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 Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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 Make your own innoculation loop.   1
#532563 - 01/27/02 12:20 AM (21 years, 10 months ago) |
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1) Start with a twisty tie, the kind you close bread up with, the longer the better.
2) Strip the plastic covering off.
3) Bend gently in half.
4) Place bend over round style pencil/pen so that you can balance the wire on the pencil. The pencil/pen will form the loop.
5) Twist the two ends together starting closest to the pencil/pen.
6) Continue to twist tightly until two tails are left. Approximately 1 inch long.
7) Remove wire from pencil/pen. This should require slipping the wire loop down the length of the pencil/pen.
8) Wrap wire tails around tip of pencil. To tighten, twist wires together.
9) To make sure that wire is fixed to pencil a peice of tape may help.
I hope this helps you all to manufacture your own innoculation loop. Hopefully eventually you will be able to afford this $3-4 item.
Joshua
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The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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 Cabee
newbie
Registered: 09/24/01
Posts: 49
Last seen: 21 years, 10 months
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 Re: Make your own innoculation loop. [Re: Joshua]
#532726 - 01/27/02 03:39 AM (21 years, 10 months ago) |
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Stupid question here.What's it for?
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Re: Make your own innoculation loop. [Re: Cabee]
#532728 - 01/27/02 03:42 AM (21 years, 10 months ago) |
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It is used in agar cultivation to transfer spores to the agar medium.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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Jammer
Computers areMORE Addictive!
Registered: 11/04/00
Posts: 3,998
Loc: (God's Country) - USA
Last seen: 18 years, 2 months
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 Re: Make your own innoculation loop. [Re: Joshua]
#532729 - 01/27/02 03:43 AM (21 years, 10 months ago) |
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Now thats the kind of stuff that we need more of here. (seriously)
I have mega respect for most Vendors on this site, however I also like information that can save money. (personaly, my friends tell me that I'm a great hand at makeing home-made bud pipes from almost anything non toxic)
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>>Jammer>>
Edited by Jammer (01/27/02 03:45 AM)
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Cabee
newbie
Registered: 09/24/01
Posts: 49
Last seen: 21 years, 10 months
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 Re: Make your own innoculation loop. [Re: Joshua]
#532735 - 01/27/02 03:59 AM (21 years, 10 months ago) |
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Can you explain the reason why someone would use spores for agar cultivation over cakes or caseings?
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fresh357
addict
Registered: 09/02/01
Posts: 493
Last seen: 21 years, 7 months
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Re: Make your own innoculation loop. [Re: Cabee]
#532737 - 01/27/02 04:02 AM (21 years, 10 months ago) |
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Agar is an advanced technique used for strain isolation. Please do some reading.
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Jammer
Computers areMORE Addictive!
Registered: 11/04/00
Posts: 3,998
Loc: (God's Country) - USA
Last seen: 18 years, 2 months
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Re: Make your own innoculation loop. [Re: fresh357]
#532740 - 01/27/02 04:06 AM (21 years, 10 months ago) |
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AH...
W-O-R-D-S
haha
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>>Jammer>>
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Re: Make your own innoculation loop. [Re: Cabee]
#532743 - 01/27/02 04:10 AM (21 years, 10 months ago) |
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Agar has a few specialised applications.
1) It allows one to isolate strains. By letting the mycelium grow out you can visually see which areas are growing rhizomorphically. Generally these areas are signs of aggresive strains.
2) It allows you to work with contaminated prints. By growing the prints out on agar you will be able to isolate healthy non-cantaminated areas of mycelium. You would then remove these areas w/ a scalpel and transfer to a fresh plate.
3) Cloning. You can take flesh or mycelium from a desired fruit or strain and grow it out. All of the mycelium from this original is geneticaly identical to the original.
4) etc. These are just a few advantages to agar culturing.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Re: Make your own innoculation loop. [Re: fresh357]
#532752 - 01/27/02 04:36 AM (21 years, 10 months ago) |
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Take it easy there thunder. I posted in general cultivation. This guy stuck to his area, it's probably my fault for posting in this forum. As you said agar is an "advanced technique".
Joshua
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The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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 Re: Make your own innoculation loop. [Re: Joshua]
#532753 - 01/27/02 04:38 AM (21 years, 10 months ago) |
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Great idea Josh !
Another toy for my lab....lolz
I love the home made chit too.
Maybe i will use some thin Stainless wire that i have laying around.
The stainless won't rust like the mild steel.
Thanks for the great idea....buddy.
I'll make you one Josh.
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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fresh357
addict
Registered: 09/02/01
Posts: 493
Last seen: 21 years, 7 months
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Re: Make your own innoculation loop. [Re: Joshua]
#532754 - 01/27/02 04:41 AM (21 years, 10 months ago) |
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(shrugs) well since you dont mind explaining the basic concepts of agar everytime you post i guess theres no problem.
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Jammer
Computers areMORE Addictive!
Registered: 11/04/00
Posts: 3,998
Loc: (God's Country) - USA
Last seen: 18 years, 2 months
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Re: Make your own innoculation loop. [Re: fresh357]
#532762 - 01/27/02 04:59 AM (21 years, 10 months ago) |
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With all due respect...
This thread seems to have encountered some sort of "bug" or "glitch".....
There are a few replies that have disapered from a couple of hours ago from within this thread.
The reference to "basic concepts of agar" was perfectly in context to what the thread was before. (it dosent "read" the same way now)
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>>Jammer>>
Edited by Jammer (01/27/02 04:59 AM)
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Zen Peddler
Registered: 06/18/01
Posts: 6,379
Loc: orbit
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 Re: Make your own innoculation loop. [Re: Joshua]
#532783 - 01/27/02 05:52 AM (21 years, 10 months ago) |
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great idea Joshua - money saving is always nice when you just quit your job :-)
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ar393
old timer
Registered: 01/26/00
Posts: 702
Loc: VT
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Re: Make your own innoculation loop. [Re: Zen Peddler]
#534108 - 01/28/02 03:06 PM (21 years, 10 months ago) |
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or just got canned....my case...
peace, ar393
any one hiring IT people? i just made a funny....
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