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Cepheus
Balance



Registered: 04/19/06
Posts: 8,266
Loc: the space between reality...
Last seen: 1 year, 1 month
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Green Mould
#5685628 - 05/29/06 04:30 AM (17 years, 8 months ago) |
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I'm not sure wether this was my fault or the vendors ..
I inoculated 12 cakes about 3 - 4 days ago 4 with P. cubensis mexican 8 with P. cubensis burma (both from mm.org)
I did them all at the same time and replaced the needle with a sterile medical needle (on both syringes) and flame sterilized after each inoculation... but the 4 with P. cubensis mexican are now infected with green mould...
any ideas what caused this?
-------------------- "I only ever hope to reach equilibrium, in Nature's matrix, in line with the meridian" ~ Jehst
"...and I know that I have to keep breathing, as tomorrow the sun will rise, who knows what the tide will bring?" Free Spore Ring Europe Send any spare spore prints you might have and help the distribution
Open Source. Freedom. GNU/Linux Addicting is not a word.
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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Re: Green Mould [Re: Cepheus]
#5686182 - 05/29/06 11:50 AM (17 years, 8 months ago) |
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If flaming is the only sterile procedure you used, I would say your fault.
Need far more information on every detail of the inoculation. Also, did any control jars contam? Do you make control jars?
Where is the mold in the jar? Glove box, flow hood, neither? Alcohol pad wrapped around the needle at all times? Still air or some movement in the air?
Lots of places you could have gone wrong. More details please.
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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If all four from one syringe contaminated and all eight from the other syringe are ok, it makes a strong case for a contaminated syringe. Just tell it to the vendor like you told it here, and I'm sure you'll get a replacement. Stuff happens. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Yefet2000
HolyLandShroom


Registered: 08/24/05
Posts: 172
Loc: IsraHell
Last seen: 1 month, 26 days
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as RogerRabbit said. if the other 8 are ok, its the syringe.
-------------------- Me fail english ? Thats unpossible...
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Cepheus
Balance



Registered: 04/19/06
Posts: 8,266
Loc: the space between reality...
Last seen: 1 year, 1 month
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Re: Green Mould [Re: Yefet2000]
#5711074 - 06/04/06 12:51 PM (17 years, 7 months ago) |
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Man I've been trying to get hold of the vendor for a while now to no avail.
dunno whats up with this... they are listed as a shroomery sponsor
-------------------- "I only ever hope to reach equilibrium, in Nature's matrix, in line with the meridian" ~ Jehst
"...and I know that I have to keep breathing, as tomorrow the sun will rise, who knows what the tide will bring?" Free Spore Ring Europe Send any spare spore prints you might have and help the distribution
Open Source. Freedom. GNU/Linux Addicting is not a word.
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