiblemonstermitch
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Re: Green Mould [Re: Cepheus]
    #5686182 - 05/29/06 11:50 AM (17 years, 8 months ago)

If flaming is the only sterile procedure you used,
I would say your fault.

Need far more information on every detail of the inoculation.
Also, did any control jars contam?
Do you make control jars?

Where is the mold in the jar?
Glove box, flow hood, neither?
Alcohol pad wrapped around the needle at all times?
Still air or some movement in the air?

Lots of places you could have gone wrong.
More details please.


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OfflineRogerRabbitM
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Re: Green Mould [Re: monstermitch]
    #5686259 - 05/29/06 12:27 PM (17 years, 8 months ago)

If all four from one syringe contaminated and all eight from the other syringe are ok, it makes a strong case for a contaminated syringe. Just tell it to the vendor like you told it here, and I'm sure you'll get a replacement. Stuff happens.
RR


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OfflineYefet2000
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Re: Green Mould [Re: RogerRabbit]
    #5707001 - 06/03/06 10:25 AM (17 years, 7 months ago)

as RogerRabbit said. if the other 8 are ok, its the syringe.


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OfflineCepheus
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Re: Green Mould [Re: Yefet2000]
    #5711074 - 06/04/06 12:51 PM (17 years, 7 months ago)

Man I've been trying to get hold of the vendor for a while now to no avail.

dunno whats up with this... they are listed as a shroomery sponsor


--------------------
"I only ever hope to reach equilibrium, in Nature's matrix, in line with the meridian" ~ Jehst

:sun: "...and I know that I have to keep breathing, as tomorrow the sun will rise, who knows what the tide will bring?" :sun:

Free Spore Ring Europe
Send any spare spore prints you might have and help the distribution :grin:

Open Source. Freedom.  GNU/Linux

Addicting is not a word.


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