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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker

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OfflineMoxemerald
Executive

Registered: 08/22/04
Posts: 390
Loc: NJ
Last seen: 7 years, 1 month
(<-noob) sterilizing the needle
    #3154057 - 09/19/04 11:05 PM (12 years, 6 months ago)

After the needle is glowing red how long do wait to make sure its cooled enough?

I cant get the PF tek to work and Im going through all the details to see what i did wrong. Ive tried it many times and havent gotten any sort of mycelium in any jar.

They all have started to smell a weird scent of fermentation/unpleasant smell each time i've tried.

I've done everything exactly as all of the guides say...I dont understand it.


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OfflineWorf
Lt. Commander

Registered: 07/04/04
Posts: 15,663
Loc: Final Frontier
Last seen: 4 years, 1 month
Re: (<-noob) sterilizing the needle [Re: Moxemerald]
    #3154076 - 09/19/04 11:09 PM (12 years, 6 months ago)

I usually flame the needle red starting at the tip then going as far as I can then making my way back up to the tip. Then just kind of squirt a little solution with the needle pointing up so that a drop forms and you can use that drop to go up and down the needle to cool it off


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OfflineMAGnum
veteran

Registered: 07/08/04
Posts: 2,421
Last seen: 5 years, 3 months
Re: (<-noob) sterilizing the needle [Re: Moxemerald]
    #3154093 - 09/19/04 11:13 PM (12 years, 6 months ago)

Wait till it cools, let it sit for a minute.


--------------------
Agent 727
7


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Invisiblez@z.com
Libertarian
Registered: 10/13/02
Posts: 2,876
Loc: ATL
Re: (&lt;-noob) sterilizing the needle [Re: Moxemerald]
    #3154161 - 09/19/04 11:26 PM (12 years, 6 months ago)

I use an alcohol wipe to cool the needle.


--------------------
"Of all tyrannies, a tyranny exercised for the good of its victims may be the most oppressive. It may be better to live under robber barons than under omnipotent moral busybodies. The robber baron's cruelty may sometimes sleep, his cupidity may at some point be satiated; but those who torment us for our own good will torment us without end, for they do so with the approval of their own conscience." - C.S. Lewis

"I would rather be exposed to the inconveniencies attending too much liberty than to those attending too small a degree of it." - Thomas Jefferson


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OfflineKaptKid
Spaced Pirate
Male User Gallery

Registered: 12/11/03
Posts: 6,248
Loc: Bright Side of the Sun
Last seen: 12 days, 40 minutes
Re: (&lt;-noob) sterilizing the needle [Re: z@z.com]
    #3154312 - 09/20/04 12:03 AM (12 years, 6 months ago)

Quote:

z@z.com said:
I use an alcohol wipe to cool the needle.




I also do. :captain:


--------------------
Child of the 60's, Tripping ever since.


:sun:


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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: (<-noob) sterilizing the needle [Re: Moxemerald]
    #3154361 - 09/20/04 12:14 AM (12 years, 6 months ago)

That fermented smell is a sign of bacterial contamination. When I was doing the PF Tek I too had a period during which every jar fell to bacterial contamination. After changing every other variable without success I finally changed the brown rice flour that I was using. That did it; I went from 100% failure to 100% success. I had been buying a bulk bin flour that was obviously contaminated.

If you are boiling your jars as I was, try changing the BR flour that you are using. Good luck.

edited for spelling


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


Edited by dog (09/20/04 01:03 AM)


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OfflineMoxemerald
Executive

Registered: 08/22/04
Posts: 390
Loc: NJ
Last seen: 7 years, 1 month
Re: (<-noob) sterilizing the needle [Re: dog]
    #3154512 - 09/20/04 12:47 AM (12 years, 6 months ago)

Thanks a lot man I will Definitley have to try.


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Offlinekonig
Stranger
Registered: 09/20/04
Posts: 6
Last seen: 12 years, 5 months
Re: (<-noob) sterilizing the needle [Re: Moxemerald]
    #3154546 - 09/20/04 12:56 AM (12 years, 6 months ago)

i use 90% rubbing alcohol and a few paper towels
easy stuff.


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Offlinecaimen
the unknown
Registered: 06/22/04
Posts: 184
Loc: HERE
Last seen: 11 years, 4 months
Re: (&lt;-noob) sterilizing the needle [Re: Moxemerald]
    #3154620 - 09/20/04 01:19 AM (12 years, 6 months ago)

make a karo jar !


--------------------
MAN Made Speed
GOD Made Weed
<Who do YOU TRUST ? >

http://www.shroomery.org/index/par/25954


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Invisibleidiotek
Registered: 02/06/04
Posts: 40,019
Re: (<-noob) sterilizing the needle [Re: Moxemerald]
    #3154699 - 09/20/04 01:40 AM (12 years, 6 months ago)

buy a pressure cooker.. so many of my PF tek problems were cured instantly for like 50 bucks at wal*mart.. 

hehe

but actually.. i used a lighter when i did the PF tek, wasn't so careful about sterile techniques.  i got some nasty green mold shit on my cakes.. one or two of them actually worked.

doubtful if that helps but uh.. yeah..  i'm just toasted.  :smile:


Edited by idiotek (09/20/04 01:42 AM)


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Offlinekonig
Stranger
Registered: 09/20/04
Posts: 6
Last seen: 12 years, 5 months
Re: (<-noob) sterilizing the needle [Re: Moxemerald]
    #3154728 - 09/20/04 01:47 AM (12 years, 6 months ago)

Hey check out my last post which shows my growth the first time. I only used rubbing alcohol to clean the needle

http://www.shroomery.org/forums/showflat...;o=&fpart=1


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Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 12 years, 3 months
Re: (<-noob) sterilizing the needle [Re: konig]
    #3154752 - 09/20/04 01:56 AM (12 years, 6 months ago)

Quote:

konig said:
Hey check out my last post which shows my growth the first time.  I only used rubbing alcohol to clean the needle

http://www.shroomery.org/forums/showflat...;o=&fpart=1


I've only been using rubbing alcohol to sterilize my needle too.

My first attempt at growing was a no-go, but not because of contams.. just.. nothing grew. My syringe was old.

All of the jars stayed sterile for 2 weeks. Slowly, they started to get contaminated.. 2/7 were still sterile(smelled good) after I finally gave up at 4 weeks.

I'm honestly not sure if the contams came from when I innoculated or what, but it seems like it would've been visable before 2 weeks if it was in there.. molds and such grow fast.

I just tried again with fresh syringes on the 18th, using the same alcohol-soaked tissue technique to both sterilize and keep the needle out of the air in between innoculations.

Hope it works. :wink:


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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