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Registered: 04/19/01
Posts: 623
Loc: Canada
Last seen: 21 years, 10 months
safe to loosen & flip jars?
    #373333 - 08/16/01 09:15 PM (22 years, 1 month ago)

My friend has some jars that she's trying to grow invitro and so far, nothing. They've taken a long time to completely colonize (about 5 weeks) and now they are just sitting there.

She wants to know if she could...
a) loosen the lids about a 1/4 turn to allow some air exchange
b) put the jars outside for 1 night; a cold shock.
c) flip the jars completely upside down. She already has them on their sides; is there any difference?

They are 100% colonized, so the risk of contams is not too great, correct? Oh, and she has tinfoil on the tops of them. Should she remove these to allow air exchange?

Thanks again...



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Registered: 08/17/01
Posts: 4
Last seen: 20 years, 8 months
Re: safe to loosen & flip jars? [Re: Shiznitz]
    #373486 - 08/17/01 02:48 AM (22 years, 1 month ago)

I would say yes. They definately need air and they are unlikely to get any contamination at this stage. I'd loosen the lids or take the tape of the hole and turn them upside down. Dy now they should have shrunk a little so they will move in the jar and pull air in which will speed things up. If you are wanting to wait until they start to pin in the jar ilke PF suggests then some light is needed too. I'd guess the reason they took so long to colonise is the lack of air exchange so far.

I wouldn't bother about cold shocking them it doesn't seem to make that much difference but others may disagree. I'd worry about putting them outside even when fully colonised.

Your other option is to birth em into your terrarium.



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genius of love
Registered: 01/06/01
Posts: 4,539
Loc: NY
Re: safe to loosen & flip jars? [Re: Shiznitz]
    #373522 - 08/17/01 04:47 AM (22 years, 1 month ago)

Yes, yes and yes. Tinfoil off and plenty of light.

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