Home | Community | Message Board


Vesp.co, Inc.
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Jump to first unread post. Pages: 1
Offlineshrooms777
member
Registered: 05/11/04
Posts: 175
Last seen: 14 years, 19 days
How sterile was I when I made syrinages
    #2704582 - 05/20/04 07:04 AM (14 years, 1 month ago)

I am a little worried so I ask for your opinions. rate it from 1(very unsterile) to 10(pristine)

I took 7 sporeprints of different diameters and darknesses. First I filled my frying pan with cool water then I sucked the water in, out in, out to remove any traces of spores left. Then I spilled the pans water, filled it again and boiled that. I let it boil for a few min then when it stopped boiling but still extremely warm, I filled the syringe, let it sit 10 seconds then squirted it out. the plastic was very hot. I then replaced the caps for all the syringes. I spilled the water from the pan, put new water and boiled it again. as soon as it stopped boiling, I poured the extremely hot water in my glass and placed a plastic bag on it which steamed up. after letting the water cool to room temp, I opened plastic bag, used a sterile knife which was heated by a flame. I cut out the spore print, dipped it in the water. The knife was used to gentley scrape most of the spores off. I used the knife to mix the spores in the water after each spore print. Then I removed cap from syringe to suck in 12cc of sporey water. a new piece of paper towel was used to wipe the tip of each syringe before placing the cap. This all took 10-15 min. There was no air drafts and I had my face away from the glass and breathing shallowly as not to breath contams in the glass. When I looked at my syrinages again I could only see a few tiny specs of spore but the water had a very slightly purple tint but I couldnt see any more spores. Think I used enough spores? 7 spore prints measuring an inch diameter for 10 12cc syrinages


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinemockeylock
head
 User Gallery

Registered: 02/07/04
Posts: 1,046
Last seen: 7 years, 11 months
Re: How sterile was I when I made syrinages [Re: shrooms777]
    #2704714 - 05/20/04 09:33 AM (14 years, 1 month ago)

I'm sure you used enough spores, but was your print exposed to open air and for how long? That's why it's well worth it to put a glovebox together, you don't have to worry about holding your breath or anything. Anyway I certainly hope it did work out for you---only one way to find out!


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineshrooms777
member
Registered: 05/11/04
Posts: 175
Last seen: 14 years, 19 days
Re: How sterile was I when I made syrinages [Re: mockeylock]
    #2704765 - 05/20/04 10:04 AM (14 years, 1 month ago)

id say 20-30 seconds in open air before being scrapped into the glass of boiled and sterile water. I have made syrinages before without contams but who knows whatll happen this time? so whats the rating(1-10)


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinemockeylock
head
 User Gallery

Registered: 02/07/04
Posts: 1,046
Last seen: 7 years, 11 months
Re: How sterile was I when I made syrinages [Re: shrooms777]
    #2707085 - 05/20/04 06:37 PM (14 years, 1 month ago)

If you need a number, I'll say seven. You were careful, just the open air thing.....


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineshrooms777
member
Registered: 05/11/04
Posts: 175
Last seen: 14 years, 19 days
Re: How sterile was I when I made syrinages [Re: mockeylock]
    #2709091 - 05/21/04 01:40 AM (14 years, 1 month ago)

thanks for your opinion. bump for more opinions


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineRoseM
Devil's Advocate
Female User Gallery

Registered: 09/24/03
Posts: 22,396
Loc: Mod not God Flag
Last seen: 17 hours, 51 minutes
Trusted Cultivator
Re: How sterile was I when I made syrinages [Re: shrooms777]
    #2709927 - 05/21/04 05:21 AM (14 years, 1 month ago)

You should be fine but... you don't need to keep boiling more water. Boiled spores are dead spores so who cares if a couple get in?

Just boil one pan of water. Fill and squirt like 3 or 4 times... then fill one last time.

You should be fine.


--------------------
Fiddlesticks.



Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Sterilization of plastic jars PaC 2,867 9 10/24/03 07:00 AM
by DMJ
* Extremely Lazy Portabella/Button TEK rommstein2001 2,115 6 05/17/01 11:46 AM
by rommstein2001
* Alternative Sterilization Hoeken 599 3 06/03/01 07:50 PM
by Zeus
* Microwave Sterilization Tech? Bloomer 1,026 4 02/06/02 03:32 PM
by PowMaster2k
* Re: Microwave sterilization of casing mix Toad Man 468 1 04/06/00 09:01 AM
by Anonymous
* Need advice on sterility & trading spores. The_Gnome_King 1,769 14 06/05/01 12:16 AM
by Azure
* Plastic syrinage sterilzation bjkroll 748 8 12/14/02 05:57 AM
by bjkroll
* Sterile print? fIsh in my head 572 1 04/15/03 12:39 AM
by Dogomush

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
684 topic views. 35 members, 161 guests and 13 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
SoulSpeciosa Kratom
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.005 seconds on 19 queries.