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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Woodlover Pasturization Question
    #5111246 -

I've been experimenting with a wood-loving species and would like some advice about pasteurization.

I started with some wild harvested colonized wood fragments, and so far have tried spawning them to fresh wood chips and to saw dust from wood pellet fuel, both indoors in Glad-ware plastic dishes. I did not pasteurize or sterilize in either of these experiments.

The wood chip to wood chip is colonizing nicely with no contams, but is proceeding very slowly. Lots of rhizomorphs but after two months, most of the wood chips have not turned perceptively white.

The wood-chip to pellet fuel is even slower. Dense mycelial growth forms in the moist saw dust for about half an inch around each wood chip, but then stalls. Also, the uncolonized saw dust is growing red and green molds, but I don't think this is a problem because the the myc seems to colonize moldy saw dust about as well as the non-moldy.

I'm hoping to have about ten gallons of spawn to start an outdoor bed in the Spring, and at the current rate, I will never make it.

I'm thinking of adding some well weathered horse poo to the mix to speed things up a little, and I'm wondering if pasteurization will be necessary.

I'm planing two substrates; about 5:1 wood chips to poo spawned with colonized wood chips, and 5:1 pellet fuel sawdust to poo spawned with colonized saw dust.

Would you recommend pasteurizing either or both substrates, and if so, could this be done in an aluminum pan in the oven?

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Re: Woodlover Pasturization Question [Re: shroomydan]
    #5111264 -

Mix 50 ml mollasis to 20 liters of water. Soak the chips in that for 3 or 4 days. Then sterilize in an autclave, 2 hours at 15 psi.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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Re: Woodlover Pasturization Question [Re: shroomydan]
    #5111272 -

there was a tek that involved pasteurization in the oven using those reynolds oven bags. i personally wouldn't recommend using the poo for spawn generation. when i grew out azures i used a 50/50 sawdust of hemlock and chips from a combination of beech and poplar trees and they seemed to work out good for me. maybe your moisture is off on the wood and that is why your chips only colonize partially. try soaking your chips for a day or two first.


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Re: Woodlover Pasturization Question [Re: Psychoslut]
    #5111282 -

Shroomydan,
You do realize that your spawn is used to inoculate an outdoor bed of wood chips don't you? Ten gallons of spawn seems like an awful lot considering you'll have 3-4 months for the spawn to colonize the wood chips in the outdoor bed. How big is this outdoor bed?

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Re: Woodlover Pasturization Question [Re: Wronguy]
    #5111316 -

Aye, it is not my formula, its Pauls.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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Re: Woodlover Pasturization Question [Re: Psychoslut]
    #5111323 -

Sorry about that man. I was replying to Shroomydan.

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Re: Woodlover Pasturization Question [Re: Wronguy]
    #5111335 -

There is nothing wrong with inoculating 3 tons of wood chips  :tongue:.

I actually plan to have at least 10 gallons of spawn for each of Psilocybe azurescens, and Stropharia ruggosoannalata, before spring time.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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Re: Woodlover Pasturization Question [Re: Psychoslut]
    #5111354 -

I agree that there is nothing wrong with having a ton of spawn. Shroomydan seems to be in a bit of a time crunch, so I'm just letting him know that a large amount of spawn is not required.

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Re: Woodlover Pasturization Question [Re: Wronguy]
    #5111357 -

It's about thirty square feet, and right now I only have about one quart of weakly colonized chips. It's my understanding that the chips should be well digested and white before using them to spawn more chips. Poo is easier to digest than wood, so I thought adding some might speed things up a little. :shrug:




Psychoslut, that sounds like a great idea, but I don't have an autoclave. 

I soaked the chips over night, and the moisture appears to be OK. Perhaps I should fill the colonizing container with water, let it sit for a while then drain?

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Re: Woodlover Pasturization Question [Re: Wronguy]
    #5111374 -

Yeah. Im not going to start making spawn untill early march. I plan to do 12 quart jars, and G2G each jar to 1 bag holding a gallon of sterile wood chips each. They will be ready for bed spawning in may. Do you know the typical spawning ratio to soaked chips outdoors?

Like how many gallons of chips can 1 gallon of colonized chips spawn?


--------------------



[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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Re: Woodlover Pasturization Question [Re: shroomydan]
    #5111377 -

i don't think overnight is long enough of a soak. i wait at least a day until the chips no longer float.

an in jar dunk??? sounds like some inovative thinking... i like it. how long would you dunk for. i would immagine that wood would require a 12-24 hour dunk just like any other.


--------------------
:tripping::hypno::tripping: :boobs:Patience Is Key In This Hobby.:boobs: :tripping::hypno::tripping:
Black Cherry Bud
Burmese Kush
Grapefruit X Skunk
Bubble Hash With TheNewGuy
BHO With TheNewGuy

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Re: Woodlover Pasturization Question [Re: thenewguy05]
    #5111394 -

Wood soaks up water allot slower than grain. GGMM says 2-3 days soaking.


--------------------



[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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