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OfflineAnnoA
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A. bisporus culture contaminant
    #475650 - 12/01/01 04:05 AM (15 years, 6 months ago)

I cloned a button mushroom from the supermarket 3 months ago. It needed time to begin growing on agar, so it took 2 months for the dish to colonize. Then I saw that a different mycelium began to grow from the edge of the dish, and now after app. a month here is the result.
The mycelium looks loke cubensis, but the shrooms don?t quite.
What is it?



A bigger picture can be found here:

http://annonymer.members.easyspace.com/Ab_contaminate.jpg


Edited by Anno (12/01/01 11:33 AM)


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Offlinestefan
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Re: A. bisporus culture contaminant [Re: Anno]
    #475712 - 12/01/01 05:13 AM (15 years, 6 months ago)

hmmmm interesting, maybe you accidentally created a whole new mutant mushroom race!
maybe these are psychoactive button mushrooms...lol
btw I thought micelia of two different species couldn't live together, at least mot mixing. right? I thought that the strongest mycelium would be the only one surviving.


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Anonymous

Re: A. bisporus culture contaminant [Re: Anno]
    #475740 - 12/01/01 05:40 AM (15 years, 6 months ago)

I'm not seeing anything abnormal there... cept the agar appears to have dried up a bit, probably from sitting for 2 months.. turning the mycel into shredded cotton. I've seen that happen

I dont do much agar work though, so i'm sure the next response will be more informative


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OfflineAnnoA
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Re: A. bisporus culture contaminant [Re: Anonymous]
    #475746 - 12/01/01 05:47 AM (15 years, 6 months ago)

>I'm not seeing anything abnormal there...

Hmm.... Don?t you see the mushrooms growing? They are supoosed to be white button mushrooms.....


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Re: A. bisporus culture contaminant [Re: Anno]
    #475766 - 12/01/01 06:22 AM (15 years, 6 months ago)

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Offlinestefan
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Re: A. bisporus culture contaminant [Re: Anno]
    #475782 - 12/01/01 06:52 AM (15 years, 6 months ago)

Maybe you didn't clean the petridish good enough from earlier cubensis cultures. Or a lonely cubensis spore accidentally fell into the agar.


Edited by stefan (12/01/01 06:53 AM)


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OfflineDinoMyc
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Re: A. bisporus culture contaminant [Re: Anno]
    #476095 - 12/01/01 03:08 PM (15 years, 6 months ago)

looks like contam from all the spores you have from other endevours.. remember, all it really takes is two spores..


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Anonymous

Re: A. bisporus culture contaminant [Re: Anno]
    #476115 - 12/01/01 03:47 PM (15 years, 6 months ago)

this is what you wrote anno:
>The mycelium looks like cubensis, but the shrooms don?t quite

But yea, you are right... they both look like bensis, the mycelium and the friuties. if you used agaricus on that plate then that is truly a strange phenomena, grow it out and find out what it really is, now ya got me curious.


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InvisibleFloydster
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Re: A. bisporus culture contaminant [Re: Anno]
    #476126 - 12/01/01 03:59 PM (15 years, 6 months ago)

looks like cubies to me, not that ive ever actually seen one...iwould say some stray spores found their way into dishes. seems like a good strain too. strong and resistant to that supermarket mushrooms mycelium.


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InvisibleCompilerV
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Post deleted by Administrator [Re: Anno]
    #476612 - 12/02/01 01:04 AM (15 years, 6 months ago)



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Offlinetrade omlet
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Re: A. bisporus culture contaminant [Re: Compiler]
    #476855 - 12/02/01 05:56 AM (15 years, 6 months ago)

Hi anno.
This looks very interesting. To me the mushrooms look like a Psilocybe for sure. Actually looks like a grass loving psilocybe in its early stages. But pinning resembles the heavy pinning of cubensis on agar. Dont know what do make of it.
Try to grow it out on grain, and see whats up. Looks a bit like mexicana... Have you had mexicana in that plate?


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OfflineAnnoA
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Re: A. bisporus culture contaminant [Re: trade omlet]
    #476863 - 12/02/01 06:19 AM (15 years, 6 months ago)

Well, it?s growing fast.



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OfflineB.I.O.
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Re: A. bisporus culture contaminant [Re: Compiler]
    #476933 - 12/02/01 10:38 AM (15 years, 6 months ago)

anno...that on the left seems to be an agaricus mycelium...the other with the pins looks like cubies...
i think you have 2 strains on that petri...the mycelium right is
far too rhizomorh...the other is more tomentose what resembles more to agaricus species....if you need agaricus prints, i have a few...

BiO.


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OfflineAnnoA
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Re: A. bisporus culture contaminant [Re: B.I.O.]
    #577733 - 03/13/02 11:01 AM (15 years, 3 months ago)

I isolated the rhyzomorph mycelium and fruited it on birdseed.
Here?s the result, second flush:



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OfflineSterile
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Re: A. bisporus culture contaminant [Re: Anno]
    #577751 - 03/13/02 11:21 AM (15 years, 3 months ago)

Holy shit! Is this shroom very big, or is my monitor too small? lol Looks like you have been growing too many Psilos in there Anno, and they started learning how to grow alone, LOL


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OfflineAnnoA
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Re: A. bisporus culture contaminant [Re: Sterile]
    #577755 - 03/13/02 11:24 AM (15 years, 3 months ago)

Hehe, I learned how to photograph :wink: .


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OfflineHumboldtHort
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Re: A. bisporus culture contaminant [Re: Anno]
    #577805 - 03/13/02 12:24 PM (15 years, 3 months ago)

Did you id that mushroom? What species did it key out as?


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OfflineSeussA
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Re: A. bisporus culture contaminant [Re: Anno]
    #577813 - 03/13/02 12:38 PM (15 years, 3 months ago)

Any idea where the contamination came from? (I never thought I would call cubensis a contaminate.) Was it on the original button you cloned, or did it get introduced later?


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OfflineAnnoA
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Re: A. bisporus culture contaminant [Re: HumboldtHort]
    #577818 - 03/13/02 12:50 PM (15 years, 3 months ago)

Well, it?s definitely a P. cubensis.

Seuss: I don?t really know. Perhaps some spores fell on the agar during the inoculation.
I had a flow hood to that time already, but who knows....


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