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 ChromeCrow
one ancient mutha
 
Registered: 02/21/02
Posts: 1,878
Loc: Hoosier HELL
Last seen: 1 year, 7 months
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 agar ???? 
#628742 - 05/13/02 07:57 AM (15 years, 11 months ago) |
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CC
Edited by ChromeCrow (08/24/02 06:30 PM)
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ellisdee625
enthusiast
Registered: 03/28/02
Posts: 271
Last seen: 15 years, 4 months
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yeah, i transfer the chunk where the most rhizomorphic growth is occurring.after 3 or 4 transfers like that, yu will notice a difference and probably be ready to innoculate with it.
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Have a smurfy day!!!
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johnwsmoke
member
Registered: 03/17/02
Posts: 104
Last seen: 7 years, 2 months
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Is this a tissue culture? Spore innoc? Since you have a culture started I'd first suggest that you switch to H2O2 agar. Transfer a few(or many) wedges. After adjusting to the perox. the cultures will grow wel.
If this is a spore innoc. then it is understandable that there is no rhizo/ropey growth. Also, I've had cultures that take a little while to show the desired characteristics. I'm sure you're doing fine.
JWS 
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Jon was a crippled, midget, lezbian boy- but he stood 10' tall with a KNIFE...
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ChromeCrow
one ancient mutha
Registered: 02/21/02
Posts: 1,878
Loc: Hoosier HELL
Last seen: 1 year, 7 months
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thanks for the info, this is spore innoc, on peroxide agar. i took a small square of cardboard, nuked it in a small container of distilled water, put spore from the print on 1 side, and placed it on the per agar, spores up. lol per agar plates is all i had available, thats why i did it this way
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vatoloco
Puppet Hunter -DBK
Registered: 01/30/02
Posts: 7,653
Last seen: 13 years, 6 months
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 Post deleted by MOE THE MAD SCIENTIST [Re: ChromeCrow]
#628786 - 05/13/02 08:42 AM (15 years, 11 months ago) |
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ChromeCrow
one ancient mutha
Registered: 02/21/02
Posts: 1,878
Loc: Hoosier HELL
Last seen: 1 year, 7 months
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Re: agar ???? [Re: vatoloco]
#628791 - 05/13/02 08:45 AM (15 years, 11 months ago) |
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Edited by ChromeCrow (08/24/02 06:31 PM)
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johnwsmoke
member
Registered: 03/17/02
Posts: 104
Last seen: 7 years, 2 months
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Way to go CC!!! That's the type of experimentation I like to see!
I think that when you transfer you will see the quality growth you seek!!!!
Right ON!
JWS
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Jon was a crippled, midget, lezbian boy- but he stood 10' tall with a KNIFE...
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