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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Spore mass slurry * 1
    #4642913 -

Has anyone tried this from the "natural cultivation" chapter in GGMM?

"For those wishing to begin a mushroom patch
using fresh specimens, a more efficient method
of spore collection is recommended. This
method calls for the immersion of the mushroom
in water to create a spore mass slurry. Choose
fairly mature mushrooms and submerge them in
a 5 gallon bucket ofwater. A gram or two of table
salt inhibits bacteria from growing while not substantially
affecting the viability of the spores. By
adding 50 ml. of molasses spores are stimulated
into frenzied germination. After four hours of
soaking, remove the mushroom(s) from the
bucket. Most mushrooms will have released tens
of thousands of spores. Allow the broth to sit for
24-48 hours at a temperature above 50? E (10? C.)
but under 80? F. (27? C.) In most cases, spores
begin to germinate in minutes to hours, aggressively
in search of new mates and nutrients. This
slurry can be expanded by a factor of ten in 48
hours. (I have often dreamed, being the mad scientist,
of using spore mass slurries of Morels and
other species to aerially "bomb " large expanses
of forest lands. This idea, as crazy as it may initially
sound, warrants serious investigation.)
During this stage of frenzied spore germination,
the mushroom patch habitat should be
designed and constructed. Each species has
unique requirements for substrate components
for fruiting. However, mycelia of most species
will run through a variety of lignin-cellulosic
wastes. Only at the stage when fruitbody production
is sought does the precise formulation of the
substrate become crucial."

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Re: Spore mass slurry [Re: ambros]
    #4643040 -

really interesting, thanks for sharing that!

it's an un-sterile liquid culture. probably work well outdoors or for colonizing bulk poop.

that guy really is a mad scientist -- mycelia bombs (LOL)

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Re: Spore mass slurry [Re: puwtrip]
    #4643075 -

The "natural cultivation" chapter is all abut unsterile outdoor cultivation.

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Re: Spore mass slurry [Re: ambros]
    #4644493 -

All I need is a helicopter...  :laugh:

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Re: Spore mass slurry [Re: Land_Crab]
    #4647605 -

Stamets actually DID make this dream come true. I remember many years back seeing the threads in the alt. groups where he requested assistance in putting this together. He donated 1000 spawn bas, found a plane/pilot and did do the aerial bombing of the morel mycelia on a burn area (either created or natural, I can't remember...)
Unfortunately, I also heard that there was no fruiting of morels to report. But they're definitely the elite of elusivity, and you never know how, what, when , or why. If your interested, go to Google Groups and you can do a search (he posted under stamets1@aol.com).
Light and Love,
Jeremy Davis


--------------------
Jeremy Davis
Educational Concerns for Hunger Organization, Inc.
Check out the ECHO mushroom blog page to see our lab, growing facility, and more-www.echotech.org/greta

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Re: Spore mass slurry [Re: Jeremy_Davis]
    #4649172 -

What interests me too is the tolerance to salt. I was going to try out cold sterilization of grains, soaking them in water then injecting bleach 2-3 days later when the endospores have germinated, the bleach will degrade into salt and chlorine gas.
Seems it may be viable to do a large LC the same way.

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Re: Spore mass slurry [Re: ambros]
    #4668720 -

Ive used this tek several times

For twenty years my Dad had a large pile of wood debris in the middle of a large open back field. Over the years the pile kept getting bigger with braches from trees that fell during storms, winter, yard clipping, hedge clipping etc.
I first used this tek several years ago one spring, using Oyster mushrooms, made five, five gallon spore mass slury buckets and dumped all over this huge pile of debris. It worked great! About six weeks after soaking this pile with the slury, hundreds of mushrooms grew.
Later that year after the shrooms had rotted away, all kinds of new growth took over. The shrooms rotted and atracted flies and other insects which atracted birds, which brought seeds. Today, several years later, the debris pile is gone, replaced by an oasis of life.
The wood debris has been recycled into soil, there are several species of trees, flowers, wild grasses, blueberries, raspberries, juniper bushes in its place.

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Re: Spore mass slurry [Re: noxy]
    #4668824 -

Noxy, your wording sounded incredibly familiar, and it was bugging me to know if I'd read it from you before or not. I realized it was from stamets page on mycoremediation (http://www.fungi.com/mycotech/mycova.html). Listen to this:
Quote:
After 8 weeks, the mushrooms had rotted away, and then came another startling revelation. As the mushrooms rotted, flies were attracted. (Sciarid, Phorid and other "fungus gnats" commonly seek out mushrooms, engorged themselves with spores, and spread the spores to other habitats). The flies became a magnet for other insects, which in turn brought in birds. Apparently the birds brought in seeds. Soon ours was an oasis, the only pile teeming with life!


sound familiar?


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: Spore mass slurry [Re: mycofile]
    #4668868 -

Im very familier with Stamets work, I didnt quote the man, but how else can one explain the transformation without using some of the same words? "an oasis of life" theres no other way to explain it.

Bioremediation is a facinating field, and I am curently engaged in research and development in that field, relying heavily on Stamets work as a point of origin, so to speak.

But the point is, its a true story, and if it reeks of Stametism, it is only because the procedure is based on Stamets work, it was not my intention to "steal" any ones words or ideas

The familiar thing is the process, and it belongs to Mother Nature, mushrooms attract flies, flies attract birds, birds bring seeds
This process is the same every time. Its how nature responds to catastrophi, weather it be a volcano, or fire, flood or landslide,
mushrooms will move in and begin the natural process of remediation, recycling waste, refuse and contaminats back into the soil

It works, and the results are VERY repeatable
so repeatable in fact, I dont think Paul will mind.
Except for the fact that he has a patent on the idea of using a mycellial mat for bioremediation. Personally I dont think you should be able to patent a natural process, and I let him know that every chance I can. Did you hear that Paul?

Edited by noxy (09/16/05 10:02 AM)

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Re: Spore mass slurry [Re: noxy]
    #4670709 -

Oh, I wasn't trying to knock you or accuse you of plagarism or anything. i just wondered if you had read that description or not.

Bio-remediation is dope. i also think stamets shouldn't patent the process, or if he does it should be an open-use patent, just so some other corp can't patent it and control it.

I'm also working on cleaning up petroleum contaminated land. It takes a long time and a lot of work to move the soil into berms. I wish it were as easy as using a spore mass slurry of some mycorizah, and planting a meadow! (well, it may be, but go with what works unless you have the backing to persue a new patent, you know?).


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: Spore mass slurry [Re: mycofile]
    #4671306 -

Thats something to think about

also, going back to the lab and taking a second look at all those strains selected out of the process of finding a pure fruiting strain,
and see how well some of those pure-vegative strains can break down hydrocarbons.
As a science, we know so little about mushrooms, and there could be
thousands of species yet to be discovered

There has got to be a giant active psilocybe growing somewhere
maby somewhere in Africa
why not? big 22 to 24 inch spore print
scatered to gregarious on large piles of elephant dung
that would be cool

Edited by noxy (09/16/05 08:34 PM)

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Re: Spore mass slurry [Re: noxy]
    #4718225 -

Hey noxy, thoroughly off-subject here, but just wanted to give you a heads up on stamets new book. It's apparently focused on myco-remediation, myco-filtration, myco-forestry and myco-gardening. Sounds right up our alley, eh?


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: Spore mass slurry [Re: ambros]
    #16093213 -

Thanks for the post.  Going to see Paul in the Fall.

How many Mushies for 5 gal slurry do you recommend?  I am thinking the more the merrier right?

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Re: Spore mass slurry [Re: Tilzungulous]
    #16094812 -

it's an un-sterile liquid culture. probably work well outdoors or for colonizing bulk poop.

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Re: Spore mass slurry [Re: ambros]
    #22455969 -

ambros said:
Most species
will run through a variety of lignin-cellulosic
wastes. Only at the stage when fruitbody production
is sought does the precise formulation of the
substrate become crucial."


What does that mean in laymen terms? I want to put the slurry in a super soaker and shoot around.


--------------------
)))))))))))))))))0000000ooo_{}_ooo0000000(((((((((((((((((

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Re: Spore mass slurry [Re: ShapungGold]
    #22456305 -

fungi likes dead plant stuff

but substrate is more important when fruiting

(except laccases are correlated strongly w/ fruiting) <= this was my addition, feel free to ignore


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)

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