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OfflineNiGGy
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Registered: 02/10/02
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Loc: Nova Scotia, Canada
Last seen: 18 years, 3 months
Would this work to innoculate grain?
    #1189639 - 01/04/03 07:24 AM (21 years, 5 months ago)

Taking A BRF cake and grating some of it and putting it in the jar with grain. Give it a shake and wait for it colonize?

I want a way to innoculate grain without using agar and all that. I don't have the money.

Or would innoculating honey water with a syringe do the job. Ones there is mycelium in the jar innoculate the grain with it.

Edited by NiGGy (01/04/03 07:36 AM)

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OfflineLoop_Theorist
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Registered: 04/22/02
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Re: Would this work to innoculate grain? [Re: NiGGy]
    #1189727 - 01/04/03 07:56 AM (21 years, 5 months ago)

Just Innoculate the grain with a spore syringe after you P.C your grain jars...


-Loop


--------------------
Pack-tight, midnight, ship it to tha coast.
Some take tha plane, you, you on tha boat,
right beside tha money which right beside tha coke
Passin by tha coastgaurd, right under their nose.

To the US-HEY where anyone can getny, MIA all the way to New Your city, you a twenty now....

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Anonymous

Re: Would this work to innoculate grain? [Re: NiGGy]
    #1189746 - 01/04/03 08:04 AM (21 years, 5 months ago)

Yes you can innoculate grain with a BRF cake. The mycelium water works well too. Innoculating with a cake should work faster as there is more mycelium. With one 1/2 pint BRF cake you could easily start 5 pints of grain.

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OfflineNiGGy
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Re: Would this work to innoculate grain? [Re: Loop_Theorist]
    #1189774 - 01/04/03 08:21 AM (21 years, 5 months ago)

Quote:

Just Innoculate the grain with a spore syringe after you P.C your grain jars...


-Loop




The spores won't germinate.


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OfflineNiGGy
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Re: Would this work to innoculate grain? [Re: ]
    #1189781 - 01/04/03 08:24 AM (21 years, 5 months ago)

Quote:

Yes you can innoculate grain with a BRF cake. The mycelium water works well too. Innoculating with a cake should work faster as there is more mycelium. With one 1/2 pint BRF cake you could easily start 5 pints of grain. 




I think I will try mycelium water to innoculate the grain...

And then with one jar of grain you can innoculate other jars..With those jars you can innoculate more jars. Kinda like mitosis  :smirk: 

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Invisibledog
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Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: Would this work to innoculate grain? [Re: NiGGy]
    #1189807 - 01/04/03 08:39 AM (21 years, 5 months ago)

Quote:

The spores won't germinate.



Nonsense.


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.

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Offlinedebianlinux
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Re: Would this work to innoculate grain? [Re: dog]
    #1189880 - 01/04/03 09:06 AM (21 years, 5 months ago)

not entirely nonsense. mostly nonsense tho. the spores DO have to be "next" to each other to mate/germinate and shooting a weak syringe into a quart of jar of grrain raises the possibility of the spores being too far from each other to mate/germinate. that being said, that is not a very large possibility.

also BRF is grain. what with all the talk of grain to grain transfer, you'd think that transferring BRF (grain) to rye (grain) would work, right? especially since any amount of searching will show that many people, including myself, have posted great results with this method.

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Invisiblezeta
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Registered: 05/24/02
Posts: 3,972
Re: Would this work to innoculate grain? [Re: debianlinux]
    #1190033 - 01/04/03 10:20 AM (21 years, 5 months ago)

I have had nothing but success with injecting spores into grain jars.

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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: Would this work to innoculate grain? [Re: zeta]
    #1190036 - 01/04/03 10:22 AM (21 years, 5 months ago)

What are the odds that two spores won't be right next to each other. In other words, injecting spore solution will defenitely work.


--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.

Edited by SixCee (01/04/03 10:23 AM)

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OfflineNiGGy
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Re: Would this work to innoculate grain? [Re: zeta]
    #1190057 - 01/04/03 10:30 AM (21 years, 5 months ago)

Really?

So I don't need to work with agar or anything??

So I can just PC some grain, innoculate it with a normal spore syringe. Wait utill it colonizes. And then spawn pasteurized straw with it?

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InvisibleSixCee
keep rolling
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Registered: 06/12/02
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Loc: US, Chicago
Re: Would this work to innoculate grain? [Re: NiGGy]
    #1190072 - 01/04/03 10:37 AM (21 years, 5 months ago)

No. Unless you want to make an isolate.


--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.

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OfflineNiGGy
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Re: Would this work to innoculate grain? [Re: SixCee]
    #1190116 - 01/04/03 10:53 AM (21 years, 5 months ago)

An isolate?


Please tell me the best way of going about what I want to do. Thanks.  :smile: 

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OfflineSkikid16
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Re: Would this work to innoculate grain? [Re: NiGGy]
    #1190136 - 01/04/03 11:00 AM (21 years, 5 months ago)

Don't worry about an isolate. Just do a multispore inoculation with a spore syringe, you will be fine, there is little to no chance that the spores will not germinate.


--------------------
Re-Defeat Bush in '04

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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: Would this work to innoculate grain? [Re: NiGGy]
    #1190137 - 01/04/03 11:01 AM (21 years, 5 months ago)

Put some mycelium in sterilized agar in a sterile environment and watch the mycelim grow.

Thats an isolate. You isolated the strain.


--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.

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OfflineNiGGy
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Re: Would this work to innoculate grain? [Re: SixCee]
    #1190176 - 01/04/03 11:20 AM (21 years, 5 months ago)

Alright! Thanks!

So mycelium water, transfer, spore syringe, will all innoculate grain ( birdseed , rye , etc )


Edited by NiGGy (01/04/03 11:22 AM)

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OfflineSkikid16
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Re: Would this work to innoculate grain? [Re: NiGGy]
    #1190183 - 01/04/03 11:23 AM (21 years, 5 months ago)

Quote:

So mycelium water, transfer, spore syringe, will all innoculate grain ( birdseed , rye , etc )


Yup


--------------------
Re-Defeat Bush in '04

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OfflineSkryllz
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Registered: 03/25/02
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Re: Would this work to innoculate grain? [Re: Skikid16]
    #1190291 - 01/04/03 12:28 PM (21 years, 5 months ago)

Not only will multispore innoculation work on birseed, it should work fast. In the past week I've done 20+ pints of birdseed with mutispore innoculation(4 different strains) and have seen growth on all of them within 48 hours.

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OfflineNiGGy
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Re: Would this work to innoculate grain? [Re: Skryllz]
    #1190317 - 01/04/03 12:40 PM (21 years, 5 months ago)

Quote:

Not only will multispore innoculation work on birseed, it should work fast. In the past week I've done 20+ pints of birdseed with mutispore innoculation(4 different strains) and have seen growth on all of them within 48 hours.




SWEET!!

Is birdseed big enough though? The seeds are so small in it. Would it colonize slowly since they are so compact and small? And I can't see it shaking too well either. I picture it in clumps.

Edited by NiGGy (01/04/03 01:00 PM)

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OfflineSkikid16
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Re: Would this work to innoculate grain? [Re: NiGGy]
    #1190326 - 01/04/03 12:45 PM (21 years, 5 months ago)

Quote:

Is birdseed big enough though?


I use pennington's wild birdseed. works good for me. And what do you mean by big enough? If size of substrate was important, how could you use brown rice flour?


--------------------
Re-Defeat Bush in '04

Edited by Skikid16 (01/04/03 12:46 PM)

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OfflineSkryllz
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Registered: 03/25/02
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Re: Would this work to innoculate grain? [Re: NiGGy]
    #1190330 - 01/04/03 12:48 PM (21 years, 5 months ago)

Yes it's big enough. It is also usually the cheapest of all grains usually costing under $.50/lb. Soak it overnight, load into jars until they are about 3/4 full, pressure cook, let cool and innoculate. I read in another post that you don't know what psi your pc cooks at so I would cook the jars for 80-90 minutes, atleast thats what I do with my 10psi pc.

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