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slow cubie colonization
    #386454 -

ok i just spent like 30 min explaining all this and lost it all so this will be much shorter with less detail. basically i have some jars made up using MMGG 3.2 guide to almost all specs. the only thing i changed is: 1 quart jars as opposed to 1/2 quart, boiling jars in water for 1 hour and not pressure cooked, and in one of the jars i added about 25% straw to. all are colonizing, starw one the fastest but still after about 2 weeks now the best are only about 1/4 colonized. these btw are p. cubensis, the jars are halfway submersed in water in an aquarium with a submersable heater keeping water temp at 82-86 (i fluctuate every now and then, maybe 1 day at 83 then teh next at 86. does that help or no difference?) the only thing that i could contribute to slowed growth is taht theyre not in a totally dark environment, its dim in there but def not dark 24/7 (theyre right on the floor in his room). would that slow the growth? at the same time i started these 5 jars we took teh leftover brf/verm and dumped it in his backyard and shot my remaining 2cc of spores on it and that is now fully colonized and i cased it yesterday with peat/verm 50/50, and hope to see pins by this weekend. so i know theyre good spores and a decent substrate, and i think the moisture is ok, if anything a little moist (sparingly scattered small droplets of water on sides of jars). this is my first real attenmpt at growing these babies, but ive read all i can find as far as teks and methods go, also a lot of the cult. posts and probably even more of the advanced posts also. but at this rate they wont colonize the entire quart jars for another 2 weeks or so (almost 30 days total) i hear of mycellum coverig 1/2 pint jars in as little as 10 days, would these quart jars actually take a month to get there? also i have 5 other jars that i prepared maybe 12 days before i started these and they were stoerd in a basement (too cold; about 65 fahrenhiet, thats why i switched to the aquarium setup when we made new jars) and thothey barely started mycellum in the 12 days now that theyve been in the warm water setup they have grown to about 50% colonization but a lot of it is turning from its white to a (what looks like) slightly shriveled yellowish color. not a good solid white but a weak looking bumpy surface. it looks liek mycellum, if i can get mikes digi camera ill post some pix but in the original small places that colonized in the colder climate have turned almost a solid yellow, but its still taking over the cake and it smells like shrooms in there, not mold so im gonna wait that out. any advice on how i can speed this up or maybe a better (and also cost efficient) way of keeping them at temp? im not sure if the still water is collecting bacteria and evaporating it up into the tinfoil and maybe contaminating them?.?.? the 5 new jars seem to be amazingly healthy white mycellum, just growing very slow. any suggestions at all? if you feel i left out any key points please ask away. im no expert but like i have said ive read/researched mushroom teks from here, mushmush.nl, magic-mushrooms.net, and varoius other sites. i havent checked teh pH in teh jars but i didnt think that's be a prob. thanks in advance!


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Re: slow cubie colonization [Re: BeppoMarx]
    #386456 -

Post Deleted a la Obscurity

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Re: slow cubie colonization [Re: ]
    #386571 -

or not enough air exchange.... try loosening the lids, or remove the tin foil, it took over a month for my 1 pint jars to colonize, they take way to long so i switched to 1/2 pint...

You approach the turnstiles and know that when you get there you have to give the man two
dollars or he won't let you inside but when you get there everything goes wrong.

Edited by windex on 09/05/01 10:08 PM.


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Re: slow cubie colonization [Re: windex]
    #386961 -

yeah i figured it might need some more O2. what about contamination? if i loosen the lids and/or remove tinfoil wont the jars be prone to infection? i think my safest bet is to replace the old water in the heating tank and add a bottle of H2O2, and cover the tank with saran wrap. im just leary about infection because its not exactly sterile there. also, another note; would placing them in the dark help at all? thanks again, i appreciate the responses


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Re: slow cubie colonization [Re: BeppoMarx]
    #386965 -

and btw chemical monkey, if youre too lazy to read my post and help people (me) out; dont assume that you have the answer because i addressed those issues directly in my text. youre just wasting your own time posting it.


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Re: slow cubie colonization [Re: BeppoMarx]
    #386993 -

You know Beppo, it is very hard to read your post with no capitals or paragraphs etc. It reads like a stream of consciousness beatnik poem!

10 days is good going for 1/2 pints and Quart jars are going to take a lot longer than 1/2 pints - they are 4 times the size for starters. I also wouldn't have thought that an hour's boiling would be long enough for that sort of volume but let's hope it is.





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Re: slow cubie colonization [Re: BeppoMarx]
    #387128 -

Post Deleted a la Obscurity

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Re: slow cubie colonization [Re: ]
    #387217 -

i didnt say take the lids off, i said loosen the band, besides you should have the verm layer to prevent such things they arent as prone to desiese as many think..

example: I have a halfpint jar, my mom maid white rice for dinenr about 7 days ago, so i filled it up, put it in the microwave for two minutes. put the top on, not tight, not loose. The next day I squirted what i had left in a two month old syringe thats been sitting in one of my drawers. three days ago i TOUCHED the rice with my un-washed finger (after a full day of work) to see how dry the rice was. Today still no contaims and the whole deal is starting to colonize nicly, covering the spot i touched.

You approach the turnstiles and know that when you get there you have to give the man two
dollars or he won't let you inside but when you get there everything goes wrong.

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Re: slow cubie colonization [Re: windex]
    #387256 -

didnt you see the official moron in parenthesis? give him a break..lol

The light that fueled our fire then has burned a hole between us so
We cannot see to reach an end crippling our communication


--------------------
suddenly a flaming stealth banana split the sky
like one would hope but never really expect
to see in a place like this.
Cutting right angle donuts on a dime
and stopping right at my Birkenstocks,
and me yelping...Holy fucking shit!

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Re: slow cubie colonization [Re: My Third Eye]
    #387354 -

Post Deleted a la Obscurity

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Re: slow cubie colonization [Re: BeppoMarx]
    #387608 -

it is next to impossible for a begginer to get a colonized quart jar of anything whithout pressure cooker sterilization. next to impossible.

refer to the PF technique, where he discourages even a pint jar because colonization time will be too long and long incubation periods invite contamination.

no bad smell with slow colonization and all that you have written sounds like yeast competition to me.

your absolute best bet would be to take at least one jar outside and treat it exactly like the material that you have doing well, except be sure to keep them separate. if there is a contam that we have missed, there is still a good chance that your mycelium could survive outdoors.

my early failures, and i had lots of them, were a result of biting off more than i could chew


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Re: slow cubie colonization [Re: ]
    #387808 -

sry monkey, didnt mean to be rude. i was just pissed yesterday (mostly cos i just lost 6 of my 10 jars) but when i emptied them out yesterday i found the problem and [with apology to chemical monkey, again] they were too dry. i thought it had sufficient moisture because they always droplets formed on the underside of the lid when i opened them up, but upon removal of the substrate it was very dry, which is probably why the mycellum slowly faded away into a grey color, it dehydrated and died. i could notice also some shriveling of the mycellum, that should have tipped me off but oh well, im a newbie. ill add some water to the remaining jars today and see if they take off a lil faster. by the way, the ones outside that i cased have great healthy mycellum peaking thru the peat and im hoping for pins today and maybe some good edible fungus by monday or so. thanks for all of your help on this, its really appreciated.


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Re: slow cubie colonization [Re: BeppoMarx]
    #388205 -

Post Deleted a la Obscurity

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Re: slow cubie colonization [Re: tchyted]
    #388573 -

tchyted- yeah i crumbled the 5 jars that were dehydrated outside on top of about 1/2" verm, sprinkled some rye flour on them, watered like crazy and threw a thin layer of verm on top to try and keep it moist. the 1 other jar that i lost had good healthy mycellum in all 4 spots but (damn) lipstick mold started up near the bottom and on 1 side. so i manually dug out the 4 mycellum parts, crumbled 1 in with teh 5 other ones in teh bed and the other 3 got mixed with rye flour and a lil more verm and prepared just like the other ones. hopefully they yield a shroom or 2 in the near future, im moving to AZ in a few weeks and i want to get at least something out of this experience; i sure as hell cant cult. outside in the fucking desert! im gonna plant a bunch of ps. cyanescens patches up here in NY in various places and mbe next summer when i come back there will be some, and hopefully some of my cubie patches i have scattered around will be lively! all in due time, i hope... anyone know if good mycellum is established will cyanescens or cubies fruit after being under snow for 7 mos?


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Re: slow cubie colonization [Re: BeppoMarx]
    #389151 -

Ps. Cyanescens love the cold!! But they are the devil's babies ;-)



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