Home | Community | Message Board


Phytoextractum
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Jump to first unread post. Pages: 1
Offlineshrooms777
member
Registered: 05/11/04
Posts: 175
Last seen: 13 years, 1 month
How sterile was I when I made syrinages
    #2704582 - 05/20/04 07:04 AM (13 years, 2 months ago)

I am a little worried so I ask for your opinions. rate it from 1(very unsterile) to 10(pristine)

I took 7 sporeprints of different diameters and darknesses. First I filled my frying pan with cool water then I sucked the water in, out in, out to remove any traces of spores left. Then I spilled the pans water, filled it again and boiled that. I let it boil for a few min then when it stopped boiling but still extremely warm, I filled the syringe, let it sit 10 seconds then squirted it out. the plastic was very hot. I then replaced the caps for all the syringes. I spilled the water from the pan, put new water and boiled it again. as soon as it stopped boiling, I poured the extremely hot water in my glass and placed a plastic bag on it which steamed up. after letting the water cool to room temp, I opened plastic bag, used a sterile knife which was heated by a flame. I cut out the spore print, dipped it in the water. The knife was used to gentley scrape most of the spores off. I used the knife to mix the spores in the water after each spore print. Then I removed cap from syringe to suck in 12cc of sporey water. a new piece of paper towel was used to wipe the tip of each syringe before placing the cap. This all took 10-15 min. There was no air drafts and I had my face away from the glass and breathing shallowly as not to breath contams in the glass. When I looked at my syrinages again I could only see a few tiny specs of spore but the water had a very slightly purple tint but I couldnt see any more spores. Think I used enough spores? 7 spore prints measuring an inch diameter for 10 12cc syrinages


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinemockeylock
head
 User Gallery

Registered: 02/07/04
Posts: 1,046
Last seen: 7 years, 8 days
Re: How sterile was I when I made syrinages [Re: shrooms777]
    #2704714 - 05/20/04 09:33 AM (13 years, 2 months ago)

I'm sure you used enough spores, but was your print exposed to open air and for how long? That's why it's well worth it to put a glovebox together, you don't have to worry about holding your breath or anything. Anyway I certainly hope it did work out for you---only one way to find out!


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineshrooms777
member
Registered: 05/11/04
Posts: 175
Last seen: 13 years, 1 month
Re: How sterile was I when I made syrinages [Re: mockeylock]
    #2704765 - 05/20/04 10:04 AM (13 years, 2 months ago)

id say 20-30 seconds in open air before being scrapped into the glass of boiled and sterile water. I have made syrinages before without contams but who knows whatll happen this time? so whats the rating(1-10)


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinemockeylock
head
 User Gallery

Registered: 02/07/04
Posts: 1,046
Last seen: 7 years, 8 days
Re: How sterile was I when I made syrinages [Re: shrooms777]
    #2707085 - 05/20/04 06:37 PM (13 years, 2 months ago)

If you need a number, I'll say seven. You were careful, just the open air thing.....


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineshrooms777
member
Registered: 05/11/04
Posts: 175
Last seen: 13 years, 1 month
Re: How sterile was I when I made syrinages [Re: mockeylock]
    #2709091 - 05/21/04 01:40 AM (13 years, 2 months ago)

thanks for your opinion. bump for more opinions


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineRoseM
Devil's Advocate
Female User Gallery

Registered: 09/24/03
Posts: 22,294
Loc: Mod not God Flag
Last seen: 11 days, 42 minutes
Trusted Cultivator
Re: How sterile was I when I made syrinages [Re: shrooms777]
    #2709927 - 05/21/04 05:21 AM (13 years, 2 months ago)

You should be fine but... you don't need to keep boiling more water. Boiled spores are dead spores so who cares if a couple get in?

Just boil one pan of water. Fill and squirt like 3 or 4 times... then fill one last time.

You should be fine.


--------------------
Fiddlesticks.



Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Re: sterilization of spore print AnubisRonin 1,333 10 01/28/00 01:22 PM
by Anonymous
* Plastic syrinage sterilzation bjkroll 711 8 12/14/02 05:57 AM
by bjkroll
* Making syring by placing whole foil print into jar of sterilized water?
( 1 2 all )
ozzysmygod 3,473 34 01/13/09 02:55 PM
by InTheRainySeason
* I am scared to do a Print/syring! fearforce7 4,508 18 02/24/17 09:11 PM
by mushpunx
* How about this syring reuse tek Toklo 952 2 09/15/08 11:22 PM
by rugergirl79
* Sterilizing (non heat proof attributes) MushroomFriend 1,503 19 10/29/04 07:40 AM
by MushroomFriend
* Newbie about to dive in head first - amount of spore syring CCs into 4 lb grow bag?
( 1 2 all )
ChrisHippyLoser 11,476 25 06/02/16 07:47 PM
by OldSawbonesHisself
* Getting ready to make my first spore syring fromthemoon 7,022 10 06/29/07 10:49 PM
by wikedferret

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, RogerRabbit, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
668 topic views. 31 members, 108 guests and 40 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
The Best Salvia
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.008 seconds on 19 queries.