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Anonymous

Re: Oven innoculation may have killed my spores!
    #74685 - 03/30/00 12:05 AM (17 years, 6 months ago)

you did not specify the temperature of the center core substrate in the jars at the time of innoculation.

never innoculate hot as you will kill the spores.



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Anonymous

Re: Oven innoculation may have killed my spores!
    #74687 - 03/30/00 04:23 PM (17 years, 6 months ago)

Another important question is what temperature are the jars at?

Also, the SCGT recommends flaming the needles between jars, as do many people. I find this unnecessary. Worse, if you don't cool the needle properly before innoculating you could easily kill the spores. Could this have been the problem?

A week and half is bad.. even you start to get growth it is likely to be so slow some sort of contam eventually comes in and halts growth. They may be contamed already, I've known of a fruity smelling contam that is invisible but halts mycel growth.

Did you do anything not as described by the SCGT? I consider it the most foolproof guide out there, and am surprised its not working.



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Anonymous

Re: Oven innoculation may have killed my spores!
    #74688 - 03/30/00 05:55 PM (17 years, 6 months ago)

I done the same method. Only I didn't keep the syringe in the heat long enough to really affect it. I bent down, inoculated, and brought the syringe back up to the "mainland". I'll keep you posted on the out comes. The only reason I did do it this way was, the damn syringes wasn't long enough to penitrate all the way through the vermiculite layer. If anyone this my SHEEEEITTTT is contaminated also, post it here and let me know your thoughts.
thanks


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Anonymous

Re: Oven innoculation may have killed my spores!
    #74689 - 03/30/00 06:09 PM (17 years, 6 months ago)

after sterilizing jars you should let them sit overnight or for a good few hours so they can cool down to room temperature. then inoculate, otherwise the heat still in the jars can kill the spores. then take care of setting up something to keep the jars around 86 degrees Fah. for fastest colonization.


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OfflineoOjonahOo
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Re: Oven innoculation may have killed my spores!
    #74690 - 03/30/00 10:09 PM (17 years, 6 months ago)

The jars were most definitely cool when I removed them from the pressure cooker. I had let them sit overnight and the whole day.

I did not flame sterilize, so this could not have killed them.

Contamination doestn't seem to be the problem: something happened to the spores.

Also, my syringe also wasn't long enough to penetrate the rice/verm. I ended up sticking the syringe through dry verm., then the tinfoil, then squirting the spore water throught the air onto the substrate. I didn't think this would be a problem however, as the water could visibly be seen flowing all the way down the sides. I used 1 cc per jar with 2 holes.

Another thing, the syringe was sitting in my drawer for about a month. Could this have killed the spores.It was a equadorian from Spore Chicks.

The temperature for colonization is on the cool side. In a cabinet (dark) in a chilly basement under a 400w HPS, which is busy at work growing some basil, so this makes the ambient temperature about room temperature.

Any help much appreciated.



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Anonymous

Re: Oven innoculation may have killed my spores!
    #74691 - 03/31/00 10:53 AM (17 years, 6 months ago)

There is no way that the heat from the oven is going to heat up all the water in the syringe to a thermal death temp. Even if the need (being metal and all) got that hot then the first squirt (always the best!) would cool it down.

My money is on contams or the wrong temp.

Rad



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Anonymous

Re: Oven innoculation may have killed my spores!
    #74692 - 03/31/00 05:16 PM (17 years, 6 months ago)

Maybe it was the spores :smile:... I got 4 equadorians from SporeChicks and they have been extremely slow germinators. Maybe it was a bad batch... Maybe she's reading and will send a more vibrant replacement :smile: :smile: :smile:.... heck I don't need four, one will suffice :-D


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InvisibleRyche HawkV
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Re: Oven innoculation may have killed my spores!
    #74693 - 03/31/00 07:17 PM (17 years, 6 months ago)

It sounds like you did everything right.
I am wondering what temp there stored at, under that 400W light, the temp still should be high enough to germinate.

Its very possible it was just a bad batch of spores. There isnt a spore supplier out there that hasnt sent out some non-viable syringes, I"ve read reports from all of them, including myself. Making syringes in quanity is not an easy task, and syringes are a tricky game. Sporechicks are good about sending replacements I hear, so give them a little more time, make sure your germinating temps are at least 70F minimum... 85 being more ideal. If they've been sitting for 10 days at lower temps, like in the 60's .. its a waste of time. You'll have to raise the temps and give them another week before they start to actually germinate and grow.

One thing that puzzled me a couple of you mentioned, is your needles weren't long enought to reach the substrate. Arent the syringes you got equipped with an 1 1/2" needle? That should be plenty to reach the substrate unless your using a really deep layer of vermiculite.

-peace-



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OfflineoOjonahOo
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Re: Oven innoculation may have killed my spores!
    #74694 - 03/31/00 09:44 PM (17 years, 6 months ago)

Well, i guess then that it's one of two things: nonviable spores, or the temp is too low. It is somewhat chilly down there, even with the light, so I could well be the low temp that's causing the problem. It could definitely be in the 60's. I really should get a thermometer. So if I buy a heating pad, and get the temp. up to like 80, then the mycelium will start to grow still, even after a week at the low temp?

Also, the needle looked about 1 1/2 inches. I think my mistake was that I didn't fill the jars high enough. In the Simple Cubensis Growing Tek, which was what I mainly followed, it said fill the jars with substrate up to 3/4" from the top, but I didn't know if this meant from the very top of the jar or the part where the lid starts to attach. Since, I was low on substrate, I guess I chose wrong. In the next batch, I'll make more than enough, and fill them almost all the way up.

Thanks for all the help. I'll try raising the temp and see what happens.

Oh, in the even that I can't get a heating pad, are there any other ways to get the temperature up there?



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Anonymous

Re: Oven innoculation may have killed my spores!
    #74695 - 04/02/00 03:13 AM (17 years, 6 months ago)


fish tank heater in a 1L bottle filled with water.

MC



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Anonymous

Re: Oven innoculation may have killed my spores!
    #74696 - 04/12/00 01:32 PM (17 years, 6 months ago)

blah!

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"I'd rather laugh with the sinners than cry with the saints."



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OfflineMycelium5150
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Re: Oven innoculation may have killed my spores!
    #74697 - 04/12/00 02:13 PM (17 years, 6 months ago)

why would you use an oven tek to inoculate your jars? I think it is not needed


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Offlinesutec
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Re: Oven innoculation may have killed my spores!
    #74698 - 04/12/00 02:41 PM (17 years, 6 months ago)

Actually the oven TEK is great, especially if you don't have a flow hood handy (which most people don't). If you don't have a flow hood, why WOULDN'T you use the oven TEK, unless you like contamination that is...



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Anonymous

Re: Oven innoculation may have killed my spores!
    #74699 - 04/12/00 03:26 PM (17 years, 6 months ago)

because it is unnecessary.

I'm sure you do need it for agar work and such if in absence of a flowhood.

But I've made bunches of jars, and used steam sterilization, and (cross my fingers and hope to die) have not lost a jar yet to contamination.

I'm just sure to leave the jars in the steamer for at least an hour, and I know those suckers are HOT when I take them out, I usually get burned a few times. But hey, it seems to work!



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OfflineoOjonahOo
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Re: Oven innoculation may have killed my spores!
    #74700 - 04/12/00 04:37 PM (17 years, 6 months ago)

the reason I'm not using it again is that it might have killed my spores. Probably not, since they were only subjected to the 200 degree temp for about 4 seconds, but I didn't get any mycelium, and I'd rather one or two contaminated cakes than 12 cakes with NO growth.

Maybe I'll try it again in the future if contamination is a problem.



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OfflineMycelium5150
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Re: Oven innoculation may have killed my spores!
    #74701 - 04/12/00 04:54 PM (17 years, 6 months ago)

if were talking pf jars the oven tek is very unnecessary. Thats all i was asking. Using agar or making spore syringe might be another reason, but to inoculate pf jars, waste of time.


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OfflineoOjonahOo
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Re: Oven innoculation may have killed my spores!
    #74702 - 04/12/00 05:37 PM (17 years, 6 months ago)

Okay, after three weeks, my problem has solved itself. I was right in that the temp was way too low for growth, but when I raised it too "very high" with a space heater, I didn't see any either.

Well, for whatever it's worth, now after a month since innoculation, I have little splotches of mycelium on the sides of the jars. I changed the location. Now they are on top of a heating duct. Since it's getting warm out, this may not be warm enough because it's not on too much of the time, so Im buying a heating pad later today.

Yay! White Stuff! Im not a failure!



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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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