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ButterNut
Squash

Registered: 04/17/04
Posts: 1,466
Loc: Throughout the world
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Mystery contams
#2570625 - 04/17/04 10:05 AM (19 years, 9 months ago) |
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I have 2 grainbags someone gave me that were bought online.I had them sitting here for 2 a week and there were no contams.I had 2 brand new syringes from Ralphster(different strains) so I know the syringes was good. Ibrought the bags to my glovebox(already had sprayed lysol inside,ect..)and I flamed the syringe,wiped with an alchahol prep pad and injected the spores (2 1/2 CCs each bag)into the self sealing injector site,I used a different syringe in each bag. Now withen 12 hours the bags are full of Cobweb mold and I see signs of Trich.Where did it come from?I find it hard to believe that both syringes were contaminated and I used a glovebox.The bags had absolutly no signs of Contam before hand. Could the bags have been contaminated and the bad guys were just dormant?I dont know what happened.Any ideas?
-------------------- Butternut squash are softer than acorn or other types of squash, which makes them easier to cut and prepare.
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doc34
Fungitarian


Registered: 02/14/04
Posts: 2,667
Loc: Myceliaville !!!
Last seen: 8 months, 17 days
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Re: Mystery contams [Re: ButterNut]
#2570646 - 04/17/04 10:14 AM (19 years, 9 months ago) |
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probably right here-
"I have 2 grainbags someone gave me ".
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ButterNut
Squash

Registered: 04/17/04
Posts: 1,466
Loc: Throughout the world
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Re: Mystery contams [Re: doc34]
#2570656 - 04/17/04 10:17 AM (19 years, 9 months ago) |
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it wasnt just some stranger it was my good friend.He got shipped away to iraq before he could use them,thats the only reason he gave them away. And they had absolutly no signs of contams before i innoculated.I am not a noob,ive grown 10 + crops,i just never had this problem.
-------------------- Butternut squash are softer than acorn or other types of squash, which makes them easier to cut and prepare.
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doc34
Fungitarian


Registered: 02/14/04
Posts: 2,667
Loc: Myceliaville !!!
Last seen: 8 months, 17 days
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Re: Mystery contams [Re: ButterNut]
#2570662 - 04/17/04 10:19 AM (19 years, 9 months ago) |
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did you wipe the "self sealing injector site" down with alcohol?
Edited by doc34 (04/17/04 10:19 AM)
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ButterNut
Squash

Registered: 04/17/04
Posts: 1,466
Loc: Throughout the world
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Re: Mystery contams [Re: doc34]
#2570670 - 04/17/04 10:23 AM (19 years, 9 months ago) |
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yes i was absolutly sterile and did everything the way I normally do,and it was in my glovebox.They just exploded with contams withen 12 hours.and like i said it was both bags from 2 different syringes so its most likely the bags,but they showed no signs of contams thats why i thought it was so weird.As if the cobweb was dehydrated and waiting for some spore water to bring it back to life thats what it seemed like anyway.
-------------------- Butternut squash are softer than acorn or other types of squash, which makes them easier to cut and prepare.
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OJK
Stranger

Registered: 06/08/03
Posts: 10,629
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Re: Mystery contams [Re: ButterNut]
#2570684 - 04/17/04 10:32 AM (19 years, 9 months ago) |
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It's possible that the syringes were the problem; that's what it sounds like. I know that Ralphter's in one of the best, but it's worth sending him an email to ask. Also, do you know who the bags were bought from? If it was mycobag.com, or another shroomery sponsor, you should get in touch to ask about your "dormant contamination theory". If it was mycobag.com, then they should know all about this kinda thing.
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ButterNut
Squash

Registered: 04/17/04
Posts: 1,466
Loc: Throughout the world
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Re: Mystery contams [Re: OJK]
#2570741 - 04/17/04 11:03 AM (19 years, 9 months ago) |
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Well im gonna knowck up a few popcorn jars today with some of whats left out of the 2 syringes.That way If its them,ill know.Then i can contact the vendors if need be.
-------------------- Butternut squash are softer than acorn or other types of squash, which makes them easier to cut and prepare.
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TheDrugStore

Registered: 12/23/03
Posts: 694
Loc: Hawaii
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Re: Mystery contams [Re: ButterNut]
#2570854 - 04/17/04 11:43 AM (19 years, 9 months ago) |
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Quote:
ButterNut said: Well im gonna knowck up a few popcorn jars today with some of whats left out of the 2 syringes.That way If its them,ill know.Then i can contact the vendors if need be.
Good Idea.
-------------------- Penalties against drug use should not be more damaging to an individual than the use of the drug itself. Nowhere is this more clear than in the laws against the possession of marijuana and mushrooms in private for personal use.
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