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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #29395703 -

Still trying to get fruits. Climate is not ideal with mid 60s to low 70s and quiet dry due to wintertime.
This is pan bisporus ms: corn spawn + (coir + 10% alfala)
Casing is 100%peat pasteurized, with a bit calcium carbonate.

I see many pictures with pins here and the casing often looks very wet. But before knots appear, how should the surface conditions be? As wet?
This is sitting in a unmodified shoebox, does it look too dry? Should I mist or wait for knots?

Edited by shroboo (11/10/25 12:51 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: shroboo]
    #29395794 -

Bisporus likes it humid for pining but not wet. Tiny beads of moisture on the surface is perfect.


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Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: Official Copelandia/Panaeolus Thread [Re: phytophthora]
    #29395813 -

phytophthora said:
Almost everything posted in the shroomery is anecdotal. This doesn’t mean the methods don’t work or are incorrect. I can tell you from my own (anecdotal) observations that despite what AI tells you, and a lack of scientific studies or literature about it’s antifungal properties that E127 (red #3) is indeed antifungal. There are many posts also describing stunted, stalled, or stopped growth when it has been used. When I have transferred a lethargic sample from a plate with E137 to a regular plate of otherwise similar composition the culture did well. It may be anecdotal but afaik it’s accepted common knowledge in the OMC.


I wasn't talking down on the methods, it showed me to do my own comparisons, or stop using it... but I don't know what the common, or ai, knowledge is on it.  just thought it was relevant.

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Re: Official Copelandia/Panaeolus Thread [Re: stagger] * 2
    #29395817 -

word. sorry if I came across aggressive there. :heart:

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Re: Official Copelandia/Panaeolus Thread [Re: phytophthora]
    #29395856 -

Hi, I'm trying to get me some pans and did two jars. They started well but stalled and showed signs of bacterial contamination. I one of them part of the myc looked somewhat alive and decided to try a minigrow, sort of 1:2 (coir + manure) and cased it with verm.

That was November 2nd and now I have this




The fluffy thing doesn't look like myc but  the thing on the sides maybe

What do y'all think? Is it worth to wait or should I toss it. Thanks in advance


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Give away list

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Re: Official Copelandia/Panaeolus Thread [Re: mush_monkey] * 1
    #29395964 -

Looks like mold


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Learn it, know it, live it


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Re: Official Copelandia/Panaeolus Thread [Re: Mackload]
    #29396110 -

Hello mr tree frog. Ive been using your pan cyan agar recipe of 0.5% LME.

Im just wondering, If I got a nice clone culture that I want to store in the fridge in an agar plate poured deeply for many months, would it be better to make a higher % of nutrients or it doesnt matter.


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Edited by Anomalocaris (11/10/25 09:03 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29396333 -

I got some grain spawn of TTBVI made from 2 ml of liquid culture bought online. First time that ill grow any pan cyan. Its going so fast, after 8 days theres a huge colony allready so ill shake it tomorrow. Its like at least twice as fast as cubes!

Edited by Anomalocaris (11/10/25 11:28 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29397908 -

Anomalocaris said:
Hello mr tree frog. Ive been using your pan cyan agar recipe of 0.5% LME.

Im just wondering, If I got a nice clone culture that I want to store in the fridge in an agar plate poured deeply for many months, would it be better to make a higher % of nutrients or it doesnt matter.



My assumption is water agar at room temperature would work best for storage but I haven't tested it yet.

I do have 0.5% plates in a 50f fridge that are a few months old and doing fine though.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29397991 -

Water agar is a good idea for storage.....Kind of like doing the liquid innoculant thing with sterile water...  I've scraped old 0.5% lme agar plates that had completely dried out and had been sitting for several months at room temp and they took right off like nothing had happened. That's why I'm slow to throw my old plates away... A zero effort way of short term storage of viable genetics.

One day, I'm going to take some powder out of my jars of freeze dried stuff and put that to agar just for kicks.  Wondering if freeze drying at 125f (that is as hot as it ever gets) kills the mycelium.  Of course there are spores in there as well.  Not sterile for sure, but shouldn't be too hard to clean up if it grows.

Has anyone tried taking just a dehydrated piece of the fruiting body and growing it after it sat around a long time?


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I may not have stayed at a Holiday Inn Express, but I have taken 20 grams of fresh subtropicalis :smile:

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Re: Official Copelandia/Panaeolus Thread [Re: Hardhead]
    #29398353 -

Folks have revived dry fruits I believe.  You could probably even toss them in a bag and back in the dehydrator for an hour if you wanted to pasteurize before trying to clone.

And yeah, water agar is probably enough for long term storage.  My pans stall before reaching the edge, showing they ran out of nutrients and went dormant.

Currently using them as masters.  Take a wedge to a fresh nutrient plate, and then inoculate from that if it looks clean.  But the ones I'm doing this with is only a couple of months old.  So I'm not sure how it compares to other long-term storage methods.

I will see if I can get a clear photo I couldn't. It's really interesting to me the morphology of a fully grown-out water agar pan.  Can really see the fractals and it reminds me of a snowflake.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 7
    #29398401 -

This is what I have going on rn:



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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Official Copelandia/Panaeolus Thread [Re: PuffM4gicDr4gon] * 6
    #29398704 -


:girlofdisapproval:



:cantarguewiththat:


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Official Copelandia/Panaeolus Thread [Re: cronicr]
    #29398721 -

Godly mycelium


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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29398875 -

Kind of a weird set of bacteria but oh well


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Official Copelandia/Panaeolus Thread [Re: cronicr]
    #29398960 -

cronicr said:
Kind of a weird set of bacteria but oh well


Could you elaborate on this please?


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Official Copelandia/Panaeolus Thread [Re: PuffM4gicDr4gon]
    #29398967 -

Top plate he posted has bacteria all around the outer edge of the plate and possibly riding along on the 2nd plate based on the growth.

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Re: Official Copelandia/Panaeolus Thread [Re: DERRAYLD]
    #29398970 -

Oh snap, I thought that was clingwrap lol

Is there something specific about the 2nd picture that hints at the bacteria hitching a ride? The only difference I see is the rings, but I'm not the best at spotting hidden bacteria.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Official Copelandia/Panaeolus Thread [Re: PuffM4gicDr4gon]
    #29398974 -

The puffiness would make me take it through a cabin just to be safe.

The fact he mentioned bacteria made me think it was as well but I wouldn't be able to say definitively.

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Re: Official Copelandia/Panaeolus Thread [Re: DERRAYLD]
    #29398985 -

Thanks for taking the time to share!


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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