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auto59009
enthusiast
Registered: 05/20/01
Posts: 376
Loc: Australia
Last seen: 23 years, 3 months
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spore temps...
#345701 - 06/21/01 12:53 AM (23 years, 7 months ago) |
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spores die at 32F right? would storing a syringe in a fridge affect viablity or germination time when warmed and used later?
i am having trouble trying to determine wether my source of error is the spores themselves or the storage area...
I am a compulsive liar
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NeonBlack
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Registered: 11/23/00
Posts: 331
Last seen: 15 years, 5 months
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Re: spore temps... [Re: auto59009]
#345837 - 06/21/01 08:13 AM (23 years, 7 months ago) |
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In my experience, a fridge is fine for storing spores. Are you having germination problems?
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auto59009
enthusiast
Registered: 05/20/01
Posts: 376
Loc: Australia
Last seen: 23 years, 3 months
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Re: spore temps... [Re: NeonBlack]
#346102 - 06/21/01 04:40 PM (23 years, 7 months ago) |
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yup
i am planning a test to determine the cause later this afternoon.
I am a compulsive liar
http://go.to/FreeSporeRing
http://www.lilshopofspores.com
http://www.shaman-australis.com/shroom
-------------------- I am a compulsive liar
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L3D
enthusiast
Registered: 06/15/01
Posts: 273
Last seen: 19 years, 4 months
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Re: spore temps... [Re: auto59009]
#346122 - 06/21/01 05:07 PM (23 years, 7 months ago) |
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What substrate / setup are you useing? we maybe able to shed some mroe light for you.
Storeing if the fridge is recomended for up to 5 months but i wouldn't store them long myself
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Nagual
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Registered: 06/07/01
Posts: 316
Loc: nyc, USA
Last seen: 21 years, 2 months
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Re: spore temps... [Re: auto59009]
#346143 - 06/21/01 05:41 PM (23 years, 7 months ago) |
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Hey auto, I was just reading a thread that brought up a good point;
mushrooms spores are designed to withstand climactic flux to a
much higher degree than the parent (mushroom),so if spores in
say Canada or Washington can survive a harsh winter only to pop
pop pop up again year after year than chances are theyll be OK
in the fridge for a long time. Or at least lets hope so ;)=D
o0O)}>I seem to be a verb<{(O0o
the FSR is groovalicious
!!Crobih is my founder of the Shroomery!!
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Anonymous
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Re: spore temps... [Re: Nagual]
#346240 - 06/21/01 08:10 PM (23 years, 7 months ago) |
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There are many mushroom species which grow in cold climes,spores and mycellium of Amanita muscaria in Siberia withstand -50 winter temps.
Psilocybe cubensis does not grow in areas that experience freezing temps, they generally come from countries close to the equator. However, I froze a test syringe and it still worked.
Ao, no, 32F will not kill your spores.
Take a trip to the Spore Lab @:
http://www.SporeLab.com
email: getspores@sporelab.com
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auto59009
enthusiast
Registered: 05/20/01
Posts: 376
Loc: Australia
Last seen: 23 years, 3 months
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Re: spore temps... [Re: ]
#346331 - 06/21/01 10:32 PM (23 years, 7 months ago) |
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yeh didn't think so i have stored myc and spores that way before, thanks for all your help guys!!!
i believe it was either a non-viable print... or i ruined the print making a syringe...
i had no knife pre-sterilized coz i forgot about it (long story) so i rubbed it down with an alco-wipe and then dried it on some sterile paper... surely this would not half killed the spores? the *minute* amount of ethanol left on the knife would not even have touched all the spores. the print was a year old (maybe more) which could have affected viability i know.
any other ideas?
I am a compulsive liar
http://go.to/FreeSporeRing
http://www.lilshopofspores.com
http://www.shaman-australis.com/shroom
-------------------- I am a compulsive liar
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