Auto-SummaryThe author of this post has been experimenting with liquid plant fertilizers as a substitute for manure in mushroom cultivation, specifically to accommodate their immunocompromised status and others' preferences for a more convenient and less smelly option. After a year of trial and error, they've found that a combination of organic fertilizers, such as True Organic 2-2-2 Preplant Starter and Jackpot micronute fertilizer, with a 20ml micronute dose, produces excellent results. They've successfully grown a variety of Panaeolus and Psilocybe species using this method, achieving fruit morphology similar to that of manure-grown mushrooms. The author notes that using a synthetic fertilizer, like Royal Grower, can lead to mold growth, whereas organic fertilizers have a lower contamination rate. They also discuss potential downsides, including slower colonization times for some species and the need for careful dosing due to the ease of over-fertilization.
Howdy Shroomerities. I suppose it's time to make this journal post public. Sorry for the lag, life has been lifing. Over the last year or so, I've been experimenting with liquid plant fertilizers as a substitute for manure. Now Mojave, mushrooms aren't plants. And manure is damn near free. So why even bother? Well, the reason doesn't really matter. I'm immunocompromised & handling manure isn't within my risk tolerance. Some have partners that don't appreciate having poo in the kitchen. Some just don't like having shit under their nails. Most importantly, this shit is just mad convenient.
I initially intended for this thread to open up into a larger discussion on alternatives to manure. Being that I took forever to put this up, discussion is well under way in the lovely Tree Frog's Manure is Shitty thread. So here's the quick & dirty. Although my original goal was to simply grow some pans, I've come to see this project as great way to increase the accessibility of species that benefit from nutritious substrates.
TL;DR: Organic good. 7-10ml NPK good. 20ml micronute great. Use together. Heat treatment isn't necessary, but clean spawn absolutely is. -------------------------------------------- Here's what I've been using over the past year. Royal Grower True Organic 2-2-2 Preplant Starter Jackpot micronute fertilizer
And here's how I've been using it. I've been bent on bucket tek for the sake of convenience. I think the best way to put these species on the map is by making them easier to cultivate. I personally do not find pasteurization intimidating, but plenty of folks do. So, while I recognize that proper pasteurization or sterilization would make this journey easier, I'm set on skipping them.

So, let's see some results. I've grown a fair spread of species on ferts. Here are some examples. I'll be sure to list the dose & amount of spawn used.
Panaeolus on 7ml Royal Grower, 20ml Jackpot. 1:2-1:3 ratio Pan. Cyan TTBVI, MiBR
   Pan. Cambo Jamaica, Komodo
  Pan. Olivaceus

Ps. Subtropicalis HVC on 7ml Royal Grower, 20ml Jackpot. 1:3 ratio.
    Here's a side-by-side grow. CV+ on the left, CV on the right.

Ps. Zapotecorum Talpa #2 on 7ml Royal Grower, 20 ml Jackpot. 1:3 ratio.

Ps. Mexicana Vera Cruz on 7ml Royal Grower, 20ml Jackpot, various ratios This was the first one that explicitly did not like RG CV+. MS culture, F1-F2 from wild. CV+ on the left, CV on the right. Notice the difference in fruit morphology. Same exact conditions, CV+ fruited about a week before CV.
 
Now, does it matter which NPK you choose to use? Synthetic or organic? My answer is that I haven't tried them all, but I noticed enough of a difference going organic that I think it's worth it to just start there. My contamination rate dropped from 70-80% to 30% then again to 5%. Why the 30% stall? Well, I didn't know that I had a PC issue. Turns out I was doing the majority of the work here with intermittently bacterial spawn. I figured it out around April 2025. Replacing my PC got me to 5%. Now, Royal Grower will spontaneously mold out. Don't use it. However I'm confident that contamination on true organic is a spawn/culture issue.
Pan. Cyan Burringbar (thanks 3am!) on 30ml True Organic, 20ml Jackpot. 1:4-1:5 ratio
Panaeolus on 20ml True Organic, 20ml Jackpot. 1:4-1:5 ratio Pan Cyan Burringbar
  Pan Olivaceus
 
As this was the first variety I ran on both NPKs, this grow let me know that organic ferts could lead to fruit morphology that more closely resembles fruits grown on manure. The RG grow stumped me, as I couldn't find pictures that resembled what I ended up growing. I used Kirkeng's Olivaceus grow for reference.

Ps. Subtropicalis HVC on 20ml True Organic, 20ml Jackpot. 1:3 ratio
 
Ps. Zapotecorum Talpa #2 on 20ml True Organic, 20ml Jackpot. 1:3 ratio
 
---------------- 06/2025 Update
Ps. Mexicana Chicon Nindo on 20ml True Organic, 20ml Jackpot. 1:3 ratio
 
Ps. Mexicana Vera Cruz on 20ml True Organic, 20ml Jackpot. 1:2 ratio
 
Ps. Zapotecorum Talpa #2 on 13ml True Organic, 20ml Jackpot. 1:3 ratio
 
Ps. Zapotecorum Holy Grail on 10ml True Organic, 20ml Jackpot. 1:3 ratio
 
Ps. Tampanensis Beulah on 10ml True Organic, 20ml Jackpot. 1:3 ratio
 
Pan. Cambo Sandoz on 10ml True Organic, 20ml Jackpot. 1:4 ratio
  
Pan. Tropicalis on 10ml True Organic, 20ml Jackpot. 1:4 ratio
  --------------------------------- Now for the downsides. As mentioned, this will put wonky spawn under a microscope. I think it has something to do with the lack of beneficial microbes & the ease of accessibility inherent to liquid fertilizers. The organisms at play have to work harder to extract nutrients out of compost/manure/straw/etc.
Multiple people have reported slower colonization times (compared to manure) with Panaeolus species. This aligns with my experience. Species from sect. Zapotecorum & Mexicanae fruited faster than my coir only controls. Ps. Mexicana doesn't seem to really enjoy CV+ in any way I've gone about it. Very easy to overdo it.
Leftover CV+ has a short shelf-life. Make as much as you'll use at once, and heat treat any leftovers before use.
True Organic smells like concentrated soy sauce. I think it's better than manure but you might not like it
That's about all I've got. If you've tried this out & feel I've missed something, let me know!
-MM
Edited by MojaveMyc (06/01/25 02:06 PM)
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