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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: SirPsycho] * 4
    #27918836 -

Ok. Posting on the run. Still haven't read every post yet lol. Exceptional Situation. Not really. Just started diffently than most. Homestead agar kit and before that, stealing chunks of turf, gardens and chip piles to transplant. :lol: That's all we had. No internet and few books.
Now thereโ€™s such a wealth of resources it's very likely that some are crap. Go to a good time proven vendor if you are new to this. *this message brought to you by Sporeworks*
Thinking back PF used to claim contamination resistance and superior potency with BRF. At first I was like yeah whatever. Same weird shit grows on my agar, grain and PF jars. But after trying millet it opened up a whole new world. Got me experimenting with grains in general. So I do agree that brown rice feeds less of the nasties we need to worry about. The method itself requires less sterilization work. It can even be done in a double boiler.
Anyways I guess maybe I've become comfortable with so many methods they all kind of seem the same now. :facepalm:
I can say for new people however, each grain has it's own personality. Each it's own contamination vectors. Cheap can drive you nuts so don't skimp initially. Avoid no soak teks initially. Non active bacteria are very hard to kill. Let them wake up before you try. Don't make syringes from foil prints initially. Follow the PF Tek for print to syringes.
I got a lot more but gotta run for now.

:flowerchild:

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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: the_chosen_one] * 2
    #27918873 -

the_chosen_one said:
B calm down man. I haven't even read your post yet lol.

I knew this would ruffle feathers. Don't count me out as an asshole just yet. :lol:

Give me some time to get in my door and I'll settle in here in a bit.

Just to be clear 98% is only this step. The majority of my failures come later during the fruiting stage.

BTW. I really like OP and even Ashtray. :hehehe:



Calmer than you are, dude.
Calmer than you are.

Seriously, tho, assuming that all you say is true, it got me thinking.

I mean, all day we see people squirting some syringe they ordered into microwaveable rice bags or whatever under god knows what conditions. I can't in good conscience tell them that I think squirting spores to grain is a good idea. And I'm sick of seeing people blame their failures on a "bunk syringe."

But if you're coming at it from the other side, having eliminated agar from your practice, then maybe you've really nailed down keeping all aspects of the life cycle clean to such an extent that your syringes are generally far cleaner and fresher. Just less crap in the mix over multiple generations. How long do they sit around after being made? Are you working in laminar flow? Etc., etc.


--------------------

Edited by B Traven (08/25/22 08:22 PM)

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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: B Traven] * 1
    #27918876 -

B Traven said:
the_chosen_one said:
B calm down man. I haven't even read your post yet lol.

I knew this would ruffle feathers. Don't count me out as an asshole just yet. :lol:

Give me some time to get in my door and I'll settle in here in a bit.

Just to be clear 98% is only this step. The majority of my failures come later during the fruiting stage.

BTW. I really like OP and even Ashtray. :hehehe:



Calmer than you are, dude.
Calmer than you are.

Seriously, tho, assuming that all you say is true, it got me thinking.

I mean, all day we see people squirting some syringe they ordered into microwaveable rice bags or whatever under god knows what conditions. I can't in good conscience tell them that I think squirting spores to grain is a good idea. And I'm sick of seeing people blame their failures on a "bunk syringe."

But if you're coming at it from the other side, having eliminated agar from your practice, then maybe you've really nailed down keeping all aspects of the life cycle clean to such an extent that your syringes are generally far cleaner and fresher. How long do they sit around after being made? Are you working in laminar flow? Etc., etc.



Makes me wonder how many times vendors have had to replace a "bunk syringe" when the "cultivator" was splooging them into bags of UB lol


--------------------
(You Know What Time It Is)
First they came for the communists, and I did not speak out because I was not a communist.
Then they came for the migrants, and I did not speak out because I was not a migrant.
Then they came for the queers, and I did not speak out because I was not queer.
Then they came for me, and there was nobody left to help me.

ABOLISH ICE. Free Palestine. Revolution or bust (A) . Profile pic credit goes to an awesome human
Be Gay, Do Crime 161 1312

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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: Ashtray161]
    #27918879 -

Ashtray161 said:
B Traven said:
the_chosen_one said:
B calm down man. I haven't even read your post yet lol.

I knew this would ruffle feathers. Don't count me out as an asshole just yet. :lol:

Give me some time to get in my door and I'll settle in here in a bit.

Just to be clear 98% is only this step. The majority of my failures come later during the fruiting stage.

BTW. I really like OP and even Ashtray. :hehehe:



Calmer than you are, dude.
Calmer than you are.

Seriously, tho, assuming that all you say is true, it got me thinking.

I mean, all day we see people squirting some syringe they ordered into microwaveable rice bags or whatever under god knows what conditions. I can't in good conscience tell them that I think squirting spores to grain is a good idea. And I'm sick of seeing people blame their failures on a "bunk syringe."

But if you're coming at it from the other side, having eliminated agar from your practice, then maybe you've really nailed down keeping all aspects of the life cycle clean to such an extent that your syringes are generally far cleaner and fresher. How long do they sit around after being made? Are you working in laminar flow? Etc., etc.



Makes me wonder how many times vendors have had to replace a "bunk syringe" when the "cultivator" was splooging them into bags of UB lol



Haha for real


--------------------

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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: Rotnpins] * 3
    #27918905 -

Right?

I've only come across 1 vendor syringe (from a trusted vendor) that was too dirty to successfully pull off a PF Tek grow..

I got an AA+ syringe that was extremely bacterial.. every jar looked like a vanilla milkshake with metabolites :lol: all that showed up on agar was bacteria.

Other than that, I've never seen a vendor syringe that I'd consider "bunk".. but I've read a ton of posts where someone did MSS to grain, failed, and blamed it on the syringe.. that is the target audience for this write up ๐Ÿ’ฏ

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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: Rotnpins] * 3
    #27918913 -

Spors syringes should be about the same quality as youโ€™d expect from some pre made grains bags. Never know who made it or if it was done write.

Home made is always better

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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: san pedro guy] * 1
    #27918920 -

san pedro guy said:
Spors syringes should be about the same quality as youโ€™d expect from some pre made grains bags. Never know who made it or if it was done write.

Home made is always better



:werd: home made is definitely better... but for someone doing their first grow, a vendor syringe is usually the starting point.. even if they're not the best, most of them are good enough for a successful pf tek grow..

Once someone is able to produce their own spores, vendor syringes kinda become obsolete unless it's something special and you can't seem to get your hands on it through the community

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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: Rotnpins]
    #27918937 -

Yea of course, but like said if starting out, unless you can find/live in an area with wild mushies youre not likely to have P Cube spores lying around haha. I guess you could buy a spore print and DIY the syringe but even then, same rule applies, who knows how well and sanitary the print was made lol


--------------------
(You Know What Time It Is)
First they came for the communists, and I did not speak out because I was not a communist.
Then they came for the migrants, and I did not speak out because I was not a migrant.
Then they came for the queers, and I did not speak out because I was not queer.
Then they came for me, and there was nobody left to help me.

ABOLISH ICE. Free Palestine. Revolution or bust (A) . Profile pic credit goes to an awesome human
Be Gay, Do Crime 161 1312

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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: B Traven] * 1
    #27918948 -

B Traven said:
the_chosen_one said:
B calm down man. I haven't even read your post yet lol.

I knew this would ruffle feathers. Don't count me out as an asshole just yet. :lol:

Give me some time to get in my door and I'll settle in here in a bit.

Just to be clear 98% is only this step. The majority of my failures come later during the fruiting stage.

BTW. I really like OP and even Ashtray. :hehehe:



Calmer than you are, dude.
Calmer than you are.

Seriously, tho, assuming that all you say is true, it got me thinking.

I mean, all day we see people squirting some syringe they ordered into microwaveable rice bags or whatever under god knows what conditions. I can't in good conscience tell them that I think squirting spores to grain is a good idea. And I'm sick of seeing people blame their failures on a "bunk syringe."

But if you're coming at it from the other side, having eliminated agar from your practice, then maybe you've really nailed down keeping all aspects of the life cycle clean to such an extent that your syringes are generally far cleaner and fresher. Just less crap in the mix over multiple generations. How long do they sit around after being made? Are you working in laminar flow? Etc., etc.



Cool. Cool. I like you too lol.

I'm with you. Believe me. Especially blaming syringes. I'm just saying they ain't doing it right. :lol:

Honestly out of several vendors only two have sent me provable actively dirty syringes. One doesn't exist anymore and the other worked it out. I don't buy a lot but I like to add to the collection every few years so that's to be kept in context.

It does lead me to believe the source of contamination is procedural in most cases. Next on my list would be like you said.. the grain. I'll spend the extra buck a pound. Totally worth it. I know it sucks working under a budget.. all too well. Ugh. But itโ€™s about cutting the right corners. Playing advocate for the lazy person of course. For the last several years I've been using mostly Bob's Red Mill Pearl Barley. 24 to 48 hour soak to wake up what few nasties are in there. Gravity drain until it barely drips then PC. Time depends on how much is in the jar but a minimum of 30 minutes per cup of soaked grain. I keep everything small.. mostly pint jars.
This is all with very clean well water. Never tested with city water but it's something to consider. The results might vary. Lead and fluoride concentrate as water boils. Not really shit I want in there either for several reasons.
Syringes are always made PF style with distilled water and a gram of agar flakes per gallon. PF used to do a gram per liter but it's almost a waste of agar flakes. My formula works just as well for anti-clumping imo.

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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: the_chosen_one] * 1
    #27918985 -

Ooo.. that's interesting ๐Ÿค” I've never used agar In my syringes.. I may have to try it out sometime. What is the benefit to doing this? Spores sticking more to substrate, similar to SSLC?

Honestly, I don't personally know when the next time is ill make a spore syringe tho.. I've switched over to making LC from T1 plates for my PF Tek projects while I continue to clean/isolate my plates further for grain.

This is also something I would highly recommend to those starting out with agar.. it encourages them to practice agar work and how to prepare LC, while continuing to provide reliable results during the process.

I am just getting to the point with my agar work where I might soon consider trying a batch of LC to grain (I'll probably stick to wedges anyway) however, when I do decide to give it a try, it will be a lot less intimidating and I'll probably do a lot better job since I've already had plenty of practice preparing LC for Pf Tek..

Note for those considering LC for the first time: your culture and sterile work need to be top notch before using LC to inoculate grain.. any flaw in your culture or procedures will be amplified.
Thats why I'd recommend continuing to utilize pf tek as a tool for learning. My T1 LC would probably have a really high fail rate on grain, but the contamination rates are miniscule when sending it to pf tek (I haven't lost a single jar yet)

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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: Rotnpins] * 4
    #27919003 -

We didn't have PF Tek until a few years after the Homestead kit. I got lucky and had success with my first try. That kit was awesome. $200 but it had everything but a PC and a microscope.
I believe the PF Tek is the best for beginners. It's fairly simple and it works. It's hard to put a price tag on confidence and it's the perfect tek to build it.

The agar in the water is to prevent spore clumping in the syringe. While most were playing with Jet Dry and other chemicals he was doing it like this the whole time. I actually have his hand typed recipe and instructions here somewhere. Been meaning to dig it out for the museum.

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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: the_chosen_one] * 2
    #27919014 -

the_chosen_one said:
I hate to be skeptic :lol: but I'm failing to understand how the PF Tek is less prone to contamination than a grain jar when it's the same thing. Spores going to grain. This is a great write up and I admire the passion.. knowing this took some time and effort. I guess I'm the Yang to the Yin in this case. I grow via a lot of different methods, but my most common option is spores to grain just because of the simplicity. My success rate is easily 98% making my own syringes and maybe just a little lower using vendor supplied. Like I said.. not trying to be negative. It's just this topic always leaves me a bit confused. Maybe it's because I started the long route with agar (and still use it today) but eventually worked myself back to the simple.

:flowerchild:



I agree with this statement because I also grew spores to grain with great success for many years prior to even considering agar.

On principle though I don't suggest it to new people because of the inherent risks involved, maybe we were more willing to experiment and fail in order to succeed.

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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: DERRAYLD]
    #27919059 -

I've grown multiple "strains" of cubensis from spores with spore syringes that i made with no problems.
Just used SAB that wasn't even properly made, it was in winter though, in summer i had huge problems with contamination.


--------------------
Don't worry mate, what you're gonna feel is a slight burning in your heart, your lungs, your brain, your kidneys, your liver, bottom of your feet, your eyeballs, then you start bleeding from your nose and ears, then you start tripping out and seeing everything in the form of an anime cartoon which is really fucking cool.

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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: the_chosen_one]
    #27919063 -

the_chosen_one said:
Ok. Posting on the run. Still haven't read every post yet lol. Exceptional Situation. Not really. Just started diffently than most. Homestead agar kit and before that, stealing chunks of turf, gardens and chip piles to transplant. :lol: That's all we had. No internet and few books.
Now thereโ€™s such a wealth of resources it's very likely that some are crap. Go to a good time proven vendor if you are new to this. *this message brought to you by Sporeworks*
Thinking back PF used to claim contamination resistance and superior potency with BRF. At first I was like yeah whatever. Same weird shit grows on my agar, grain and PF jars. But after trying millet it opened up a whole new world. Got me experimenting with grains in general. So I do agree that brown rice feeds less of the nasties we need to worry about. The method itself requires less sterilization work. It can even be done in a double boiler.
Anyways I guess maybe I've become comfortable with so many methods they all kind of seem the same now. :facepalm:
I can say for new people however, each grain has it's own personality. Each it's own contamination vectors. Cheap can drive you nuts so don't skimp initially. Avoid no soak teks initially. Non active bacteria are very hard to kill. Let them wake up before you try. Don't make syringes from foil prints initially. Follow the PF Tek for print to syringes.
I got a lot more but gotta run for now.

:flowerchild:



What was/is the homestead agar kit?


--------------------
(You Know What Time It Is)
First they came for the communists, and I did not speak out because I was not a communist.
Then they came for the migrants, and I did not speak out because I was not a migrant.
Then they came for the queers, and I did not speak out because I was not queer.
Then they came for me, and there was nobody left to help me.

ABOLISH ICE. Free Palestine. Revolution or bust (A) . Profile pic credit goes to an awesome human
Be Gay, Do Crime 161 1312

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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: Ashtray161] * 3
    #27919182 -



--------------------
๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ

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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: KROM] * 1
    #27919276 -

Guys, I think KROM should get one of our mushroom-power friendship rings..

He's obviously got the right attitude

:muldershroom:

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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: Ashtray161]
    #27919282 -

I could be wrong,.. but I think I remember reading somewhere that the homestead kit was a mushroom grow kit that you could order from high times magazine..

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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: Rotnpins] * 2
    #27919482 -

Rotnpins said:
Maybe I should update this to include a section about UB :shrug:



Ok, I've got something to contribute to that section:

"Contaminants can be found everywhere, which is why sterile technique is so important. One source of contamination that's not often mentioned is Reddit. It can easily infect you with brain rot."


--------------------

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Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: B Traven] * 2
    #27919486 -

Lol just wait til my Page on UB on the shroomery wiki goes live


--------------------
(You Know What Time It Is)
First they came for the communists, and I did not speak out because I was not a communist.
Then they came for the migrants, and I did not speak out because I was not a migrant.
Then they came for the queers, and I did not speak out because I was not queer.
Then they came for me, and there was nobody left to help me.

ABOLISH ICE. Free Palestine. Revolution or bust (A) . Profile pic credit goes to an awesome human
Be Gay, Do Crime 161 1312

Extras: Filter Print Post Top
Re: Spores to Grain: Why it's discouraged & Recommended Alternatives [Re: B Traven]
    #27919492 -

B Traven said:
Rotnpins said:
Maybe I should update this to include a section about UB :shrug:



Ok, I've got something to contribute to that section:

"Contaminants can be found everywhere, which is why sterile technique is so important. One source of contamination that's not often mentioned is Reddit. It can easily infect you with brain rot."




Pretty much exactly what I would have said ๐Ÿ’ฏ

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