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InvisibleSwami
Eggshell Walker

Registered: 01/19/00
Posts: 15,413
Loc: In the hen house
Innoculated resterilized contaminated jar...
    #564272 - 02/26/02 11:51 PM (15 years, 7 months ago)

After reading about how one of the shroomerites got sick upon opening and breathing near a contaminated jar, I decided to resterilize my bacteria-infested substrate before throwing it out.

As I autoclaved some other fresh jars at the same time, I decided to re-innoculate it it instead of tossing it. If the bacteria were killed would the new spores take over or would bacteria by-products prevent fresh mycelial growth?

I will give the answer in a week or so, but what do you guys think?


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InvisibleOlgualion
Shaman-In-Training
 User Gallery

Registered: 11/14/01
Posts: 1,253
Loc: Currently Earth...
Trusted Cultivator
Re: Innoculated resterilized contaminated jar... [Re: Swami]
    #564284 - 02/27/02 12:00 AM (15 years, 7 months ago)

I have thought about this, but have never tried it because my gut tell's me that it wouldn't work. I really think the major factor will be just how far along the contaminant got. If it was only allowed to go a day or two before resterilization, I think there may be a good chance the mycelium will be okay. Even if it does work, my guess is that yields will be diminished.


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Study the past...
See the future...


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OfflineWorkmanV
Psilocybe Microscopist
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Registered: 03/01/01
Posts: 3,509
Loc: Washington, USA
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Trusted Cultivator
Re: Innoculated resterilized contaminated jar... [Re: Swami]
    #564366 - 02/27/02 01:17 AM (15 years, 7 months ago)

I've done it and it works fine. However, I doubt you will find a growing text that recommends it. I think the bigger danger is oversterilization of the substrate which alters the chemical makeup and inhibits mycelial growth. Two normal 15 psi sterilization cycles doesn't seem to cause that problem.


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OfflineShaw
hick

Registered: 06/28/01
Posts: 2,218
Loc: midwest, usa
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Re: Innoculated resterilized contaminated jar... [Re: Swami]
    #564368 - 02/27/02 01:18 AM (15 years, 7 months ago)

some bacteria create toxic waste products. i doubt these wastes would be destroyed. I would be cautious they would get into the shrooms.


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OfflineSeussA
Error: divide byzero

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Registered: 04/27/01
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Re: Innoculated resterilized contaminated jar... [Re: Swami]
    #564616 - 02/27/02 10:09 AM (15 years, 7 months ago)

My friend did an experiment once to test this idea (more or less). She was having a hard time getting rid of contamination and was trying to figure out where it was coming from. She created four jars, pf-tek style, but no innoculation. She sterilized the jars for 1 hr at 28 psi (in a pelton and crane autoclave). She injected 'dirty' water (no spores) into the jars and placed them into the incubator at 83F and waited for the jars to contaminate. After contamination all four jars were autoclaved for 1 hour at 18 psi (not 28 this time) and the put back in the incubator (83F) untouched. After a week three of the four jars were contaminated again. The fourth jar was still clean after two weeks. My friend now tosses all contaminated jars in the trash rather than trying to reclaim them.


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Offlinehomebrew
member

Registered: 06/02/01
Posts: 117
Last seen: 14 years, 5 months
Re: Innoculated resterilized contaminated jar... [Re: Swami]
    #564627 - 02/27/02 10:21 AM (15 years, 7 months ago)

I don't see why it wouldn't work in theory. However, substrate is pretty cheap (rye is about $1.50/lb here) so I wouldn't bother with the hassle and worrying that would come with resterilization.


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Offlinethem_26
enthusiast
Registered: 05/02/01
Posts: 204
Last seen: 14 years, 11 months
Re: Innoculated resterilized contaminated jar... [Re: Swami]
    #564639 - 02/27/02 10:34 AM (15 years, 7 months ago)

my cat tried this with his jars when they got a yeast infection(apple cider/wine smell). His python recommended vagisil but I told my cat that only works with women. So he resterilized at 15psi for 20-30 minutes and had a good flush. Though hes black he believes only contaims of the same color are scary and says won't play with them. Actually he won't play with any color contaims and wouldn't have tried with the yeast if he hadn't ran out of vermiculite & $.
gotta go... he's about to feed me.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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