Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

PlanetSpores.ca
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Advanced Mycology
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Sporeworks.EU Spores for European Microscopy   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Substrate Bags

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
agar contams
    #519562 -

made 10 dishes with malt yeast agar mix, used tissue samples. all started but i lost 8 of the 10 to a mold that ruined my last attemp also. its a light foamy like mold(almost like hair) with black speks in it(spores?) anyone else gotn this? what is it? noone in contam fourm could help any


--------------------
pleezr

"Tell me about the fucking golf shoes!!"

Extras: Filter Print Post Top
Re: agar contams [Re: pleezr] * 1
    #519568 -

are you using a glove box or the oven tek??

Do you already have cultures on dishes isolated and such if so give some h2o2 MEA a try


--------------------
Dr.Bluethumbs
RAFFLE to help out GS and his 7 month old daughter

Extras: Filter Print Post Top
Re: agar contams [Re: pleezr]
    #519611 -

>what is it?
It sounds like aspergilus mold, can be dangerous if you inhale the spores.


--------------------
Grow Mushrooms from Spores - PF-Tek for Simple Minds

Extras: Filter Print Post Top
Re: agar contams [Re: dimitri211]
    #519650 -

I didnt use a glovebox, and thatts prob why my contam rate was so high. I accually just did the tissue sample right on my kitchen counter(yeah I know)But thats why we make so many dishes right? this contam is the #1 mold i seem to get the most frequently on agar, it is elevated of the agar kinda high, sorta like soap suds.

and no its not a isolated yet, its on clean agar(first agar inoc)


--------------------
pleezr

"Tell me about the fucking golf shoes!!"

Edited by pleezr (01/13/02 05:00 PM)

Extras: Filter Print Post Top
Re: agar contams [Re: pleezr]
    #519670 -

Do all your agar work w/ the oven tek,clean hands and a face mask (or do not breathe directly on it)
This should help.


--------------------
Dr.Bluethumbs
RAFFLE to help out GS and his 7 month old daughter

Extras: Filter Print Post Top
Re: agar contams [Re: dimitri211]
    #520205 -

100 latex gloves $ 6.95 at wall mart. You can build a glovebox for under $30, If you have a little extra cash it is well worth your time.

Extras: Filter Print Post Top
Re: agar contams [Re: Chemical_Smile]
    #520221 -

But if you live in a 25m^2 appartement you have no place to put that thing.

zilch

Extras: Filter Print Post Top
Re: agar contams [Re: zilch]
    #520507 -

clena your ktichen better, my cat has never had major contam problems (due to environment anyways) and has always worked on her kitchen counter.
peace, ar393

Extras: Filter Print Post Top
Re: agar contams [Re: pleezr]
    #520524 -

Actually, if it's really hairy as you describe--possibly even pushing up against the top of the dish, you likely have one of two things;

Mucor sp. or Rhizopus sp.

Not that it makes much difference, pitch ASAP;

Extras: Filter Print Post Top
Re: agar contams [Re: Suntzu]
    #520529 -

Also, because these guys get so extremely aerial so fast, it's important to tape the plates shut if you have any question about it [masking tape works well, fold an end so you can remove easily]. Storing a Mucor plate near clean plates can still cross-contaminate if there is ANY lifting of the lid due to the growth of the mold. This can even occur overnight, it grows so fast!
May not be necessary if you use heavy glass/pyrex petris.

Edited by Suntzu (01/14/02 02:08 PM)

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Sporeworks.EU Spores for European Microscopy   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Substrate Bags

Mushrooms, Mycology and Psychedelics > Advanced Mycology
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* my insaine agar contam blaster idea. Shaw 613 2 07/28/02 11:21 AM
by Anonymous
* Agar Strain Isolation - Is it worth it?
( 1 2 all )		 Blue Helix 13,949 20 02/23/05 01:07 AM
by Blue Helix
* Re: I found out I'm getting an agar set for xmas! Anonymous 1,976 10 12/22/99 05:22 PM
by AnubisRonin
* Agar Medium EvilGir 1,475 4 01/31/02 08:21 PM
by JuSMe13
* Questions On Agar ParticleMan 3,558 13 03/27/02 10:11 PM
by Shaw
* Glovebox critiscism/questions? socratesmind 1,127 2 10/07/02 09:23 PM
by Green_Velvet
* Agar n Open air? socratesmind 2,637 4 01/03/03 08:57 AM
by mycofile
* green agar pj541 1,393 6 09/30/01 01:30 PM
by pj541

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta
1,490 topic views. 0 members, 4 guests and 7 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.006 seconds on 16 queries.