Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Advanced Mycology
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Agar transfer
    #4152797 -

when doing an agar to agar transfer to isolate a single strand. when you cut out the chunk of agar should youplace it on the petri dish mycellium facing away from the new agar medium or sandwich it with the agar? i sandwiched it basically and instead of growin out it is growing through the initial wedge first.


--------------------
Check out the official MushroomWiki at http://www.mushroomwiki.com. Stop by and contribute.

Extras: Filter Print Post Top
Re: Agar transfer [Re: xburn]
    #4152964 -

When making a transfer, I take a piece of mycelium no larger than a grain of rice, then place it myclium up on the new plate. It will grow down the sides of the original piece of agar, then into the new dish. An alternative method is to not take any agar from the first dish at all. Simply use your scalpel or inoculating loop to scrape a small piece of mycelium, then deposit it on the new dish. If you're doing strain isolations, be sure to take as small a piece as you possibly can.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
Re: Agar transfer [Re: RogerRabbit]
    #4154873 -

you will also notice that the mycelium is growing in and on top of the agar. When you cut out a section you will notice the mycelium that is inside the agar will recover first and there will be wispy strands coming out the sides.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

Extras: Filter Print Post Top
Re: Agar transfer [Re: tahoe]
    #4155265 -

ok so its just a matter of time


--------------------
Check out the official MushroomWiki at http://www.mushroomwiki.com. Stop by and contribute.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag

Mushrooms, Mycology and Psychedelics > Advanced Mycology
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* agar transfers
( 1 2 all )		 thisone 3,611 22 11/24/03 01:33 PM
by sinoptiK
* Agar Transfers CultyVader 2,551 12 04/10/03 09:44 PM
by CultyVader
* Agar transfer question Dex_Detrimental 1,189 3 06/12/02 05:51 PM
by Dex_Detrimental
* Agar: Isolation from molds... ATWAR 7,503 13 07/23/04 10:27 PM
by ATWAR
* Agar help homebrew 915 2 01/08/02 12:11 AM
by homebrew
* agar ???? ChromeCrow 918 6 05/13/02 07:30 AM
by johnwsmoke
* Agar Strain Isolation - Is it worth it?
( 1 2 all )		 Blue Helix 13,949 20 02/23/05 01:07 AM
by Blue Helix
* Is it necessary to dry agar plates? shymanta 14,975 19 07/07/15 08:59 PM
by Demonsmoke

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta
1,767 topic views. 0 members, 8 guests and 1 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.004 seconds on 14 queries.