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Offlinekbone
enthusiast
Registered: 03/29/99
Posts: 140
Last seen: 23 years, 7 months
Re: I think i got contamintion, check it out:
    #26332 - 07/31/00 12:00 PM (24 years, 3 months ago)

Using the oven will help a lot. but you should have made 2-3 holes in the lids so you can just tape them up and put the needle right in there. make sure you flame the needle with some lysol or alcohol. the first time i steralized and then put in holes after that. that's what you should do to prevent future problems :smile:

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Offlinesnarf
enthusiast
Registered: 11/22/98
Posts: 169
Last seen: 23 years, 4 months
Re: I think i got contamintion, check it out: [Re: kbone]
    #26334 - 07/31/00 02:47 PM (24 years, 3 months ago)

The real reason you want holes in the lids is so you can get air exchange going halfway through the colonization period. It speeds things up. To inject spores, you shouldn't have any contams if you use the oven tek and lift the lid off the jar just enough to get the needle in. If you use the ramsey seal however, you will be better off if you put holes in the lids. It's pretty hard to do otherwise.

I can't really comment on the steaming thing, i've never steamed jars. I hope things work out for you.

------------------
-snarf


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