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OfflineChromeCrow
one ancient mutha
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Folding@home Statistics
Registered: 02/21/02 Happy 22nd Shroomiversary!
Posts: 1,887
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agar ????
    #628742 - 05/13/02 05:57 AM (21 years, 9 months ago)

.
CC


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ISO: Orissa, Malabar, z strain


Edited by ChromeCrow (08/24/02 04:30 PM)


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Offlineellisdee625
enthusiast
Registered: 03/27/02
Posts: 271
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Re: agar ???? [Re: ChromeCrow]
    #628752 - 05/13/02 06:03 AM (21 years, 9 months ago)

yeah, i transfer the chunk where the most rhizomorphic growth is occurring.after 3 or 4 transfers like that, yu will notice a difference and probably be ready to innoculate with it.


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Have a smurfy day!!!


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Offlinejohnwsmoke
member
Registered: 03/17/02
Posts: 104
Last seen: 13 years, 1 month
Re: agar ???? [Re: ChromeCrow]
    #628754 - 05/13/02 06:06 AM (21 years, 9 months ago)

Is this a tissue culture? Spore innoc? Since you have a culture started I'd first suggest that you switch to H2O2 agar. Transfer a few(or many) wedges. After adjusting to the perox. the cultures will grow wel.

If this is a spore innoc. then it is understandable that there is no rhizo/ropey growth. Also, I've had cultures that take a little while to show the desired characteristics. I'm sure you're doing fine.

JWS


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Jon was a crippled, midget, lezbian boy- but he stood 10' tall with a KNIFE...


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OfflineChromeCrow
one ancient mutha
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Folding@home Statistics
Registered: 02/21/02 Happy 22nd Shroomiversary!
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Re: agar ???? [Re: johnwsmoke]
    #628771 - 05/13/02 06:25 AM (21 years, 9 months ago)

thanks for the info, this is spore innoc, on peroxide agar. i took a small square of cardboard, nuked it in a small container of distilled water, put spore from the print on 1 side, and placed it on the per agar, spores up. lol per agar plates is all i had available, thats why i did it this way


--------------------
ISO: Orissa, Malabar, z strain


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Offlinevatoloco
Puppet Hunter -DBK
Registered: 01/30/02
Posts: 7,653
Last seen: 19 years, 5 months
Post deleted by MOE THE MAD SCIENTIST [Re: ChromeCrow]
    #628786 - 05/13/02 06:42 AM (21 years, 9 months ago)



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OfflineChromeCrow
one ancient mutha
Male User Gallery

Folding@home Statistics
Registered: 02/21/02 Happy 22nd Shroomiversary!
Posts: 1,887
Loc: Hoosier HELL
Last seen: 10 months, 8 days
Re: agar ???? [Re: vatoloco]
    #628791 - 05/13/02 06:45 AM (21 years, 9 months ago)

.


--------------------
ISO: Orissa, Malabar, z strain


Edited by ChromeCrow (08/24/02 04:31 PM)


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Offlinejohnwsmoke
member
Registered: 03/17/02
Posts: 104
Last seen: 13 years, 1 month
Re: agar ???? [Re: ChromeCrow]
    #628830 - 05/13/02 07:30 AM (21 years, 9 months ago)

Way to go CC!!! That's the type of experimentation I like to see!

I think that when you transfer you will see the quality growth you seek!!!!

Right ON!

JWS


--------------------
Jon was a crippled, midget, lezbian boy- but he stood 10' tall with a KNIFE...


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