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Offlinepj541
member
Registered: 02/04/01
Posts: 128
Loc: fuck off
Last seen: 14 years, 7 months
green agar
    #405323 - 09/26/01 09:34 PM (15 years, 2 months ago)

Hello, I was hoping that someone with experiance with agar could give me their oppinion.
My friend has read the mushroom cultivator along with hundreds of other documents and decided to try agar.
He poored 17 dishes in a glove box (the box doesen't have gloves, just holes). Once cool he innoculated 5 dishes with a eq print from ralph. he used disposable inocc loops (package sterile untell opened).
When he was done with that he wraped all 17 dishes with strips of 2" siran.

all was done in a simi sterile enviroment ie clorox disinfectant every where including in glovebox.

any way 3 of 5 grew large amounts of green mold in the center of each dish.

Question.
Were the contams from the sporeprint or from the air.

i know you can only guess
but pease do
thanks pj


"If you are frightened of the end and your holding on you see devils tearing your life away but if you've made your peace then the devils are really angels freeing you from the earth.."

DON'T ORDER FROM STP!!!!


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?Well, this is how the world works. All energies flow according to the whims of the great magnet. What a fool I was to defy him.?



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OfflineJahson
amigo de hongos
Registered: 10/10/00
Posts: 84
Last seen: 13 years, 1 month
Re: green agar [Re: pj541]
    #406030 - 09/27/01 12:09 PM (15 years, 2 months ago)

I guess you sterilized the agar for 30 min at 15 psi? If so and you used good sterile technique then it may have been the spores. You never know with aspirgillus. I got a contaminated pes amazonian from the fsr. Oh well...It was from 5 years back. I have only done about 5 plates of it and not one spore germinated on MYA. It happens. Any one have luck with his Wild cyans yet?

Enjoy FSR


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Enjoy FSR


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InvisibleDidjeridoo
Stranger

Registered: 08/03/01
Posts: 69
Re: green agar [Re: Jahson]
    #406248 - 09/27/01 03:28 PM (15 years, 2 months ago)

Jahson, with older spore prints, I usually have much better luck if they are allowed to rehydrate in a small amount of sterile water for 24 hrs. before inoculating. Since your working with agar, a pipette can be used to transfer a couple drops of spore water onto the agar.

Support the FSR:smile:


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Offlineduncan8r
Scum

Registered: 08/07/01
Posts: 91
Loc: Syd, Australia
Last seen: 1 year, 1 month
Re: green agar [Re: Didjeridoo]
    #407340 - 09/28/01 10:33 AM (15 years, 2 months ago)

If possible, repeat the agar tek u used but dont innoc the plates. Let them sit for a few days and any contams will show up fairly quick - this will give you an indication of how sterile you are being. Get some gloves if possible stops skin flakes falling onto the agar.

Best of luck

Duncan8r and his wet monkey

Not all chemicals are bad. Without chemicals such as hydrogen and oxygen, for example, there would be no way to make water, a vital ingredient in beer. --Dave Barry


--------------------
Not all chemicals are bad. Without chemicals such as hydrogen and oxygen, for example, there would be no way to make water, a vital ingredient in beer. --Dave Barry


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Offlinepj541
member
Registered: 02/04/01
Posts: 128
Loc: fuck off
Last seen: 14 years, 7 months
Re: green agar [Re: duncan8r]
    #408646 - 09/30/01 12:04 AM (15 years, 2 months ago)

thanx for the replies i transfered the most promising sectons from the 2 clean plates to 5 new plates i guess it was the print.
thanx again pj


"If you are frightened of the end and your holding on you see devils tearing your life away but if you've made your peace then the devils are really angels freeing you from the earth.."

DON'T ORDER FROM STP!!!!


--------------------
?Well, this is how the world works. All energies flow according to the whims of the great magnet. What a fool I was to defy him.?



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OfflineAzure
old hand
Registered: 01/01/99
Posts: 469
Loc: California, USA
Last seen: 14 years, 7 months
Re: green agar [Re: pj541]
    #408756 - 09/30/01 01:30 AM (15 years, 2 months ago)

It's most likely either from the spores or the tool you used to scrape the spores off the print and put it onto the dish. To determine which one it is, sterilize the tool completely with isopropyl alcohol and flame the tip before use.



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Offlinepj541
member
Registered: 02/04/01
Posts: 128
Loc: fuck off
Last seen: 14 years, 7 months
Re: green agar [Re: Azure]
    #409232 - 09/30/01 03:30 PM (15 years, 2 months ago)

I used disposable plastic loops so i could not flame sterilize and i was under the impression that alcohol distroys spores.

Does alcohol distroy spores?

The Shroomery needs help, ask Thor what you can do! This is your site too!


--------------------
?Well, this is how the world works. All energies flow according to the whims of the great magnet. What a fool I was to defy him.?



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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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