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Re: Official Copelandia/Panaeolus Thread [Re: el boso] * 1
    #29430230 -

Also! tree frog, your description of what area of mycelium to take, from a spore plate, and subsequent plates thereon, has improved the quality of my plates tenfold. I am very grateful because I understand, now, why my cube and woodlovers plates ended up riddled with contam. Since the change from what I was doing to this better practice my contam rate has plummeted.

Thankyou very much.


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I eat a few.  should be real tender.  Its tasty.  No starchiness or crunchiness.

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Re: Official Copelandia/Panaeolus Thread [Re: el boso]
    #29430338 -

el boso said:
I have 4 AIO quart jars of Bispo Washington. I shook them 3 days ago and they're now at half colonisation. Once they hit 100% could I top fruit them? Or is it preferred to break shake and place in a shoebox?



You can top fruit them.  Probably would work better in pint or half pint jars though.

And glad the agar work is going better!


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And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster] * 1
    #29430339 -

fahtster said:
This is literally 24 hours after g2g inoculation


And it’s not even as aggressive as most ttbvi cultures I’ve found from ms.  I suspect you have bacteria in that jar or a played out culture which can happen with pan vendor lc.  Pan cultures don’t have a very long shelf life as far as expanding them goes and who knows how old that lc is.

I don’t want to bum you out but I don’t want to give false hope either.  Pans only do really well with healthy cultures.  Even the slightest bacterial culture will fruit very poorly or not at all.  It’s not looking good unfortunately



Damn! That's fast! It took 4 weeks for my TTBVI jars to colonize. I screwed up my first grow and tossed it since I didn't pasteurize my sub. I didnt see anywhere that I had to with coir and worm castings so I didnt. Anyway. Lesson learned. I Started a couple more jars yesterday. We'll see how fast they go.


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Re: Official Copelandia/Panaeolus Thread [Re: Gilmour Hendrix] * 5
    #29430595 -

current bvi mono...all my recent grows have been petites, could it be the temperature/RH thing?


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Re: Official Copelandia/Panaeolus Thread [Re: stagger]
    #29430613 -

wow thats some small ass mushrooms


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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29430638 -

yea they're tiny but have grown nicely... and will still taste splendid.  onward to other cultures.

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Re: Official Copelandia/Panaeolus Thread [Re: stagger]
    #29430696 -

and maybe extra potent


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Re: Official Copelandia/Panaeolus Thread [Re: stagger]
    #29430709 -

I think it's genetic with that line. Sometimes they're small fruits, even in better conditions.


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And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29431034 -

Bispo marco, you’re the best 🥹❤️

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Re: Official Copelandia/Panaeolus Thread [Re: philmyco]
    #29432284 -

I knocked up two jars with a bvi plate I have had in storage since October. These were done Sunday evening. I'm comparing to faht's speedy quick jars he shared. These are very slow in comparison.

I have questions.

Should I go back to spore?

Do I need better genetics?

Did I let the plate sit too long?



The condensation in the jar is likely because of the sun coming through the window to warm the jars during the day, then cooling at night.


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Re: Official Copelandia/Panaeolus Thread [Re: Gilmour Hendrix] * 1
    #29432294 -

Fahts jar was also a grain to grain.

But yeah, four days without any sign of recovery isn't good.  I would go back to germ and see if that jar shows any recovery.

Because of how long it set, it was probably in consolidation.  So it can take a bit for the mycelium to switch back to vegetation.

Still, it's taking to long imo.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29432376 -

Thanks, TF! I'll go back to the start.


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Re: Official Copelandia/Panaeolus Thread [Re: Gilmour Hendrix] * 1
    #29432490 -

My jars have hope yet. I let the agar plate get full grown then let it sit about 8 days before transfer. It might just be slower growth its starting to spread to grains that are not even touching the agar. It seems to be getting better but still super slow.

The jars are not runny with water anymore I think my heat mat makes the water come out at room temperature before I transferred there was not much water just looked wet. Anyway its drying out a little and so far I haven't noticed anything wrong except the real slow growth.


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I am the master of my fate,
      I am the captain of my soul.

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Re: Official Copelandia/Panaeolus Thread [Re: CaptainTrips420] * 4
    #29433321 -



Panaeolus cinctulus f. subsecotioides G2G>11/25/25


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In this life, trust is a luxury we cannot afford.

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Re: Official Copelandia/Panaeolus Thread [Re: CrimsonRambeler] * 2
    #29433462 -




It's like greased lightning in a jar!



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In this life, trust is a luxury we cannot afford.

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Re: Official Copelandia/Panaeolus Thread [Re: Gilmour Hendrix]
    #29434056 -

Gilmour Hendrix said:
I knocked up two jars with a bvi plate I have had in storage since October. These were done Sunday evening. I'm comparing to faht's speedy quick jars he shared. These are very slow in comparison.

I have questions.

Should I go back to spore?

Do I need better genetics?

Did I let the plate sit too long?



The condensation in the jar is likely because of the sun coming through the window to warm the jars during the day, then cooling at night.



I've had some slow jars that did ok, but that looks very sketchy.  Too wet looking to me.  Lots of burst grains.  TTBVI does great on millet and millet is so stinking easy.  Even WBS that is mostly white millet.....1 cup of dry grain, 1/2 cup water, pinch of gypsum helps with clumping.  Just dump in quart jar and straight to PC.  No prep no nothing.  2.5hrs at 15 psi and then shake a couple times (as soon as pressure drops; and once again when still warm).  TTBVI does fine on this and the larger # of inoculation points is  plus for colonization speed.


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I may not have stayed at a Holiday Inn Express, but I have taken 20 grams of fresh subtropicalis :smile:

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Re: Official Copelandia/Panaeolus Thread [Re: Hardhead]
    #29434092 -

Yeah I have noticed that a few people have good luck with no prep Millet and cyans. When I was using stipes prep millet they hated the shit.  And I had been running gourmets on it for a while.

Also popcorn isn't like other grains, bursting isn't an issue. Because they don't clump.

But yeah the jar does look too wet.  They should look more like this.  That said, that's to prevent bacteria from overrunning the jar. His wet grains aren't why the wedge isn't recovering




Edit:  And the bigger your jars the more dry your grains need to be when they go in them.  Stamets lists the moisture content and 1/2 gallon grain jars is substantially lower than quartz. Just for reference, so like I'm using little jars and if I was using quarts they would be much drier than that even


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (12/19/25 12:47 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29434445 -

tree frog said:
Yeah I have noticed that a few people have good luck with no prep Millet and cyans. When I was using stipes prep millet they hated the shit.  And I had been running gourmets on it for a while.

Also popcorn isn't like other grains, bursting isn't an issue. Because they don't clump.

But yeah the jar does look too wet.  They should look more like this.  That said, that's to prevent bacteria from overrunning the jar. His wet grains aren't why the wedge isn't recovering




Edit:  And the bigger your jars the more dry your grains need to be when they go in them.  Stamets lists the moisture content and 1/2 gallon grain jars is substantially lower than quartz. Just for reference, so like I'm using little jars and if I was using quarts they would be much drier than that even




That is good to know.  I have had popcorn stick together, but I got it waaayyy to wet.  I've got the bisporus going on popcorn.  Been running some soft white wheat but have yet to see how it is going to fruit.  Seems to colonize great in jars and no clumping, but I did do a proper prep with it.  My no prep wheat was too wet on bottom and too dry on top and ended up a clumpy ugly mess.  I threw it out.


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I may not have stayed at a Holiday Inn Express, but I have taken 20 grams of fresh subtropicalis :smile:

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster] * 1
    #29434563 -

fahtster said:
:whathesaid: or just hit up the matketplace and get some free ones

The red flag is that it’s not growing.  You can be spawning at 8 days with a healthy culture
——

Revived my MiB clone from dried grains again.  This time they were 9 months on the DG’s.  I wanted more fresh spores and they did not disappoint




The revival from dried grains for a Pan is very impressive faster!  Not that I’m at all surprised by you doing impressive things. I’ve done that dried grain thing with oysters and cubes but thought pans wouldn’t make it.

Have you posted a tek for Resurrection of cultures from dried grain spawn?

Thanks as always for all the inspiring and educational posts that you make!


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What are they saying? Listen carefully, it might be something you need to hear...




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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] * 8
    #29434607 -

Ty.  As you probably know I’ve been doing dry grains for cubes for a long time but Baba Yaga did it with pans 5 years ago.  I was like you, I thought there’s no way with how delicate pan myc is that it would revive.  Turns out it’s the fastest species to revive.  lol  whoda thought it

These grains were all dried out in July and this is only 2 DAYS on water agar

Edited by fahtster (12/19/25 10:27 PM)

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Shop: Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Myyco.com Golden Teacher Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy


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