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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Offlinepleezr
fuct

Registered: 12/22/01
Posts: 1,708
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Last seen: 20 years, 1 month
agar contams
    #519562 - 01/13/02 03:12 PM (23 years, 2 months ago)

made 10 dishes with malt yeast agar mix, used tissue samples. all started but i lost 8 of the 10 to a mold that ruined my last attemp also. its a light foamy like mold(almost like hair) with black speks in it(spores?) anyone else gotn this? what is it? noone in contam fourm could help any


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pleezr

"Tell me about the fucking golf shoes!!"

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Invisibledimitri211
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Registered: 04/27/01
Posts: 2,248
Re: agar contams [Re: pleezr] * 1
    #519568 - 01/13/02 03:18 PM (23 years, 2 months ago)

are you using a glove box or the oven tek??

Do you already have cultures on dishes isolated and such if so give some h2o2 MEA a try


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Dr.Bluethumbs
RAFFLE to help out GS and his 7 month old daughter

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OfflineAnnoA
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Re: agar contams [Re: pleezr]
    #519611 - 01/13/02 04:00 PM (23 years, 2 months ago)

>what is it?
It sounds like aspergilus mold, can be dangerous if you inhale the spores.


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Grow Mushrooms from Spores - PF-Tek for Simple Minds

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Offlinepleezr
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Registered: 12/22/01
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Last seen: 20 years, 1 month
Re: agar contams [Re: dimitri211]
    #519650 - 01/13/02 04:57 PM (23 years, 2 months ago)

I didnt use a glovebox, and thatts prob why my contam rate was so high. I accually just did the tissue sample right on my kitchen counter(yeah I know)But thats why we make so many dishes right? this contam is the #1 mold i seem to get the most frequently on agar, it is elevated of the agar kinda high, sorta like soap suds.

and no its not a isolated yet, its on clean agar(first agar inoc)


--------------------
pleezr

"Tell me about the fucking golf shoes!!"

Edited by pleezr (01/13/02 05:00 PM)

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Invisibledimitri211
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Registered: 04/27/01
Posts: 2,248
Re: agar contams [Re: pleezr]
    #519670 - 01/13/02 05:21 PM (23 years, 2 months ago)

Do all your agar work w/ the oven tek,clean hands and a face mask (or do not breathe directly on it)
This should help.


--------------------
Dr.Bluethumbs
RAFFLE to help out GS and his 7 month old daughter

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OfflineChemical_Smile
Making Love WithMy Ego
Registered: 09/20/01
Posts: 2,217
Loc: coming down fast, miles a...
Last seen: 21 years, 4 months
Re: agar contams [Re: dimitri211]
    #520205 - 01/14/02 08:08 AM (23 years, 2 months ago)

100 latex gloves $ 6.95 at wall mart. You can build a glovebox for under $30, If you have a little extra cash it is well worth your time.

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Invisiblezilch
enthusiast
Registered: 11/01/00
Posts: 58
Re: agar contams [Re: Chemical_Smile]
    #520221 - 01/14/02 08:34 AM (23 years, 2 months ago)

But if you live in a 25m^2 appartement you have no place to put that thing.

zilch

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Invisiblear393
old timer
Male
Registered: 01/26/00
Posts: 702
Loc: VT
Re: agar contams [Re: zilch]
    #520507 - 01/14/02 01:48 PM (23 years, 2 months ago)

clena your ktichen better, my cat has never had major contam problems (due to environment anyways) and has always worked on her kitchen counter.
peace, ar393

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OfflineSuntzu
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Registered: 10/14/99
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Re: agar contams [Re: pleezr]
    #520524 - 01/14/02 02:02 PM (23 years, 2 months ago)

Actually, if it's really hairy as you describe--possibly even pushing up against the top of the dish, you likely have one of two things;

Mucor sp. or Rhizopus sp.

Not that it makes much difference, pitch ASAP;

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OfflineSuntzu
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Registered: 10/14/99
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Re: agar contams [Re: Suntzu]
    #520529 - 01/14/02 02:06 PM (23 years, 2 months ago)

Also, because these guys get so extremely aerial so fast, it's important to tape the plates shut if you have any question about it [masking tape works well, fold an end so you can remove easily]. Storing a Mucor plate near clean plates can still cross-contaminate if there is ANY lifting of the lid due to the growth of the mold. This can even occur overnight, it grows so fast!
May not be necessary if you use heavy glass/pyrex petris.

Edited by Suntzu (01/14/02 02:08 PM)

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