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Offlineauto59009
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Registered: 05/21/01
Posts: 376
Loc: Australia
Last seen: 15 years, 1 month
spore temps...
    #345701 - 06/21/01 02:53 AM (15 years, 5 months ago)

spores die at 32F right? would storing a syringe in a fridge affect viablity or germination time when warmed and used later?
i am having trouble trying to determine wether my source of error is the spores themselves or the storage area...

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OfflineNeonBlack
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Registered: 11/24/00
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Last seen: 7 years, 3 months
Re: spore temps... [Re: auto59009]
    #345837 - 06/21/01 10:13 AM (15 years, 5 months ago)

In my experience, a fridge is fine for storing spores. Are you having germination problems?



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Offlineauto59009
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Registered: 05/21/01
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Loc: Australia
Last seen: 15 years, 1 month
Re: spore temps... [Re: NeonBlack]
    #346102 - 06/21/01 06:40 PM (15 years, 5 months ago)

yup :frown:
i am planning a test to determine the cause later this afternoon.

I am a compulsive liar :wink:
http://go.to/FreeSporeRing
http://www.lilshopofspores.com
http://www.shaman-australis.com/shroom


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OfflineL3D
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Registered: 06/15/01
Posts: 273
Last seen: 11 years, 2 months
Re: spore temps... [Re: auto59009]
    #346122 - 06/21/01 07:07 PM (15 years, 5 months ago)

What substrate / setup are you useing? we maybe able to shed some mroe light for you.
Storeing if the fridge is recomended for up to 5 months but i wouldn't store them long myself




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OfflineNagual
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Registered: 06/07/01
Posts: 316
Loc: nyc, USA
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Re: spore temps... [Re: auto59009]
    #346143 - 06/21/01 07:41 PM (15 years, 5 months ago)

Hey auto, I was just reading a thread that brought up a good point;
mushrooms spores are designed to withstand climactic flux to a
much higher degree than the parent (mushroom),so if spores in
say Canada or Washington can survive a harsh winter only to pop
pop pop up again year after year than chances are theyll be OK
in the fridge for a long time. Or at least lets hope so ;)=D

o0O)}>I seem to be a verb<{(O0o
the FSR is groovalicious
!!Crobih is my founder of the Shroomery!!


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Anonymous

Re: spore temps... [Re: Nagual]
    #346240 - 06/21/01 10:10 PM (15 years, 5 months ago)

There are many mushroom species which grow in cold climes,spores and mycellium of Amanita muscaria in Siberia withstand -50 winter temps.

Psilocybe cubensis does not grow in areas that experience freezing temps, they generally come from countries close to the equator. However, I froze a test syringe and it still worked.

Ao, no, 32F will not kill your spores.

Take a trip to the Spore Lab @:
http://www.SporeLab.com
email: getspores@sporelab.com


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Offlineauto59009
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Registered: 05/21/01
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Loc: Australia
Last seen: 15 years, 1 month
Re: spore temps... [Re: ]
    #346331 - 06/22/01 12:32 AM (15 years, 5 months ago)

yeh didn't think so :smile: i have stored myc and spores that way before, thanks for all your help guys!!!
i believe it was either a non-viable print... or i ruined the print making a syringe...
i had no knife pre-sterilized coz i forgot about it (long story) so i rubbed it down with an alco-wipe and then dried it on some sterile paper... surely this would not half killed the spores? the *minute* amount of ethanol left on the knife would not even have touched all the spores. the print was a year old (maybe more) which could have affected viability i know.

any other ideas?

I am a compulsive liar :wink:
http://go.to/FreeSporeRing
http://www.lilshopofspores.com
http://www.shaman-australis.com/shroom


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