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pyromaniac
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Glad containers
#339912 - 06/13/01 01:58 PM (23 years, 7 months ago) |
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Ok, 3 days ago, to the hour, I tried innoculating some glad containers (you know, the ones that are suppose to be cheap enough to throw away, w/e). I am trying it because about a week ago I saw a thread about something similar to this. Anyway, I did several of these containers with brf/verm and microwaved them for 8 mins each in about 1/2 inch of water. I just about burnt myself each time I took them out of the microwave so I am sure they are sterile. Anyway, I was wondering if anyone has had sucess with this and if there is a greater conamination rate. Im sure there is, being that they are microwaved, but it seemed like something to try due to how easy it is. Plus, It only costed a couple bucks to make them up and innoculate so what have I got to lose.
Anyway, I will see how sucessful I am at this and maybe consider trying it more if it works out.
---Pyromaniac---
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puscle
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Re: Glad containers [Re: pyromaniac]
#339958 - 06/13/01 04:14 PM (23 years, 7 months ago) |
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They work well if you steam them as PF style jars.
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them_26
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Re: Glad containers [Re: puscle]
#339988 - 06/13/01 04:55 PM (23 years, 7 months ago) |
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How much do the gladware containers run for?
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pyromaniac
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Re: Glad containers [Re: them_26]
#340023 - 06/13/01 05:47 PM (23 years, 7 months ago) |
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Well, I got two different sizes from two different brands. One of them I believe holds 4 cups, the other is like 1 1/2. The brands were ziplock and glad and they ran about 3 bucks for 6 of the small ones or 3 bucks for 4 of the large ones. I got mine a Fred Meyer's, though I'm sure most stores carry them, theyre in the same area as the paper plates and paper bags used for sack lunches.
---Pyromaniac---
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pyromaniac
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Re: Glad containers [Re: pyromaniac]
#340026 - 06/13/01 05:55 PM (23 years, 7 months ago) |
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I peeked in at them a little while ago and saw what looks like just the beginning of some growth. I could be wrong though. I could be seeing something different due to the glare of the lid and the color of the substrate. It looks like a little spot of mycelium just a couple mm wide. It seems like it may be a bit too early, due mainly to the fact that the temp is a bit lower then normal but I guess only time will tell.
---Pyromaniac---
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pyromaniac
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Re: Glad containers [Re: pyromaniac]
#340090 - 06/13/01 07:06 PM (23 years, 7 months ago) |
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Nope, definitely mycelial growth. I checked a bout 3 minutes ago and peeled back the tape far enough to see that there was a mass of mycelial growth about 1.5 cm wide right under one of the holes on one of the larger containers of innoculated substrate with cambodian spores. This is in three days at an average incubation temperature of 70-85 degrees. Sucess so far, most of these prints, if sucessful, will be going to ralphster and the FSR due to the fact that I won that print in one of his contests. Thanks Ralph.
---Pyromaniac---
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shroomeritus
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Re: Glad containers [Re: pyromaniac]
#340401 - 06/14/01 03:22 AM (23 years, 7 months ago) |
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hey there pyromaniac,
glad to see that your gladware containers are working out. i was just wondering if you were planning on trying to shake those puppies up a bit. or if it would even be possible to? please keep us informed. that seems like an awesome tek if everything goes well, especially with the shaking. a very awesome tek.
-------------------- -the sweet is never as sweet without the sour
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jonnyshaggs420
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Re: Glad containers [Re: pyromaniac]
#340428 - 06/14/01 04:56 AM (23 years, 7 months ago) |
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The microwave can reach extremely high temps so sterilization is not a problem.
Tired of pimping yourself on the corner to afford spores? Well throw out that red dress and go to the FSR, for spores even you can afford.
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puscle
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Re: Glad containers [Re: pyromaniac]
#340444 - 06/14/01 06:04 AM (23 years, 7 months ago) |
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Log in to view attachment
Like this. (see pic)
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auto59009
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this may be just me, but whenever verm goes in the microwave it comes out with its container melted (if plastic) or cracked (if glass)... is this a humidity thing or heat capacity of the verm?
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Jared
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Content Removed.
Edited by Jared (04/01/04 01:14 AM)
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Freefly
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Re: Glad containers [Re: Jared]
#340676 - 06/14/01 01:30 PM (23 years, 7 months ago) |
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Yesterday i used a plastic mixing bowl to sterilize my vermic. in the microwave and ended up with verm imbeded in the bottom of the bowl. No amount of scrubbing got it out.
Freeflying...The fastest non-mechanized sport in the world.
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pyromaniac
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Re: Glad containers [Re: Jared]
#340684 - 06/14/01 01:42 PM (23 years, 7 months ago) |
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Verm does just fine in the microwave. Also, the plastic dishes shouldn't melt so long as they are sitting in a dish of water which has about 1/2 - 3/4 inch of water in it. You should also set the container on a rag in the microwave.
The small containers that I used look exactly like that attached one.
If I had a camera I would take pics, I think I might get a disposable one because I have a really really nice scanner.
---Pyromaniac---
-------------------- I admit it, Im a fungal-feliac
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them_26
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Re: Glad containers [Re: pyromaniac]
#340700 - 06/14/01 02:02 PM (23 years, 7 months ago) |
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I've read posts about others having a melting problem also. Why not just steam like the man said? If the water/rag doesn't work for ya, that is.
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Jared
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Deleted [Re: Jared]
#340751 - 06/14/01 03:10 PM (23 years, 7 months ago) |
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Content Removed.
Edited by Jared (04/01/04 01:14 AM)
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Shecky
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Re: Glad containers [Re: auto59009]
#340819 - 06/14/01 05:06 PM (23 years, 7 months ago) |
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I don't know about you guys and your goddamn gladware containers. I read the same thread and ran out to wally world to get some. They were 8.5 oz. pretty close to 1/2 pint. I nuked em for 8 min. in an inch or so of water. When i opened the door, the lid waws off the verm. was fucking everywhere, and the cake was harder than a rock. WTF i thinks to myself?
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pyromaniac
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Re: Glad containers [Re: Jared]
#340879 - 06/14/01 06:32 PM (23 years, 7 months ago) |
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Something to look forward to, eh. I wouldnt be in the least surprised if that happens to me too. I wanted to see, however if this would work and so I am trying it, if it doesnt, oh well I'll just use jars next time :). Oh, and Shecky, I had the 4 holes for the syringe needly poked in the lid before I microwaved it. That way steam could escape instead of building up and then blowing the lid off. I think that probably you hadnt put the holes in it and that caused the lid, and some vermiculite to blow off. Then, there would be no lid to stop the water in the cake from drying so all of the water would evaporate and make the cake hard. Then, when I took it out there ws team shooting out of the holes and I taped em off. The steam shooting out should have kept any spores from entering.
If it gets contams, oh well I just won't try again, this is just an experiment.
---Pyromaniac---
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Nagual
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Re: Glad containers [Re: Shecky]
#340881 - 06/14/01 06:36 PM (23 years, 7 months ago) |
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Didjya leave the lid(s) off alittle to give the pressure somwhere to go? Did you not use enough water? Maybe the 8 minutes was too much time? The microwave may have some advantages over regular stovetop sterilization, since they cook from the inside out, but as there are no firmly established microwave teks yet you should be more aware of each and every step you take with it. Good luck microwavers.. stand too close though and you WILL grow an extra head.
o0O)}>there is no real death, life is only a dream and we are the imagination of ourselves<{(O0o
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Nagual
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Re: Glad containers [Re: pyromaniac]
#340889 - 06/14/01 06:47 PM (23 years, 7 months ago) |
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That's what I mean, like seeing the steps you take and understandinding why they work out; I like how the steam pressure keeps everything sterile. Sounds like the beginnings of a valid technique. Godspeed Pyro.. omani... umm.. Please don't burn my house down?!
o0O)}>there is no real death, life is only a dream and we are the imagination of ourselves<{(O0o
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....(_______)
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gray1
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Re: Glad containers [Re: auto59009]
#341255 - 06/15/01 06:53 AM (23 years, 7 months ago) |
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gladware containers are cheaper, but for a longer term investment, rubbermaid makes some nice, low (about 1-2") rectangular 5" x 8" containers that are microwaveable for around $5 each. these are nice because they are also autocalveable, you can sterilize the medium right in the container and be good to go. it works in the autoclave if it has "pp" imprinted near where the recyclable imprint is on the bottom of the container.
gray1
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Shecky
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Re: Glad containers [Re: gray1] 1
#341564 - 06/15/01 03:09 PM (23 years, 7 months ago) |
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Vive Le GladLock!!! or something like that, I guess. Hey Pyromaniac, I did actually have the holes pre-punched in the lid. However, I sealed the lid AND taped the holes before sticking em in there. Really, this Tek could work I guess. I prolly just fucked it up for myself. Maybe I'll give it another shot. BTW Pyromaniac, if you have any luck with yours (since they're already started) let me know. Shecky.
Hey, a lobotomy is better than nothing at all.
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pyromaniac
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Re: Glad containers [Re: Shecky]
#341698 - 06/15/01 05:49 PM (23 years, 7 months ago) |
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I will be sure to let all know how it goes, and just pray that it works. This seems like such a worthwile waste of cakes if it gets contams anyway cuz there isnt any microwave teks that I know of that are good. Oh, and I think that your microwave would be petty sterile anyway and anything that does make it into your container if the lid is off would be quickly killed.
Having the holes pucnhed in the lid really did keep the lid from popping off. I know this because on all of them the lid never even came close to coming off. The last 2 containers I forgot to put the holes in for and the lids started swelling and making funny sounds like they were going to pop off. I opened the micro and put holes in the lids and all of the built up steam came out and the lid went back to normal and they didn't swell again.
So far there is about 3 cm. or about 1 inch of growth. And, all of it is under the holes where I inoculated, and all of it is a fluffy white. Unless it is some kind of contamination that looks exactly like mycelium, then my containers are still holding up and growing great, if not a tad bit slow.
And I promise I wont burn anyones house down. And I hope this includes mine :).
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Disc0rd
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Re: Glad containers [Re: pyromaniac]
#341760 - 06/15/01 07:36 PM (23 years, 7 months ago) |
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Hey, Pyro.
I'm curious to see how this goes. I'm using the jar method at the moment, just did some casings the other day. (Yeah! ) I've been curious about microwave teks for a while, though. The first shroom guide I read was the "Fast Food of the Gods" microwave method, but it seemed somehow unreliable. (I don't remember why offhand--maybe I'll go and reread it so I can comment in more detail.) But I figure it should be possible to come up with a good nuker-tek. Let us know how the experiment does as it progresses! I'd like to try a method like this someday. Best of luck to ya!
p.s.:
I went back and skimmed the FFG method I mentioned. It calls for ingredients such as glycine, dextrose, trace minerals, and oystershell powder [isn't this perlite?] to be added to the substrate. I can't help but wonder if those rather arcane ingredients--compared to the usual verm&rice cake--are really necessary, and what exactly their purpose is. I found that some of them, like glycine, seemed hard to obtain. (i.e. $$) Also the FFG tek implied a particularly high yield, and I can't figure out what detail(s) in the tek would support that.
the link to the tek should be http://www.erowid.org/plants/mushrooms/mushrooms_cultivation8.shtml, or you could search for it.
What do you think of that tek, Pyro (if you feel like reading it, or perhaps you already have) compared to your own experiment?
I'll be checking on this thread. Hope you get a nice batch of shroomies out of this--you're right though--even if you lose the material to contams, you'll have learned a lot about possible future tweaks to the method.
Rock on.
-- Disc0rd
"In the 60's people took acid to make the world weird. Now the world is weird, people take prozac to make it normal."
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Disc0rd
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Re: Glad containers [Re: Disc0rd]
#341767 - 06/15/01 07:55 PM (23 years, 7 months ago) |
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Incidentally, the author of the FFG method says that he prefers "mycelium water" to spore water. What is this "mycelium water"? I mean, I have a guess as to the basic idea, but how would one make it? Has anyone else compared mycelium and spore injection?
-- Disc0rd
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pyromaniac
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Re: Glad containers [Re: Disc0rd]
#341973 - 06/16/01 01:36 AM (23 years, 7 months ago) |
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I have not yet made mycelium water, though I want to. I just need light malt extract which is relatively easy to get ahold of online.
I have read that microwave tek, and just reread it and have decided that I don't know what half of those ingredients are. That was the only other microwave tek that I knew of and since it didn't seem all that easy or reliable, I wanted to try my own.
A post I saw a week or so back was the inspiration. That is what basically gave me an idea of where to start. Since I inoculated my jars I have already come up with a couple more ideas of how to improve upon this idea. Right now, my cakes are not getting much air because I had to tape over the holes instead of using verm and foil. Foil would have really screwed up my microwave so I left that part off. But I figure that the indent in the lid can be filled with some verm after you take it from the micro and then place foil over it. I have also been thinking about trying it with one of the sandwich containers. Those things are wider, but thinner. If you put a couple more holes in the top of one of those containers and spent just a bit more syringe on it, you could have it 100% colonized in like half the time. Anyway, these are just ideas. If anyone has anymore please post them and I will try them out on my next experiment. I am going to keep trying this until I find a way that works.
With time and experimentation we may be able to pull something out of this.something out of this.
I am just tired of not being able to be 100% sure about the sterility of my jars due to not having a P.C.
Oh, and the address to the mycelium water tek is http://shroomery.org/findorgrowthem.php?View=docs&doc=48
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Disc0rd
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Re: Glad containers [Re: pyromaniac]
#342008 - 06/16/01 04:22 AM (23 years, 7 months ago) |
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Thanks for the link. :) (Sorry, I should have searched--I wasn't sure how obscure mycelium water was.)
You've got some good ideas there for improving the method. (I wish I were a bit less of a newbie--otherwise maybe I could try and help contribute. In the meantime I'll just be watching the thread, heh.) Only thing I can think of is "birthing"--it seems that the FFG ignores the terrarium idea and fruits in the buckets, which implies that there's some room in there above the substrate.(?) How much space have you got between the substrate and the lid?
...Hey, maybe someday soon there'll be a Pyro Tek. ;) Catchy, eh?
-- Disc0rd
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Shdwstr
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Re: Glad containers [Re: pyromaniac]
#342124 - 06/16/01 11:00 AM (23 years, 7 months ago) |
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Pyro - When using a microwave for sterilizing, your right, using foil would wreak havok in a microwave. But then again, you might enjoy all the sparks and flames that could result. The need for the tinfoil when boiling or pressure cooking your substates, is to prevent the steam from condensing and entering your jar, adding to much moisture. Using a microwave gives the opposite effect. The microwave heats the substrates moisture, creating the sterilizing steam. The comtainer, either jar (Plastic lid only) or Glad type must be vented to allow the pressurized steam to escape. Without venting, the Containers top will pop off or the jar would explode. The exhaust fan in the microwave removes the excess steam inside the microwave. Rather than excess moisture entering the jar/container, the problem now is loosing too much moisture from the substrate. This can be remedied by making your substrate SLIGHTLY more moist at the start, and being careful to not over nuke it.
Keep in mind that nuking times are dependent on the amount your are cooking. I have been using 15 min. for 2, 1qt. jars of steeped and drained birdseed, fitted with plastic lids and filter discs. So far I have not had any contam problems. If you are doing cakes in smaller jars you will have to adjust your cooking times (10 minute min.) accordingly.
You can still use a top vermiculite barrier on your cakes. If you are not using filter discs, cover the inocculating holes in the plastic lid with micropore tape. This will allow steam pressure to escape when nuking a still provide some protection against contaminates afterwards.
Hope this helps,,, or at least gives you more ideas to play with.
Enjoy
Shdwstr
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PunGiVorous
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Re: Glad containers [Re: Shdwstr]
#342216 - 06/16/01 01:46 PM (23 years, 7 months ago) |
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if any 1 is intrested i will trade ex-light malt or dextrose for any useful myco supplys you might have an abundance of like spores, BTW malt will usaly run ya like 10-15 bux for a big ass bag wich i have bought and willing to trade off some of for you xtras, pm with what ya might have and what ya would like
Free Spore Ring... Feed it And Watch it GROW!
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green_guy
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Re: Glad containers [Re: pyromaniac]
#342242 - 06/16/01 02:21 PM (23 years, 7 months ago) |
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Dumb Newbie Question ---------------> What would be the big advantage of the nuking tech compared to the PF tech? All you really need is a big pot ($5 at the Goodwill) to steralize twelve glass jars which can be reused infinately after the initial cost of about $7.00 from Wal Fart.
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BrownPastures
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Re: Glad containers [Re: green_guy]
#342258 - 06/16/01 02:38 PM (23 years, 7 months ago) |
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I'm guessing the big advantage is time. In this "fast-paced" Internet world no one has the time anymore to wait for an hour till their jars are steamed. Or for that matter wait 45min till their SAFELY sterilized in a pressure cooker.:D
I personaly dont think it's the lack of time people have. I think that everyone's got that microwave sitting around and they love throwin shit in there. They see how hot stuff gets in such a short period of time and they put 1 and 2 together. More power to the people trying to figure this one out. But good luck, the packages on most microwavable food have a warning that suggested times may vary since microwave "strength" is so hard to pin down. Johny might have a Sony and Sally might have a K-mart model and Johny only cooks his food for 3min without rotating and Sally has to Cook for 5min- rotating at the 2.5min mark. So as you can see the ground is a little shifty. .. peace
"souns like your trying to teach Bruce Lee Kung-Fu"
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green_guy
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I guess the thought of killing millions of living things with radiation has thrilled mankind (or at least the Americans and Russians) for the past sixty something years.
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Nagual
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Re: Glad containers [Re: green_guy]
#342298 - 06/16/01 03:25 PM (23 years, 7 months ago) |
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If you have access to a microwave and some tupperware containers(which alot of us do), youre that much closer to making shrooms since most of us cultivation virgins don't have canning jars and pressure cookers. A hot glue-gun and some micronmagick filter discs, and like a hot nail to cut a hole in the lid would way improve air exchange; you could even use the same container to both incubate and case (for small-medium casings) But you gotta know how your microwave works.
o0O)}>there is no real death, life is a dream and we are the imagination of ourselves<{(O0o
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FLboy
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Re: Glad containers [Re: Nagual]
#424681 - 10/13/01 11:04 PM (23 years, 3 months ago) |
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i experimented with a glad container in the pc. i made a batch of white rice (for dinner-really) and the container survived fine. the rice was a bit overcooked but tastey. unfortunately the containers don't fit my pc well making good ol glass more practical.
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shroomguy
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Re: Glad containers [Re: pyromaniac]
#428321 - 10/17/01 10:19 AM (23 years, 3 months ago) |
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I was going to use those containers to case my cakes since I only want to put 1-2 cakes in each casing. Would these be good and do I have to sterilze the containers before I case my jars.
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