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OfflineAbeZard
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Registered: 07/20/01
Posts: 164
Loc: Charleston, SC
Last seen: 15 years, 24 days
3 generations of green has me frustrated
    #385698 - 09/04/01 08:11 PM (15 years, 3 months ago)

I know this could be posted in contams, but posts don't seem to get as much attention there (moderators, if you are listening, I put in a vote to combine the 2).

I am very frustrated at having been through 3 generations of jars and not yet having grown even one shroom! The reason? Green, to put it simply. Green has shown up on casing and cakes, in a hidden sea-chest with an ultrasonic terrarium and in a cooler+perlite terrarium . . .
I feel I have done all the steps right. . . all I can figure is green has gotten in my room and doesn't want to leave. Almost all the green has shown up first in the sea chest terrarium (which I sprayed with chlorine+water before the last batch) but, also, some has been in jars before they have been birthed.

Currently, I have 13 jars within a week or two of birth time and I want at least some of those babies to produce (I am not giving up yet!).

I wonder if I need to leave them invitro to be sure, or spread my risk. At the least, I feel I need to dissassemble the sea-chest (no small task) and clean everything thoroughly and/or give up on it and get a new styrofoam cooler to use.

ANy ideas?????

Thanks for any help you give to a frustrated newcomer to this fascinating hobby.

Abe Zaardvark
From historic coastal town in Southeast


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Abe Zaardvark
From historic coastal town in Southeast


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InvisibleZen Peddler
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Re: 3 generations of green has me frustrated [Re: AbeZard]
    #385715 - 09/04/01 08:38 PM (15 years, 3 months ago)

to give yourself a string chance of beating the green, start again. Buy new syringes - even spore syringes. Overkill bigtime with all sterilization - PC for an hour and a half, flame the needle AND wipe it with an alcohol swab, where gloves wiped with alcohol when innoculating, where a mask so you dont breath on the needle head (although ive been told that you cant get green that way).
Also put alcohol swabs over the inno. holes in your jars so that no nasties can float on in (generally, as the substrate colonises and shrinks, your ramsey seal can start falling down the sides of the substrate and become ineffective)
Just be a paranoid nutcase when it comes to being sterile, and you will have much better success!!



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InvisibleBillyblastoff
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Re: 3 generations of green has me frustrated [Re: AbeZard]
    #385719 - 09/04/01 08:41 PM (15 years, 3 months ago)

Yep, that green "trichaderma" is it? is a job hazard as far as this hobby goes. Given enough time it will almost show up anywhere "jars, cakes, casings" I think it has alot to do with excessive moisture combines with enough time along with issues of sterility. Dont give up, watch your moisture, clean out that chest and maybe invest in some filter disks to speed up colonization of your jars. You'll get it down...


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"Imagination is more important than knowledge. For while knowledge defines all we currently know and understand, imagination points to all we might yet discover and create."

"There is a wealth of information built into us, tucked away in the genetic material in every one of our cells. The psychedelic drugs allow exploration of this interior world, and insights into its nature."

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InvisibleZen Peddler
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Re: 3 generations of green has me frustrated [Re: Billyblastoff]
    #385723 - 09/04/01 08:48 PM (15 years, 3 months ago)

yeah - ive lost sooo many jars to the green - i lowered the temp of one of my jars from around 25c to about 16-14c and this completely stalled the green shit - still lost the fucking cake though!



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OfflineAbeZard
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Registered: 07/20/01
Posts: 164
Loc: Charleston, SC
Last seen: 15 years, 24 days
Re: 3 generations of green has me frustrated [Re: Billyblastoff]
    #385725 - 09/04/01 08:51 PM (15 years, 3 months ago)

Thanks for the encouragement (and, that picture), Billy.
Colonization speed doesn't seem to be a problem- these half pint jars colonized within 2-3 weeks.
I am not ready to tackle cleaning out that sea chest yet- I'll need a good weekend for that . . .
Is invitro a safer way to go?

Abe Zaardvark
From historic coastal town in Southeast


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Abe Zaardvark
From historic coastal town in Southeast


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InvisibleBillyblastoff
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Re: 3 generations of green has me frustrated [Re: AbeZard]
    #385733 - 09/04/01 09:03 PM (15 years, 3 months ago)

Yeah that pic is a classic, I dont know if I'll ever tire of it...lol
Anyway if you want to almost asure yourself of some shrooms "open up the jar and leave the dry verm in" but dont expect really good yields. I got one going right now that started pinning in the closet even before it was 100% colonized so I brought it out put it in the perlite chamber and I got 2 4" shrooms and a few aborts outta it in like a week. I have no doubt i'll get some more though. Try different teks it only adds to your knowledge I say...



Tell me something good then I might agree with you..


--------------------
"Imagination is more important than knowledge. For while knowledge defines all we currently know and understand, imagination points to all we might yet discover and create."

"There is a wealth of information built into us, tucked away in the genetic material in every one of our cells. The psychedelic drugs allow exploration of this interior world, and insights into its nature."

Tell me something good then I might agree with you....the Smithereens


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OfflineAbeZard
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Registered: 07/20/01
Posts: 164
Loc: Charleston, SC
Last seen: 15 years, 24 days
Re: 3 generations of green has me frustrated [Re: Billyblastoff]
    #385737 - 09/04/01 09:05 PM (15 years, 3 months ago)

Bluemeanie-
I have new spore syringes already- 3 types from 2 sources. THe last two contam'ed jars were from 1 of those. The 13 remaining, unbirthed jars are in the incubator and still showing no signs of contams but I am worried to birth them in the hostile environment so much green has spread in.
My first two batches came from a wild spore print, gone now.
THanks

Abe Zaardvark
From historic coastal town in Southeast


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Abe Zaardvark
From historic coastal town in Southeast


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InvisibleBillyblastoff
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Re: 3 generations of green has me frustrated [Re: Billyblastoff]
    #385752 - 09/04/01 09:16 PM (15 years, 3 months ago)

Oh BTW Ive grown out shrooms from a casing with the trich in it. Its not exactly a death penalty when it shows up when you already have pins and shrooms. I try and scoop it out and replace with fresh casing but unless you take out alotta casing around the contamed area it will most likely come back in my short experience.
Thats enough advice for me tonight, Im outta here...




Tell me something good then I might agree with you..

Edited by Billyblastoff on 09/04/01 08:19 PM.



--------------------
"Imagination is more important than knowledge. For while knowledge defines all we currently know and understand, imagination points to all we might yet discover and create."

"There is a wealth of information built into us, tucked away in the genetic material in every one of our cells. The psychedelic drugs allow exploration of this interior world, and insights into its nature."

Tell me something good then I might agree with you....the Smithereens


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OfflineNumba9
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Re: 3 generations of green has me frustrated [Re: Billyblastoff]
    #385790 - 09/04/01 09:52 PM (15 years, 3 months ago)

I changed over to the oven tek..Oven on lowest setting and do all my work with that rising warm air. I`ve had great success since i changed over from my old cardboard box lol. and a drop light.. give the oven tek a try.. of course still use an alcohol flame and either wipe syringe after flaming with alcohol or lysol each time before entry into jar. Hope you have better luck Bro





?But I don?t want to go among mad people,? Alice remarked.
?Oh, you can?t help that,? said the Cat: I`m mad,Your mad.we`re ALL MAD!!!! hahahahaha"



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Remember..what the Door Mouse said..."Feed your Head".......... Jefferson Airplane


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Offlinetchyted
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Registered: 09/03/01
Posts: 526
Loc: WA near seattle
Last seen: 2 years, 2 months
Re: 3 generations of green has me frustrated [Re: AbeZard]
    #385939 - 09/05/01 12:20 AM (15 years, 3 months ago)

expose your incubating jar to light. leave them in the jars until you see actual big pin or small shrooms.

at that point, the cake should be fairly green resisitant.



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OfflineBBShrizz
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Registered: 03/01/01
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Re: 3 generations of green has me frustrated [Re: AbeZard]
    #385983 - 09/05/01 01:44 AM (15 years, 3 months ago)

i hear you, abe.

no matter what i've tried, i've been victimized by the green mold. I have the HEPA going 24/7, douse the place periodically in alcohol, have tried straw, dung, crumbled cakes, etc. about the only thing i can make work is straight cakes.

the frustrating thing is that no matter what i do it still fucks me.



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Invisiblepuscle
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Registered: 01/07/01
Posts: 4,539
Loc: NY
Re: 3 generations of green has me frustrated [Re: AbeZard]
    #386049 - 09/05/01 03:24 AM (15 years, 3 months ago)

Abe, you,re on the right track. I'm glad you went with fresh syringes, that alone could make all the difference. The sea chest makes me nervous though. All that wood and fabric, no real way to clean it.
P. S., This is the toughest time of yhe year. Summer is cruel to all of us that live in a hot and humid environment.



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InvisibleNDK
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Registered: 07/13/01
Posts: 186
Re: 3 generations of green has me frustrated [Re: puscle]
    #386062 - 09/05/01 04:00 AM (15 years, 3 months ago)

My last lot of jars have got mold. It's kind of greeny-bluey-grey and has no noticeable smell. It's well annoying especially when you have waited more than a fortnight in eager anticipation only to have your hopes dashed.

I'm convinced that a fair proportion of spore syringes are just not sterile. When you are getting contamination in nearly every jar and you are pressure cooking for 1 hour+ I'd start to look at your supplier.



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Offlinehomebrew
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Registered: 06/02/01
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Re: 3 generations of green has me frustrated [Re: AbeZard]
    #386166 - 09/05/01 10:10 AM (15 years, 3 months ago)

As far as sterilizing the sea chest. Before you disassemble it, try this which has worked for me in the past on some really funky brewing equipment and nasty moldy coolers. If the chest is fairly air tight I think it will work for you too. Take a generous amount bleach (don't dilute it) and pour it into a container that has a good amount of open surface area; personally I use mixing bowl. Put the bowl in the chest and close it up. The chlorine will do its job over the course of a few days to a week as the vapors get in every nook and cranny of the wood. If the chest has a lot of cloth like someone said, I wouldn't go with this route for the obvious reasons of what bleach does to fabric.



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