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OfflineFreezen
newbie
Registered: 01/22/01
Posts: 27
Last seen: 22 years, 2 months
Bad substrate, or bad steps?
    #478378 - 12/03/01 02:15 PM (22 years, 7 months ago)

OK.. Long and detailed, urgent because I'm doing more tonight.

Using birdseed.. ingredients, in order: Canary Grass Seed, White Millet, Oat Groats, Mineral Oil.
Steeped overnight, boiled for 45min, rinsed, p/c for 1 hour in (7) 1 quart jars. Used 2 MM Filter Discs on each.
Syringe purchased, not self-made. Innoculated via oven tek. 2cc per jar. Incubated in dark area, regular room humidity(30%), 75 degrees, 2 months.

1 jar had green contams within 2 weeks. 2 other jars started to show green contams within 4 weeks. Remainder of jars have yet to show any mycel growth. Birdseed seems to have a white, jelly-like substance throughout, seems to have come from within the seeds. Threw out, is indeed gel-like, non mycellium.

Is this jelly normal? Was the syringe bad? Should I use a different birdseed? Did I do something wrong without realizing? Please, please, PLEASE help!


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There are three types of people in this world:
Those who can count, and those who can't.

Edited by Freezen (12/06/01 06:29 PM)

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OfflineFreezen
newbie
Registered: 01/22/01
Posts: 27
Last seen: 22 years, 2 months
Re: Bad substrate, or bad steps? [Re: Freezen]
    #482288 - 12/06/01 06:30 PM (22 years, 7 months ago)

Reposting due to lack of response and need answers QUICK..


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There are three types of people in this world:
Those who can count, and those who can't.

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Offlineegolesss
veteran
Registered: 10/25/00
Posts: 1,005
Last seen: 21 years, 8 months
Re: Bad substrate, or bad steps? [Re: Freezen]
    #482324 - 12/06/01 07:06 PM (22 years, 7 months ago)

Did you use a pressure cooker?
I would like to see quite a bit of verm in that mix after steeping and cooking, to soak up extra moiture.
2cc per jar will add alot of moisture to a jar especially on the bottom without verm ect to absorb it..
75degrees 2 months? birdseed colonizes very fast half that time even at a low 75.....
mineral oil may be the jel-like substance try using a wild bird mix from walmart, it has less bird kote and sunflower seeds in it..... ...


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Going crazy will drive you mad, but once you get there the rest is easy....All spores are not created equal!!!!!!!!!!! Sporeworks, Hawkseye, PF, they are completely viable with very strong genetics.



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