Sterilization and point of implementation. - Mushroom Cultivation

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Freefly
mycoman

Registered: 05/30/01
Posts: 40

Sterilization and point of implementation.

#364809 - 08/01/01 02:05 PM (23 years, 10 months ago)

I just got done with my first ever flush and now it's time to make new jars. After thinking about my sterilization procedures of preparing jars I think I may change my tech to save some time. The first time I created a complete sterile enviroment to prepare the jars, pressure cook, and inoculate. This took me half a day just to create the enviroment, then a second half slaving away trying to get all the procedures done at once in a hot exclosed kitchen.
My question is this. Why is a complete sterile enviroment nessacary for the preping of the jars when the pressure cooking is going to kill everything anyway? Wouldnt it suffice to have a sterile enviroment just from the point of taking the foil off the jars and innoculation?
I would like to get the opinion of some of the senior members here. As well as everybody elses opinion.

Thanks

Freeflying...The fastest non-mechanized sport in the world.

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puscle
genius of love

Registered: 01/06/01
Posts: 4,539
Loc: NY

Re: Sterilization and point of implementation.

[Re: Freefly]
#364942 - 08/01/01 05:48 PM (23 years, 10 months ago)

It's not needed for pressure cooking. Yes, keep it clean when you inoculate. You're working to hard.

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fenster21
Stranger

Registered: 07/21/01
Posts: 5
Last seen: 23 years, 10 months

Re: Sterilization and point of implementation.

[Re: puscle]
#365030 - 08/01/01 08:21 PM (23 years, 10 months ago)

you don't need a sterile environment to prepare your substrate before you PC it

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jonnyshaggs420
Pooh-Bah

Registered: 08/08/00
Posts: 1,965
Loc: Mid-West
Last seen: 19 years, 8 months

Re: Sterilization and point of implementation.

[Re: Freefly]
#365032 - 08/01/01 08:32 PM (23 years, 10 months ago)

Sterility is usually over stressed in cultivation techs because alot of newbies won't take proper techniques when you need them. For preparing the jars sterility doesn't matter cuz your PCing everything your using. If you are innoculating through holes in the lid of the jar, ala PF/MMGG, then just flame the needle between jars, thats all the sterility needed. But if you are taking the lid off and innoculating then a sterile environment is definately needed.

I don't care about jack shit, I just wanna pick my nose!!!
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birdbrain
Stranger

Registered: 05/21/01
Posts: 10
Last seen: 23 years, 10 months

Re: Sterilization and point of implementation.

[Re: Freefly]
#365056 - 08/01/01 09:09 PM (23 years, 10 months ago)

use a clean box it's easy to build and is worth it's time!!!

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Freefly
mycoman

Registered: 05/30/01
Posts: 40

Re: Sterilization and point of implementation.

[Re: Freefly]
#365157 - 08/01/01 11:43 PM (23 years, 10 months ago)

I have been going to extremes to get a sterile environment and putting alot of effort into it. After I did this for my first time I realized that prepping the jars didnt need as much sterility as I originaly thought. Although I see how other procedures like taking prints may warrant my efforts at sterility.

Thanks for the responses y'all.

Freefllyskydiving

Freeflying...The fastest non-mechanized sport in the world.

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