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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Pristine BRF Puck tek [Re: ReverendMyc]
    #29038155 -

Rev - Thanks for the excellent (and timely) write-up!!!

I have some plins on some PE7 plates, time to transfer a couple and see what happens! Plus I have a number of questionable cultures as well, this will hopefully let me rescue those.

Questions:

Would a 1/4" hole in the lid with an SFD be too much GE, letting it dry out? I'd like to use some of the lids I already have, but I can certainly make some more that are just for pucks.

Also, is the PC time the same if I am using an Instant Pot?


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Pristine BRF Puck tek [Re: fahtster] * 4
    #29041968 -

fahtster said:
Aight.. giving it a shot.  The trenches were very easy to make.  Did use a butter knife.  I have two cultures that are obviously bacterial so we’ll see how it goes and I’ll post the results



Update on this..

This was the baccy yeti culture I was cleaning up.


The trench ended up being too wide for the myc to bridge from the top, but it did make its way from the bottom..


Once it was against the glass, I just used a forceps to snag some myc and transfered it to a new small grain jar and it looks 100% healthier and colonizing at a normal rate uninhibited


Also did some baccy maketi clone grains and they didn’t even take off at all on the brf.  They were ridiculously bacterial tho (user error—I was storing my lil jars in sandwich baggies to try and keep dust off em for cleaner g2g but it caused my filters to get wet.. glad I figured it out early and stopped doing that and luckily I have the culture backed up on dry grains)


Great write up, rev!

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Re: Pristine BRF Puck tek [Re: fahtster]
    #29052500 -

Ordered those little jars.
Sat down to punch holes in the lids.
And Imma throw a toddler tantrum now.
My pretty hole punch I bought long ago for making lids won't work.

And my drill is at work.

Sigh...




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Re: Pristine BRF Puck tek [Re: DontAskJay] * 1
    #29052510 -

Well that sux. Got a hammer, nail and small block of wood?


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Pristine BRF Puck tek [Re: DontAskJay] * 2
    #29052516 -

I just used a hammer and nail, put two small holes in, filter stickers top and bottom, they're colonizing great.



But also thank you Rev. This has been a great tek to run.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Pristine BRF Puck tek [Re: ReverendMyc]
    #29052543 -

ReverendMyc said:
Well that sux. Got a hammer, nail and small block of wood?


Yeah. But I'll just grab my drill tomorrow. Will go a lot faster.
Tomorrow we make Brf cakes!

Great writeup.
Thank you.

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Re: Pristine BRF Puck tek [Re: DontAskJay] * 4
    #29054334 -

Inspired by the faht himself, I’m also experimenting with trenched brf pucks. I got some new cultures I need to clean since they’ve been poppin some funny red bacterial biofilm :wow:  Ngl these 3-4 millimeter trenches were a bitch to make, the brf kept collapsing like sand, thank god I got plenty experience building sand castles as a kid :rofl: Will report later if the myc was able to cross the trench or if the distance needs to be smaller


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Re: Pristine BRF Puck tek [Re: Radamanto] * 6
    #29088880 -

Inoculated on the 5th of January with a MSS of Iceberg and DC Mac spores.

I didn't have vermiculite, so used 2 parts field capacity coir to 1 part BRF instead, they seem to be working fine:


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Re: Pristine BRF Puck tek [Re: Dkeppel] * 1
    #29088922 -

:hellyeah:


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Pristine BRF Puck tek [Re: Radamanto] * 7
    #29095329 -

I'm back after a month with what I think are super good news. I inoculated this brf puck with some x7x+ T1 on 12.13.25 and here's how it was yesterday morning:



I couldn't take pics before because of the lid, but even on these "late" pics you can see how the 3-4mm trench worked beautifully. From the beginning, the original transfer was growing rhizo, but with that fluffy overlay on top. Until it hit the trench. The myc managed to cross it but the fluff did not, the new half of the puck shows thick, dense, healthy rhizo growth imo. I don't know if the fluff was some other myc hitchhiking a ride or just an expression of bacterial presence, but I already took transfers from the healthier section and hope to get some clean growth.

Interestingly, after the original half was completely colonized and the myc had just crossed the trench, it started to pin near the transfer side. That's why there is long plin just chilling in the pics :rofl: Again, I don't know if it was some kind of pinning response to bacterial presence, or if the myc took the full colonization of that first half as pinning time.

All in all the trench was not so hard to make, 5 minutes, a knife and some patience and you can get a handy tool to leave contams or weird growth behind. I think the trick is to make a thin trench so the myc can cross it, wetting the knife a bit helps with pushing the trench walls without collapsing them ime. Anyways, thanks to Rev and Faht for the inspiration, trenched brf puck are my new go to tek :rockon:

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Re: Pristine BRF Puck tek [Re: Radamanto] * 1
    #29095829 -

Sweet thanks for the update :headbanger:

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Re: Pristine BRF Puck tek [Re: fahtster] * 1
    #29096494 -

:whathesaid:

Beautiful. Thanks for pushing forward and sharing your info. I will add some trenching info to the OP if and when I free up some time.
:hellyeah:


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Pristine BRF Puck tek [Re: ReverendMyc] * 6
    #29107789 -


Just adding some pictures to the thread for trenching. I will be testing them on some heavily baccy plates and the wedge baggies that they came from. If all goes well I will add the info to the OP.

I used a tall narrow shot glass to compress the sub slightly to help secure the walls of the trenches made with a butter knife. It looks like they held up pretty well through the sterilization cycle. I hope to put the sus cultures on them this afternoon.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Pristine BRF Puck tek [Re: ReverendMyc] * 4
    #29108378 -

Did the transfers to try and rescue some bacterial cultures. Here are some more images from the journey.


These are plates from some baggies that had traveled half way around the world. Others were clean on plates but these were clearly not. I only tried to salvage some myc from the first two plates top left and top center. I didn't see anything worth trying on these other four.
Side note that placing plates on their side has an additional advantage besides helping with condensation. As you can see the bacterial goo slides down while the myc on the first plate tried to climb up. That was pretty helpful to separate them for this attempt.


The first one from above had some separation of myc and bacteria, so I tried to get the tiniest piece and placed it near the center of the smaller half of the brf puck.


This plate had a slight bit of aerial myc coming off of the center transfer piece. I uses a loop and the scalpel to try to just get the aerial fluff.

For the rest, I went back to the original baggies to try to get some living tissue to work with. Now we wait.
:waitingpatiently:


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Pristine BRF Puck tek [Re: ReverendMyc]
    #29112760 -

Wow, you really have a thread for everything that i am interested in.

Is it possible to use brf pucks to inoculate grain-jars/bottles like you do with normal g2g? I have never grown from a spore print and i was wondering what i should focus on.

print -> agar -> muda bottle / grain
or
print -> brf pucks -> "g2g" into a muda bottle

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Re: Pristine BRF Puck tek [Re: blueberrybroccoli] * 2
    #29112768 -

I've done it a few times with varying levels of success, I recommend going back to agar then a2g but you can scrape the mycelium off of the top of the puck and drop it into grain jars.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


Edited by Axman (02/03/25 07:02 PM)

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Re: Pristine BRF Puck tek [Re: Axman] * 1
    #29112790 -

Alright, i will try both going back to agar and directly to grain. Thanks

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Re: Pristine BRF Puck tek [Re: Axman]
    #29112797 -

Edit: i just found out that this would not work

Oh and could i do this:
Fill a bottle with brf like here and inoculate with spores, let it colonize and then case it with some vermiculite and put into fruiting?

I think i saw this on mudas bottle thread. Sorry for the noob questions

Edited by blueberrybroccoli (02/03/25 07:47 PM)

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Re: Pristine BRF Puck tek [Re: blueberrybroccoli] * 2
    #29113581 -

Baba Yaga has a thread on doing this. I also think that fahtster did something similar for years before recently starting with agar. So it is certainly possible.

I would caution that using BRF doesn't eliminate bacteria. It simply keeps it from moving and spreading. So if you have a puck with hidden bacteria and then put it in grain and shake it up, you have lost that protection if you break up the puck. Keeping it whole should/could keep bacteria trapped whether sent to grain or spawned to coir. Some people shred cakes in coir, but I would trust just burying them in coir more. (edited)

What you described in the second part is basically PF Tek and probably the most reliable way to get fruits. Especially when starting out.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

Edited by ReverendMyc (02/04/25 05:43 PM)

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Re: Pristine BRF Puck tek [Re: blueberrybroccoli] * 2
    #29113810 -

Many folks use pucks to get an in-vitro pin for cloning, as they are pretty clean. Or case with coir or verm and top fruit it. I have done the latter for the purpose of getting a fruit I could print. After that, I spawned the pucks into coir in qt size PP5 screw tops.

They started pinning a couple of days ago!


Just remember that the thinner BRF pucks will have fewer resources for the fruits, but you can easily fill the jars more and make full size cakes which can be top fruited as well.


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Shop: Myyco.com Golden Teacher Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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