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 liftedoff420
i need drugs
Registered: 01/17/03
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 Loading up substrate and sterilizing later? 
#1319563 - 02/19/03 02:53 PM (14 years, 5 months ago) |
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today is wednesday.....i was planning on loading up substrates into my jars today....then sterilizing on friday because that is the only day i can do it.
will the jars become contaminated within the 2 days?
advice...advice...adivce
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 Roadkill
Retired Shroomery Mod
 
Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
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 Re: Loading up substrate and sterilizing later? [Re: liftedoff420]
#1319645 - 02/19/03 03:26 PM (14 years, 5 months ago) |
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yes they will contaminate.
you don't want to wait to sterilize BRF jars.
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with  on there names, that means they have mushrooms for sale.
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soochi
Chef
Registered: 08/13/02
Posts: 2,420
Loc: The Richest County
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Re: Loading up substrate and sterilizing later? [Re: liftedoff420]
#1319646 - 02/19/03 03:26 PM (14 years, 5 months ago) |
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ive loaded jars the day before pcing and didn't have a problem, another day shouldn't hurt.
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Wi' bickering brattle!
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Psychoslut
The Mother Fucking Bear-o-dactyl
Registered: 12/10/02
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Loc: all up in ya
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Re: Loading up substrate and sterilizing later? [Re: Roadkill]
#1319677 - 02/19/03 03:36 PM (14 years, 5 months ago) |
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couldnt he just kill whatever contams pop up in the ole pcer?
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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]
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liftedoff420
i need drugs
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Re: Loading up substrate and sterilizing later? [Re: Psychoslut]
#1319777 - 02/19/03 04:08 PM (14 years, 5 months ago) |
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even if it does contaminate.....wont the bacteria be killed when i sterilize?? i have no choice other than to do this on friday....i just loaded up 8 jars with brf...watver...i will see what happens
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,598
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Re: Loading up substrate and sterilizing later? [Re: Psychoslut]
#1319826 - 02/19/03 04:21 PM (14 years, 5 months ago) |
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Quote:
couldnt he just kill whatever contams pop up in the ole pcer?
Nope
I'll let Anno explain this one...he's much better at it than me.
I did a test...I left 4 jars out...not sterilized.
Within 2 to 3 days they got contaminated.
Do what you want!
I wouldn't do it!
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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DrJoseph
Wendy shot thegun.
Registered: 10/03/01
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Re: Loading up substrate and sterilizing later? [Re: liftedoff420]
#1319835 - 02/19/03 04:24 PM (14 years, 5 months ago) |
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load up the jars minus the water.. just add the water before you sterilize.
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"cus it aint gonna be like you think it is - thats the one thing you can take to the bank." - Terance mckenna
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liftedoff420
i need drugs
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Re: Loading up substrate and sterilizing later? [Re: DrJoseph]
#1319862 - 02/19/03 04:34 PM (14 years, 5 months ago) |
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fuck too late...o well....i'll see how many jars survive....its wednesday night, and im sterilizing on friday afternoon, if one or two get lost to contam it wont really matter i still got a shitload of verm and brf
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deanofmean
mycophagous
Registered: 12/07/02
Posts: 2,017
Loc: PNW
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 Re: Loading up substrate and sterilizing later? [Re: liftedoff420]
#1319910 - 02/19/03 04:48 PM (14 years, 5 months ago) |
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a waste of spores . 
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
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Re: Loading up substrate and sterilizing later? [Re: DrJoseph]
#1319918 - 02/19/03 04:51 PM (14 years, 5 months ago) |
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Quote:
load up the jars minus the water.. just add the water before you sterilize.
huh?
that makes no sense whats so ever!!
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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SHR00M0L0GIST
Psilocybin Test Subject
Registered: 11/27/02
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Re: Loading up substrate and sterilizing later? [Re: Roadkill]
#1320154 - 02/19/03 06:11 PM (14 years, 5 months ago) |
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Why didn't you just wait till you had a day to do it all at once? That makes a lot more sense to me. The best thing you can do is be patient and take your time when cultivating mushrooms. Don't rush or half-ass anything. The whole process already takes a good amount of time. Why set yourself back with a mistake when you can just do it the right way the first time? I always take my time and I am yet to have a contamination problem.
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liftedoff420
i need drugs
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Re: Loading up substrate and sterilizing later? [Re: SHR00M0L0GIST]
#1320177 - 02/19/03 06:18 PM (14 years, 5 months ago) |
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basically i have absolutely no privacy in my house, with my dad out of work at home all the time...so i have to go to a friends house to sterilize....if the jars get contaminated i will just wip up another batch at my friends
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SHR00M0L0GIST
Psilocybin Test Subject
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Re: Loading up substrate and sterilizing later? [Re: liftedoff420]
#1320219 - 02/19/03 06:29 PM (14 years, 5 months ago) |
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Oh ok. Well, Good Luck Man! You should be ok.
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blackout
Microwaver
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Re: Loading up substrate and sterilizing later? [Re: Roadkill]
#1320720 - 02/20/03 12:04 AM (14 years, 5 months ago) |
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"I did a test...I left 4 jars out...not sterilized.
Within 2 to 3 days they got contaminated"
i would have thought if you left them in a cold place they shouldnt go bad in 2-3 days. were they hot? were there very obvious signs of mould.
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Anno
Experimenter
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Re: Loading up substrate and sterilizing later? [Re: liftedoff420]
#1320732 - 02/20/03 12:21 AM (14 years, 5 months ago) |
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>I'll let Anno explain this one...he's much better at it than me.
Hmmm...
The best method is to prepare the jars, and PC right away.
If you leave the substrate non.sterilized, it will eventually contaminate.
The time until it actually visibly and smellibly  contaminates will depend on many, many factors: the temperature, the kind of substrate, the amoount of contaminats in your room, grain, vermiculite, tools, the kind of those cpontaminats....
It can take 3 days, but it can take only 12 hours, depends.
A sterilization(as we perform it) is never complete.
There is always a certain percentage of contaminants that survives.
The more contaminants there are initially, the more will survive the sterilization process.
The second reason why this is bad, is that the contaminants produce all sorts of potentially hazardous substances. Even if you PC the jars and manage to kill the contaminants completely, the substances will still remain in the substrate and might eventually end up in your shrooms.
Edited by Anno (02/20/03 02:25 AM)
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mike
iHugTrees
Registered: 01/14/03
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Re: Loading up substrate and sterilizing later? [Re: Anno]
#1320805 - 02/20/03 02:11 AM (14 years, 5 months ago) |
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sorry man, i accidently left one jar for 3 days, and you can guess what happened, contams, big smelly ones.
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Once you can accept the universe as matter expanding into nothing that is something, wearing stripes with plaid comes easy.
-Albert Einstein
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blackout
Microwaver
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Re: Loading up substrate and sterilizing later? [Re: mike]
#1320897 - 02/20/03 04:06 AM (14 years, 5 months ago) |
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"The second reason why this is bad, is that the contaminants produce all sorts of potentially hazardous substances. Even if you PC the jars and manage to kill the contaminants completely, the substances will still remain in the substrate and might eventually end up in your shrooms."
this would be especially important when growing sclerotia
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