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my neighbor's cat has been trying to get me interested in honey-water teks. it seems that there are many difficulties for a cat to do the involved proceedures and sterile teks.
1. on most attempts, we got jars of sterilized honey water (that for experimental reasons varied from almost clear to amber-honey colored.) that had floaties in them the floaties look like white to amber colored pond scum. i switched to using distilled water and more carefull to not get too hot during pressure cooking, but still get considerable floaties. are the floaties okay, and what the hell are they?
2. when attempting to get spores to germinate, i cannot tell an innoculated jar from one that is not, ten days after, despite repeated attempts and care not to kill the spores. tested the syringe and some growth is evident, though not spectacular. is there a definute way to tell if and how well a jar is growing with mycelium?
3. attempted cloning. used fresh vibrant desireable PF house strain specimen. soaked in H2O2 for one minute. placed in quart mason jar fitted with base of oster blender which happens to be a perfect fit. all sterile. the jar had 1/3 quart of prepared honeywater in it. blended. after several days, the bluish pieces had coelesced into a gel. reblended. waited three days. shook. attempted to innocculate jars, but has been three days and see no growth. jars were both Pf style cakes and sterilzed wheat. Any suggestions?
With the honey water tek, make sure the honey doesn't carbonize in the process. What you want to end up with is a water with less amber color to it than a crappy american beer. After innoculation, and I suggest the ramsey-seal to be employed, wait around 7-10 days. You should have what looks like small clusters of whiteish fluf. Mine almost looked like wax balls. The longer you can wait without it going bad, the more developed the mycelium will be. You'll know if you did it right, because when you innoculate jars of substrate it won't take long for you to tell if it's done correctly or not. Also, if you're using your own spores to make the honey-myco, let them rehydrate for at least 24 hours.
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