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Offlineble0t
newbie
Registered: 09/12/01
Posts: 33
Last seen: 21 years, 10 months
Jars not doing anything?
    #394382 - 09/14/01 02:46 PM (22 years, 11 months ago)

On Monday of this week i put 20 jars together. Now four days later im still not seeing anything inside the jars. Should I be worried about this? I followed the MMGG prefectly except i might not have let them cool enough. If that is the case can I use my other syringes and put them in the jars? Or is it too late? As you can probably tell im new to this so any help is apprecaited. Thanks!

ble0t



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Am I right? Well, now I'm lonely and I'm right....I'm lonely and I'm right.

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Invisiblecarbonhoots
old hand

Registered: 09/11/01
Posts: 1,351
Loc: BC Canada
Re: Jars not doing anything? [Re: ble0t]
    #394463 - 09/14/01 04:05 PM (22 years, 11 months ago)

Just give it few more days. Spores germinate best at 88F, after this, 80-84 is good for mycelial growth, so you could raise your incubation temp for a day to help germination.

Yes, you could use your other spore syringes as long as it's the same species (psylicybe cubensis I'm assuming) but it's unlikely your jars were too hot.



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  -I'd rather have a frontal lobotomy than a bottle in front of me

CANADIAN CENTER FOR POLICY ALTERNATIVES

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Offlinetchyted
miestro
Registered: 09/03/01
Posts: 526
Loc: WA near seattle
Last seen: 9 years, 11 months
Re: Jars not doing anything? [Re: ble0t]
    #394791 - 09/14/01 11:49 PM (22 years, 11 months ago)

spores are killed at a little over 100 F. were your jars that hot? patience is a virtue. after ten days with no sign of growth, open and smell one jar. no nasty smell? then re-sterilize the jars and re-inocculate, including the one you smelled. bad smell. . . start over.


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Offlineble0t
newbie
Registered: 09/12/01
Posts: 33
Last seen: 21 years, 10 months
Re: Jars not doing anything? [Re: ble0t]
    #394996 - 09/15/01 09:12 AM (22 years, 11 months ago)

Okay here are the new things going on...the temp is about 79-80 degrees right now where the jars are. I am now seeing a slight amount of whispy little white growth. Nothing really solid yet. Im guessing only time will tell whether or not this is mycelium or not right?



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Am I right? Well, now I'm lonely and I'm right....I'm lonely and I'm right.

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Offlineble0t
newbie
Registered: 09/12/01
Posts: 33
Last seen: 21 years, 10 months
Re: Jars not doing anything? [Re: ble0t]
    #394997 - 09/15/01 09:13 AM (22 years, 11 months ago)

oh and no i dont think the jars were quite that hot. At least i hope not.



--------------------
Am I right? Well, now I'm lonely and I'm right....I'm lonely and I'm right.

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InvisibleMy Third Eye
old hand

Folding@home Statistics
Registered: 11/02/00
Posts: 641
Re: Jars not doing anything? [Re: ble0t]
    #395006 - 09/15/01 09:53 AM (22 years, 11 months ago)

everytime ive innoculated jars it takes at least 5 days to see the first signs of growth..

The light that fueled our fire then has burned a hole between us so
We cannot see to reach an end crippling our communication


--------------------
suddenly a flaming stealth banana split the sky
like one would hope but never really expect
to see in a place like this.
Cutting right angle donuts on a dime
and stopping right at my Birkenstocks,
and me yelping...Holy fucking shit!

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InvisibleMy Third Eye
old hand

Folding@home Statistics
Registered: 11/02/00
Posts: 641
Re: Jars not doing anything? [Re: My Third Eye]
    #395010 - 09/15/01 09:57 AM (22 years, 11 months ago)

i wouldnt suggest resterilizing especially if you smell something bad..A resterilizing will make your water content drop drastically B if youve got contams its time to start over..some endospores are really hard to kill..plus that theyve already started to destroy the nutrients in your substrate..

The light that fueled our fire then has burned a hole between us so
We cannot see to reach an end crippling our communication


--------------------
suddenly a flaming stealth banana split the sky
like one would hope but never really expect
to see in a place like this.
Cutting right angle donuts on a dime
and stopping right at my Birkenstocks,
and me yelping...Holy fucking shit!

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OfflineTreeofLife
member
Registered: 02/19/01
Posts: 90
Loc: OH
Last seen: 22 years, 8 months
Re: Jars not doing anything? [Re: My Third Eye]
    #395060 - 09/15/01 11:23 AM (22 years, 11 months ago)

you should be fine, as long as the jars smell fine. Sometimes it can take 7 or 8 days to see anything at all

The only thing you have to fear is, fear itself...


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The only thing you have to fear is, fear itself...

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Offlineble0t
newbie
Registered: 09/12/01
Posts: 33
Last seen: 21 years, 10 months
Re: Jars not doing anything? [Re: ble0t]
    #395360 - 09/15/01 06:28 PM (22 years, 11 months ago)

Well I checked out the smell and i would say nothing smells out of the ordinary. I dont know quite what im smelling for but it doesnt reek liek anything so im guessing im alright. Thanks for the help guys.



--------------------
Am I right? Well, now I'm lonely and I'm right....I'm lonely and I'm right.

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