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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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How sterile was I when I made syrinages
    #2704582 -

I am a little worried so I ask for your opinions. rate it from 1(very unsterile) to 10(pristine)

I took 7 sporeprints of different diameters and darknesses. First I filled my frying pan with cool water then I sucked the water in, out in, out to remove any traces of spores left. Then I spilled the pans water, filled it again and boiled that. I let it boil for a few min then when it stopped boiling but still extremely warm, I filled the syringe, let it sit 10 seconds then squirted it out. the plastic was very hot. I then replaced the caps for all the syringes. I spilled the water from the pan, put new water and boiled it again. as soon as it stopped boiling, I poured the extremely hot water in my glass and placed a plastic bag on it which steamed up. after letting the water cool to room temp, I opened plastic bag, used a sterile knife which was heated by a flame. I cut out the spore print, dipped it in the water. The knife was used to gentley scrape most of the spores off. I used the knife to mix the spores in the water after each spore print. Then I removed cap from syringe to suck in 12cc of sporey water. a new piece of paper towel was used to wipe the tip of each syringe before placing the cap. This all took 10-15 min. There was no air drafts and I had my face away from the glass and breathing shallowly as not to breath contams in the glass. When I looked at my syrinages again I could only see a few tiny specs of spore but the water had a very slightly purple tint but I couldnt see any more spores. Think I used enough spores? 7 spore prints measuring an inch diameter for 10 12cc syrinages

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Re: How sterile was I when I made syrinages [Re: shrooms777]
    #2704714 -

I'm sure you used enough spores, but was your print exposed to open air and for how long? That's why it's well worth it to put a glovebox together, you don't have to worry about holding your breath or anything. Anyway I certainly hope it did work out for you---only one way to find out!


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Re: How sterile was I when I made syrinages [Re: mockeylock]
    #2704765 -

id say 20-30 seconds in open air before being scrapped into the glass of boiled and sterile water. I have made syrinages before without contams but who knows whatll happen this time? so whats the rating(1-10)

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Re: How sterile was I when I made syrinages [Re: shrooms777]
    #2707085 -

If you need a number, I'll say seven. You were careful, just the open air thing.....


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Re: How sterile was I when I made syrinages [Re: mockeylock]
    #2709091 -

thanks for your opinion. bump for more opinions

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Re: How sterile was I when I made syrinages [Re: shrooms777]
    #2709927 -

You should be fine but... you don't need to keep boiling more water. Boiled spores are dead spores so who cares if a couple get in?

Just boil one pan of water. Fill and squirt like 3 or 4 times... then fill one last time.

You should be fine.


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Fiddlesticks.


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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