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 Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 6 days, 11 hours
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 A. bisporus culture contaminant 
#475650 - 12/01/01 02:05 AM (22 years, 8 days ago) |
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I cloned a button mushroom from the supermarket 3 months ago. It needed time to begin growing on agar, so it took 2 months for the dish to colonize. Then I saw that a different mycelium began to grow from the edge of the dish, and now after app. a month here is the result.
The mycelium looks loke cubensis, but the shrooms don?t quite.
What is it?
A bigger picture can be found here:
http://annonymer.members.easyspace.com/Ab_contaminate.jpg
Edited by Anno (12/01/01 09:33 AM)
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stefan
work in progress
Registered: 04/11/01
Posts: 8,932
Loc: The Netherlands
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 Re: A. bisporus culture contaminant [Re: Anno]
#475712 - 12/01/01 03:13 AM (22 years, 8 days ago) |
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hmmmm interesting, maybe you accidentally created a whole new mutant mushroom race!
maybe these are psychoactive button mushrooms...lol
btw I thought micelia of two different species couldn't live together, at least mot mixing. right? I thought that the strongest mycelium would be the only one surviving.
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Anonymous
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Re: A. bisporus culture contaminant [Re: Anno]
#475740 - 12/01/01 03:40 AM (22 years, 8 days ago) |
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I'm not seeing anything abnormal there... cept the agar appears to have dried up a bit, probably from sitting for 2 months.. turning the mycel into shredded cotton. I've seen that happen
I dont do much agar work though, so i'm sure the next response will be more informative
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 6 days, 11 hours
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Re: A. bisporus culture contaminant [Re: Anonymous]
#475746 - 12/01/01 03:47 AM (22 years, 8 days ago) |
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>I'm not seeing anything abnormal there...
Hmm.... Don?t you see the mushrooms growing? They are supoosed to be white button mushrooms.....
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 World Spirit
PNW
Registered: 07/27/01
Posts: 9,817
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Re: A. bisporus culture contaminant [Re: Anno]
#475766 - 12/01/01 04:22 AM (22 years, 8 days ago) |
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Deleted by admin
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stefan
work in progress
Registered: 04/11/01
Posts: 8,932
Loc: The Netherlands
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Re: A. bisporus culture contaminant [Re: Anno]
#475782 - 12/01/01 04:52 AM (22 years, 8 days ago) |
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Maybe you didn't clean the petridish good enough from earlier cubensis cultures. Or a lonely cubensis spore accidentally fell into the agar.
Edited by stefan (12/01/01 04:53 AM)
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DinoMyc
Ipsa scientiapotestas est
Registered: 11/13/99
Posts: 1,080
Last seen: 17 years, 5 months
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Re: A. bisporus culture contaminant [Re: Anno]
#476095 - 12/01/01 01:08 PM (22 years, 8 days ago) |
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looks like contam from all the spores you have from other endevours.. remember, all it really takes is two spores..
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I merely came to listen, came to say.
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Anonymous
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Re: A. bisporus culture contaminant [Re: Anno]
#476115 - 12/01/01 01:47 PM (22 years, 8 days ago) |
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this is what you wrote anno:
>The mycelium looks like cubensis, but the shrooms don?t quite
But yea, you are right... they both look like bensis, the mycelium and the friuties. if you used agaricus on that plate then that is truly a strange phenomena, grow it out and find out what it really is, now ya got me curious.
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Floydster
journeyman
Registered: 03/30/01
Posts: 97
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Re: A. bisporus culture contaminant [Re: Anno]
#476126 - 12/01/01 01:59 PM (22 years, 8 days ago) |
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looks like cubies to me, not that ive ever actually seen one...iwould say some stray spores found their way into dishes. seems like a good strain too. strong and resistant to that supermarket mushrooms mycelium.
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Compiler
Registered: 11/03/01
Posts: 162
Loc: USA
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Post deleted by Administrator [Re: Anno]
#476612 - 12/01/01 11:04 PM (22 years, 8 days ago) |
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Compiler
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trade omlet
member
Registered: 04/05/00
Posts: 35
Last seen: 21 years, 7 months
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 Re: A. bisporus culture contaminant [Re: Compiler]
#476855 - 12/02/01 03:56 AM (22 years, 7 days ago) |
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Hi anno.
This looks very interesting. To me the mushrooms look like a Psilocybe for sure. Actually looks like a grass loving psilocybe in its early stages. But pinning resembles the heavy pinning of cubensis on agar. Dont know what do make of it.
Try to grow it out on grain, and see whats up. Looks a bit like mexicana... Have you had mexicana in that plate?
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
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Last seen: 6 days, 11 hours
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Re: A. bisporus culture contaminant [Re: trade omlet]
#476863 - 12/02/01 04:19 AM (22 years, 7 days ago) |
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Well, it?s growing fast.
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B.I.O.
old hand
Registered: 05/26/99
Posts: 458
Last seen: 9 months, 6 days
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Re: A. bisporus culture contaminant [Re: Compiler]
#476933 - 12/02/01 08:38 AM (22 years, 7 days ago) |
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anno...that on the left seems to be an agaricus mycelium...the other with the pins looks like cubies...
i think you have 2 strains on that petri...the mycelium right is
far too rhizomorh...the other is more tomentose what resembles more to agaricus species....if you need agaricus prints, i have a few...
BiO.
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 6 days, 11 hours
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Re: A. bisporus culture contaminant [Re: B.I.O.]
#577733 - 03/13/02 09:01 AM (21 years, 8 months ago) |
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I isolated the rhyzomorph mycelium and fruited it on birdseed.
Here?s the result, second flush:
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Sterile
mushroom lover
Registered: 03/16/01
Posts: 2,535
Loc: under the Amanita
Last seen: 2 months, 16 days
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Re: A. bisporus culture contaminant [Re: Anno]
#577751 - 03/13/02 09:21 AM (21 years, 8 months ago) |
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Holy shit! Is this shroom very big, or is my monitor too small? lol Looks like you have been growing too many Psilos in there Anno, and they started learning how to grow alone, LOL
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The Source Of The Force
Is The Power Of The Mind
"if you don't like what you're doing, you can always pick up your needle and move to another groove." - timothy leary"
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 6 days, 11 hours
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Re: A. bisporus culture contaminant [Re: Sterile]
#577755 - 03/13/02 09:24 AM (21 years, 8 months ago) |
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Hehe, I learned how to photograph  .
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HumboldtHort
newbie
Registered: 02/13/02
Posts: 40
Last seen: 19 years, 8 months
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Re: A. bisporus culture contaminant [Re: Anno]
#577805 - 03/13/02 10:24 AM (21 years, 8 months ago) |
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Did you id that mushroom? What species did it key out as?
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A gram is better than a damn
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Seuss
Error: divide byzero
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
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Re: A. bisporus culture contaminant [Re: Anno]
#577813 - 03/13/02 10:38 AM (21 years, 8 months ago) |
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Any idea where the contamination came from? (I never thought I would call cubensis a contaminate.) Was it on the original button you cloned, or did it get introduced later?
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Just another spore in the wind.
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 6 days, 11 hours
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Re: A. bisporus culture contaminant [Re: HumboldtHort]
#577818 - 03/13/02 10:50 AM (21 years, 8 months ago) |
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Well, it?s definitely a P. cubensis.
Seuss: I don?t really know. Perhaps some spores fell on the agar during the inoculation.
I had a flow hood to that time already, but who knows....
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