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Re: Spore cleaning without a centrifuge or agar [Re: Stipe-n Cap] * 4
    #29142517 -

🤣

I got everything ready to plate up. Was a hell of a busy week but I'll get to them this weekend finally and post results shortly




This bath works extremely well and I highly recommend. Literally the only time I had a bit of fluctuation was of course when I took this damn picture! Otherwise it was right on the money the whole time. I left the lid off overnight and the water evaporated, hence the unknown times in sample C and D.

I'll see how the test plates turn out and adjust temperatures and a time accordingly.

VEVOR 10L Sonic Cleaner, 304 Stainless Steel Professional Knob Control, Sonic Cleaner with Heater Timer for Jewelry Watch Glasses Circuit Board Dentures Small Parts Dental Instrument https://a.co/d/fGivb5N


Oh! Almost forgot pictures of the control plates

Edited by PhenoDreamers (03/01/25 11:45 AM)

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Re: Spore cleaning without a centrifuge or agar [Re: PhenoDreamers]
    #29142529 -

:popcorn:

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Re: Spore cleaning without a centrifuge or agar [Re: Axman] * 2
    #29143217 -

Axman said:
Update 3-1-2025:


Only one of the wild print Tico plates had anything show up, and it was on the pre pasteurized / dirty sample plate... and it looks like myc and something else :shrug:


The PESA did have a notable change though.

Dirty sample:


Pasteurized:


It's seemingly gotten rid of some of the contamination, but there is definitely something still there.

I need to pour more plates before I can do anything else.


Updated the original comment, but here are the plates after 7 days.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Spore cleaning without a centrifuge or agar [Re: Axman]
    #29143357 -

Axman said:
It's seemingly gotten rid of some of the contamination, but there is definitely something still there.

I need to pour more plates before I can do anything else.


That's not as promising as I would have hoped. Thinking about this approach and exploring my feelings on it, I realize I'm attracted to it because it offers a short process for cleaning spores which could be ready for inoculation in the course of hours. The alternative is agar, which takes over a week at least.

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Re: Spore cleaning without a centrifuge or agar [Re: tom of alchemy] * 2
    #29143503 -

Like anything else in this "hobby", dialing will be required. First attempts seldom produce optimal results. Fiddling with the time and temperature will be necessary.

Having said that, this may not even work at all (doubtful), but that won't be determined until everything has been tried. Failures tell us just as much as success, making each failure a miniature success in disguise. Should this prove to be something worth doing, it will be intended for spores mostly unworkable on agar, otherwise just use agar...or for new folks who don't have access to agar and are determined to use spores. I'm sure there's any number of applications, I won't list them all here, Y'all get the point.

@axman:

Excellent. 30 minutes definitely produced a result along the intended trajectory, perhaps an hour will clean up the remaining contaminants.

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Re: Spore cleaning without a centrifuge or agar [Re: Stipe-n Cap] * 1
    #29143638 -

So I think I can confirm that Instant pot at 45 minutes killed all spores. No contams even though I did the initial scraping in open air, but no growth.

So there is a ceiling for you!


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Spore cleaning without a centrifuge or agar [Re: Stipe-n Cap]
    #29143645 -

Stipe-n Cap said:
@axman:

Excellent. 30 minutes definitely produced a result along the intended trajectory, perhaps an hour will clean up the remaining contaminants.


With them already being subject to a bath, should I use another dirty print and run it for an hour or just run this syringe in a bath for another half hour?

I currently am PCing my agar so I should have plates tonight.

I'm also running out of picture storage on here, so I have to decide what pictures I'm going to delete before I can upload a bunch unfortunately.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Spore cleaning without a centrifuge or agar [Re: Huskies]
    #29143648 -

Instant pot under pressure or keep warm or sauté or….

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Re: Spore cleaning without a centrifuge or agar [Re: Axman] * 1
    #29143650 -

Definitely start with fresh spores and run for an hour, I'll fix your upload problem for you.

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Re: Spore cleaning without a centrifuge or agar [Re: Stipe-n Cap]
    #29143654 -

Stipe-n Cap said:
Definitely start with fresh spores and run for an hour, I'll fix your upload problem for you.


Thank you for that.

I'll grab a brother print to the one I loaded in the first syringe and give it a go.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Spore cleaning without a centrifuge or agar [Re: Axman] * 1
    #29143663 -

Remember you don't need to use the entire print in one go, just a bit goes a long way. This way you have enough for future testing. One print should get you an easy 10 x 10ml syringes, for a large print. Small prints like 5.

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Re: Spore cleaning without a centrifuge or agar [Re: Stipe-n Cap]
    #29143758 -

I primarily intended to probe if woodlover spores would survive pasteurization. Seemed like, you know, a necessary prerequisite before trying to clean them up. But eight days in my super fresh cyanescens print doesn't show any sign of life.



This was 3 hrs @ 80*C.


--------------------
No nation, no border
Fight law and order


Species grown so far

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Re: Spore cleaning without a centrifuge or agar [Re: Churpee Choozle] * 3
    #29143771 -

Well, 3 hours at 80C is definitely not pasteurization temperatures or exposure time. Guy's, this is why I recommended starting low and working through time and temperature methodically, not picking a rando temp/time.

Some folks are running agar sterilization cycles in their IP, others want to expose to constant heat overnight, and everything in between. Pasteurization takes place at 145°F/63°C for 30 mins.

30 mins seems short, 1 hour seems reasonable. Should pasteurization fail, increase time until maybe 1.5 hours. If fwikure still occurs at 145 for 1.5-2 hours, start again at 1 hour exposure with a higher temperature, but not sterilization temps. :lol:

I understand this is an open post for tinkering but it's kind of important to keep in mind how and why stuff works so that your efforts are informed and materials not wasted on hail-mary attempts at the unreasonable.

Also keep in mind that fresh spores are going to be much more sensitive to heat exposure than dried/cured spore stock. Outcomes may likely be different for different species.


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Re: Spore cleaning without a centrifuge or agar [Re: Stipe-n Cap] * 1
    #29143776 -

Ah my bad, I run my straw for eryngii for three hours, heating to 80*C, keeping it at that for an hour and then leave it, lid on, for two hours. Been doing that for so long that I don't think about the process anymore. Just threw my syringe in.
But yeah that's probably way overkill. Had issues with straw in the past. I guess I just adjusted my time until these problems passed.


--------------------
No nation, no border
Fight law and order


Species grown so far

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Re: Spore cleaning without a centrifuge or agar [Re: Stipe-n Cap] * 1
    #29143777 -

Stipe-n Cap said:
Like anything else in this "hobby", dialing will be required. First attempts seldom produce optimal results. Fiddling with the time and temperature will be necessary.


You're right. Sorry for getting discouraged. Keep doing science.

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Re: Spore cleaning without a centrifuge or agar [Re: tom of alchemy] * 2
    #29143780 -

No need to be discouraged, brotha. Lot's of time and spores out there. Every data point counts and helps build the bigger picture, just don't give up and try not to throw too many hail Mary's before Y'all run out of spores. :lol:

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Re: Spore cleaning without a centrifuge or agar [Re: The Tao] * 1
    #29143840 -

The Tao said:
Instant pot under pressure or keep warm or sauté or….


Ah good question. Pressure cooked for 45 minutes alongside agar was seemingly enough to kill all spores.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Spore cleaning without a centrifuge or agar [Re: Huskies]
    #29143849 -

Stands to reason :rofl:

Sorry breh, didn't mean to call you out on that last post :lol:

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Re: Spore cleaning without a centrifuge or agar [Re: Huskies]
    #29143956 -

No it's ok I get it. If it worked, it would be stupid to not know it. And now we KNOW.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Spore cleaning without a centrifuge or agar [Re: Huskies] * 2
    #29143960 -


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