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Amazon Shop for: Agar

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OfflineChromeCrow
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agar ????
    #628742 - 05/13/02 07:57 AM (14 years, 6 months ago)

.
CC


Edited by ChromeCrow (08/24/02 06:30 PM)


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Offlineellisdee625
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Re: agar ???? [Re: ChromeCrow]
    #628752 - 05/13/02 08:03 AM (14 years, 6 months ago)

yeah, i transfer the chunk where the most rhizomorphic growth is occurring.after 3 or 4 transfers like that, yu will notice a difference and probably be ready to innoculate with it.


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Offlinejohnwsmoke
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Re: agar ???? [Re: ChromeCrow]
    #628754 - 05/13/02 08:06 AM (14 years, 6 months ago)

Is this a tissue culture? Spore innoc? Since you have a culture started I'd first suggest that you switch to H2O2 agar. Transfer a few(or many) wedges. After adjusting to the perox. the cultures will grow wel.

If this is a spore innoc. then it is understandable that there is no rhizo/ropey growth. Also, I've had cultures that take a little while to show the desired characteristics. I'm sure you're doing fine.

JWS


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OfflineChromeCrow
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Re: agar ???? [Re: johnwsmoke]
    #628771 - 05/13/02 08:25 AM (14 years, 6 months ago)

thanks for the info, this is spore innoc, on peroxide agar. i took a small square of cardboard, nuked it in a small container of distilled water, put spore from the print on 1 side, and placed it on the per agar, spores up. lol per agar plates is all i had available, thats why i did it this way


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Offlinevatoloco
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Post deleted by MOE THE MAD SCIENTIST [Re: ChromeCrow]
    #628786 - 05/13/02 08:42 AM (14 years, 6 months ago)



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OfflineChromeCrow
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Re: agar ???? [Re: vatoloco]
    #628791 - 05/13/02 08:45 AM (14 years, 6 months ago)

.


Edited by ChromeCrow (08/24/02 06:31 PM)


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Offlinejohnwsmoke
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Re: agar ???? [Re: ChromeCrow]
    #628830 - 05/13/02 09:30 AM (14 years, 6 months ago)

Way to go CC!!! That's the type of experimentation I like to see!

I think that when you transfer you will see the quality growth you seek!!!!

Right ON!

JWS


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Jon was a crippled, midget, lezbian boy- but he stood 10' tall with a KNIFE...


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Amazon Shop for: Agar

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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