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Offlinechillin48
newbie
Registered: 12/11/01
Posts: 30
Last seen: 14 years, 7 months
no growth from clear syringes
    #523091 - 01/17/02 03:06 AM (14 years, 10 months ago)

My buddy made his own syringes that looked clear. I read in other posts that
even with clear syringes, everything goes well. But only four of his jars (out of 12)
are showing growth after a week. Has anyone ever had this happen. Will the rest of them eventually do well. Thanks


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InvisibleRoadkill
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Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
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Re: no growth from clear syringes [Re: chillin48]
    #523119 - 01/17/02 04:05 AM (14 years, 10 months ago)

What are his temps in his incubator?
Might be that.....might have stalled?
Might have too dry of substrate.
Do you see any condensation in the jars?
Temps should be 84 to 86 degrees.


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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InvisibleWakingUpLate
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Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
Re: no growth from clear syringes [Re: chillin48]
    #523156 - 01/17/02 05:10 AM (14 years, 10 months ago)

Did he continue to shake his syringe so that
they all didn't settle to the tip of the syringe?


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)


Edited by WakingUpLate (01/17/02 05:12 AM)


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Anonymous

Re: no growth from clear syringes [Re: chillin48]
    #523224 - 01/17/02 08:21 AM (14 years, 10 months ago)

Certain strains produce lighter or darker syringes. For instance, if I make a syringe of Ecuador and a syringe of GT and I use the exact same amount of spores, you would look at the Ecuador and comment how "dark and sporulated" it is. When you look at the GT syringe, you would say it looked like water, when in reality they both contain the same amount of spores. If some of your jars colonized, then your syringe was probably fine. I would look to your substrate as the cause of your problem. IMO


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Anonymous

Re: no growth from clear syringes [Re: ]
    #523391 - 01/17/02 11:39 AM (14 years, 10 months ago)

Substrate sound fine considering some are colonizing. I would say the syringes were either 'weak' or didn't get shaken up and all the spores were injected into the first 4 jars.

Some of those other jars may come through yet, be patient!


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Anonymous

Re: no growth from clear syringes [Re: ]
    #523397 - 01/17/02 11:54 AM (14 years, 10 months ago)

Yeah, your probably right Max. It most likely is the lack of shaking the syringe. I have seen wide variations in water content of jars though.


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Offlinechillin48
newbie
Registered: 12/11/01
Posts: 30
Last seen: 14 years, 7 months
Re: no growth from clear syringes [Re: ]
    #523631 - 01/17/02 05:56 PM (14 years, 10 months ago)

He made sure to shake the syringes after every injection. The temps wouldnt really be the problem since they are all together, and 4 are doing well.


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OfflineJammer
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Re: no growth from clear syringes [Re: chillin48]
    #524967 - 01/18/02 10:10 PM (14 years, 10 months ago)

I understand that even the best syringies can sometimes (for various reasons) MIGHT take weeks to kick in (these are the exceptions) before any growth shows. Often old sryingies can take longer (like I heard of one inoculation with a vendor's sryinge taking over a month to show any sign of mycelium growth on PF cakes).


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>>Jammer>>


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InvisibleAzulAgave
member
Registered: 10/18/01
Posts: 170
Re: no growth from clear syringes [Re: chillin48]
    #525029 - 01/18/02 11:17 PM (14 years, 10 months ago)

I just had the same problem. The only thing is when I made my jars I lightly packed the substrate. Packing the substrate cuts the O2 off to the cake and stops mycelium. That the was the firstand last time I'll lightly pack the substrate into the jar.


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