Home | Community | Message Board


Phytoextractum
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: Agar

Jump to first unread post. Pages: 1
InvisibleJoshua
Holoman
Male

Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Make your own innoculation loop.
    #532563 - 01/27/02 02:20 AM (15 years, 7 months ago)

1) Start with a twisty tie, the kind you close bread up with, the longer the better.

2) Strip the plastic covering off.

3) Bend gently in half.

4) Place bend over round style pencil/pen so that you can balance the wire on the pencil. The pencil/pen will form the loop.

5) Twist the two ends together starting closest to the pencil/pen.

6) Continue to twist tightly until two tails are left. Approximately 1 inch long.

7) Remove wire from pencil/pen. This should require slipping the wire loop down the length of the pencil/pen.

8) Wrap wire tails around tip of pencil. To tighten, twist wires together.

9) To make sure that wire is fixed to pencil a peice of tape may help.



I hope this helps you all to manufacture your own innoculation loop. Hopefully eventually you will be able to afford this $3-4 item.



Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineCabee
newbie
Registered: 09/24/01
Posts: 49
Last seen: 15 years, 7 months
Re: Make your own innoculation loop. [Re: Joshua]
    #532726 - 01/27/02 05:39 AM (15 years, 7 months ago)

Stupid question here.What's it for?


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleJoshua
Holoman
Male

Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: Make your own innoculation loop. [Re: Cabee]
    #532728 - 01/27/02 05:42 AM (15 years, 7 months ago)

It is used in agar cultivation to transfer spores to the agar medium.

Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJammer
Computers areMORE Addictive!

Registered: 11/05/00
Posts: 3,998
Loc: (God's Country) - USA
Last seen: 12 years, 1 day
Re: Make your own innoculation loop. [Re: Joshua]
    #532729 - 01/27/02 05:43 AM (15 years, 7 months ago)

Now thats the kind of stuff that we need more of here. (seriously)

I have mega respect for most Vendors on this site, however I also like information that can save money. (personaly, my friends tell me that I'm a great hand at makeing home-made bud pipes from almost anything non toxic)


--------------------
>>Jammer>>


Edited by Jammer (01/27/02 05:45 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineCabee
newbie
Registered: 09/24/01
Posts: 49
Last seen: 15 years, 7 months
Re: Make your own innoculation loop. [Re: Joshua]
    #532735 - 01/27/02 05:59 AM (15 years, 7 months ago)

Can you explain the reason why someone would use spores for agar cultivation over cakes or caseings?


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinefresh357
addict

Registered: 09/02/01
Posts: 493
Last seen: 15 years, 5 months
Re: Make your own innoculation loop. [Re: Cabee]
    #532737 - 01/27/02 06:02 AM (15 years, 7 months ago)

Agar is an advanced technique used for strain isolation. Please do some reading.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJammer
Computers areMORE Addictive!

Registered: 11/05/00
Posts: 3,998
Loc: (God's Country) - USA
Last seen: 12 years, 1 day
Re: Make your own innoculation loop. [Re: fresh357]
    #532740 - 01/27/02 06:06 AM (15 years, 7 months ago)

AH...

W-O-R-D-S


haha


--------------------
>>Jammer>>


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleJoshua
Holoman
Male

Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: Make your own innoculation loop. [Re: Cabee]
    #532743 - 01/27/02 06:10 AM (15 years, 7 months ago)

Agar has a few specialised applications.

1) It allows one to isolate strains. By letting the mycelium grow out you can visually see which areas are growing rhizomorphically. Generally these areas are signs of aggresive strains.

2) It allows you to work with contaminated prints. By growing the prints out on agar you will be able to isolate healthy non-cantaminated areas of mycelium. You would then remove these areas w/ a scalpel and transfer to a fresh plate.

3) Cloning. You can take flesh or mycelium from a desired fruit or strain and grow it out. All of the mycelium from this original is geneticaly identical to the original.

4) etc. These are just a few advantages to agar culturing.

Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleJoshua
Holoman
Male

Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: Make your own innoculation loop. [Re: fresh357]
    #532752 - 01/27/02 06:36 AM (15 years, 7 months ago)

Take it easy there thunder. I posted in general cultivation. This guy stuck to his area, it's probably my fault for posting in this forum. As you said agar is an "advanced technique".

Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,663
Loc: Montana
Trusted Cultivator
Re: Make your own innoculation loop. [Re: Joshua]
    #532753 - 01/27/02 06:38 AM (15 years, 7 months ago)

Great idea Josh !
Another toy for my lab....lolz
I love the home made chit too.
Maybe i will use some thin Stainless wire that i have laying around.
The stainless won't rust like the mild steel.
Thanks for the great idea....buddy.
I'll make you one Josh.


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



Post Extras: Print Post  Remind Me! Notify Moderator
Offlinefresh357
addict

Registered: 09/02/01
Posts: 493
Last seen: 15 years, 5 months
Re: Make your own innoculation loop. [Re: Joshua]
    #532754 - 01/27/02 06:41 AM (15 years, 7 months ago)

(shrugs) well since you dont mind explaining the basic concepts of agar everytime you post i guess theres no problem.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJammer
Computers areMORE Addictive!

Registered: 11/05/00
Posts: 3,998
Loc: (God's Country) - USA
Last seen: 12 years, 1 day
Re: Make your own innoculation loop. [Re: fresh357]
    #532762 - 01/27/02 06:59 AM (15 years, 7 months ago)

With all due respect...

This thread seems to have encountered some sort of "bug" or "glitch".....

There are a few replies that have disapered from a couple of hours ago from within this thread.

The reference to "basic concepts of agar" was perfectly in context to what the thread was before. (it dosent "read" the same way now)


--------------------
>>Jammer>>


Edited by Jammer (01/27/02 06:59 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleZen Peddler
Male User Gallery

Registered: 06/18/01
Posts: 6,379
Loc: orbit
Re: Make your own innoculation loop. [Re: Joshua]
    #532783 - 01/27/02 07:52 AM (15 years, 7 months ago)

great idea Joshua - money saving is always nice when you just quit your job :-)


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblear393
old timer
Male
Registered: 01/27/00
Posts: 702
Loc: VT
Re: Make your own innoculation loop. [Re: Zen Peddler]
    #534108 - 01/28/02 05:06 PM (15 years, 7 months ago)

or just got canned....my case...
peace, ar393
any one hiring IT people? i just made a funny....


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* DIY innoculation loop instructions? oscill8 2,532 2 09/08/01 09:51 AM
by Papa_Bear
* What can I use instead of an innoculating loop? creekfreek 2,181 19 12/24/08 10:45 AM
by BenDover
* Streaking technique and Plastic inoculation loops mesq 2,030 7 04/12/03 12:27 PM
by no-tone
* Homemade innoc. loop Dragonaut 654 1 01/09/06 02:22 AM
by BataviaVakereli
* Problems with Agar Innoculation? sparafucile 813 3 02/17/02 11:48 AM
by kayaman9
* A quick homeade lnoculation loop for streaking arp180 265 1 12/29/08 06:03 PM
by Damion5050
* Inoculating Loop substitute. trich_cultivator 1,623 5 11/24/08 07:43 PM
by trich_cultivator
* innoculating with prints tryptonite 561 2 04/21/04 12:05 PM
by ThatDaltonGuy

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
1,700 topic views. 12 members, 50 guests and 30 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
FreeSpores.com
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.005 seconds on 16 queries.