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MacMerdin said:

I have been successful with the following species: Panaeolus bisporus, Panaeolus cambodginiensis, Panaeolus cyanescens, Panaeolus tropicalis and Panaeolus sect. Verricuspora using the methods below.

Panaeolus bisporus (*)



Panaeolus cambodginiensis



Panaeolus cyanescens




Panaeolus tropicalis



Panaeolus sect. Verricuspora (*)





I have used both half pint jars and Ziploc half pint plastic bowls.



Both have worked but the Ziploc bowls have more surface area to fruit.  One thing about the plastic bowls though.  The color of the lids makes it hard to see the holes that you made.  How I combat this is to add colored tape (electrical black tape works well) and then poke the holes through the tape.



Mac's Modified PF Recipe:

1 full bowl (= 1 cup) of dry vermiculite
1 full bowl (= 1 cup) of store bought manure/humus mix (I bought the Scott's brand)
1 full bowl (= 1 cup) of water
1 full bowl (= 1 cup) of brown rice flour (* for the grass loving species I substitute chopped grass seed for the brown rice)
1/2 bowl (= 1/2 cup) of worm castings
1 Tablespoon used Duncan Donuts coffee
1 teaspoon gypsum

* Makes 5 cakes with enough room for a dry verm layer

Top them off with a dry vermiculite layer as usual.

Here are photos of a colonizing Ziploc.

Day 9 after inoculation (it usually takes around 7 days to start seeing good growth for Pans IME):



2 weeks (14 days) after inoculation.



3 to 4 weeks (21-28 days) after inoculation.

 

As you notice, I keep the foil on the lids for an extra protection sometimes (not needed).  I poke air holes (after inoculation and when growth is seen) in the foil that are offset from the inoculation holes in the lids.  Plus there is a dry vermiculite layer so it's probably overkill but it works.

Once colonized...wait three days to a week consolidation time and then comes the fun part.

Take the top off the jar/Ziploc and scrape the dry vermiculite layer out.  Don't worry if some myc has grown up into it.  It will recover pretty quickly.  Just get out what comes out without hurting it too much.

Then, case the cake with 50/50 + pasteurized mix at a thickness of just enough to cover the mycelium and try to even it out as best as you can.  Don't worry too much about how even it is....it's not a big deal breaker.

Casing Recipe:

1 cup Vermiculite
1 cup Peat
1 cup boiling water
1/4 teaspoon Organic Hydrated Lime
1 teaspoon Gypsum

* Makes enough for 5 cakes with some left over....wait 24 hours to cool of course.

**  Disclaimer:  I am still tweeking this recipe and do not know how well it performs vs. other recipes.  Please use at your own risk (also if anyone has a better recipe please let us know).

I then place them directly into the SGFC after casing.  I have seen people wait 48 hours for recovery but I find it not needed.

Here is the MacMerdin twist:  I bury the jars/Ziploc bowls up to the top in the perlite.  I figured that since a SGFC works from fresh air coming up through the bottom, I would use this fresh air as my FAE source.

3-10 days after placing in the FC.



7-14 days after placing in the FC.



Times will obviously vary.

As for maintenance of the casing layer and misting/fanning:

Maintenance:

Since hearing advice from RR, I now mist the casing layer directly to keep it moist.  Apply the casing layer at field capacity.  After the casing has dried a bit, mist the casing until it starts to glisten a little and then don't moisten until you see that it has dried a bit more.  Usually 1 time a day is sufficient.

Misting and Fanning:

I mist around the SGFC so that the mist gets blown around the chamber.  Then I fan and mist the same way again.

Have fun and please feel free to ask questions or comment on how I can improve. 

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stonesun said:

Panaeolus cyanescens/Hawaii
This is #5 in the "Just As Easy As Cubes" series.
The first of the series was the Just As Easy As Cubes - Fruiting Psilocybe galindoi/ATL#7 In A Monotub,
and the second is the Just As Easy As Cubes Series - Psilocybe semperviva (P. hoogshagenii var. convexa) In A Dubtub.
The third is Just As Easy As Cubes Series - Panaeolus cyanescens/Red Down Under In A Dubtub.
The fourth is the still ongoing Just As Easy As Cubes Series - Psilocybe tampanensis In A Monotub.

Spawn, substrate and casing materials are all the same in these series.
1 quart of sterilized rye berries were used as the spawn.
It was inoculated with an agar wedge.
Colonized in 13 days.

The bulk substrate is my regular substrate what I use for my cubensis grows as well.
It consists horse manure, coir, vermiculite and gypsum (50/30/15/5) and always pasteurized for 2 hours.
In this particular grow 3 quarts were used.

Mixed the spawn with the bulk in a trashbag lined small tub. Depth about 2".
Colonization was fast, 10 days.

Applied a thin, about 1/4" of pasteurized casing layer (50/50+).
Introduced to fruiting.
The mycelium poke through the casing in a few places, partially colonized it.
First pins appeared before casing.

Fruiting temperature is ~75F.
Natural indirect daylight in a window.

Here's the dubtub


Fucker was pinning by day 10 without a casing layer...
 

Late casing applied


Fruits!
 

Some ready to harvest here
   

     

   

   

     

Nice large caps
   

   

   

   

   

Will update with printing pictures.

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stonesun said:

Panaeolus cyanescens/Red Down Under (Redspore Mutation)
This is #3 in the "Just As Easy As Cubes" series.
As you may remember the first of the series was the Just As Easy As Cubes - Fruiting Psilocybe galindoi/ATL#7 In A Monotub,
and the second is the Just As Easy As Cubes Series - Psilocybe semperviva (P. hoogshagenii var. convexa) In A Dubtub.
I've cultivated this variety of Panaeolus cyanescens before, only on a test-scale though.
You can find that thread here: Panaeolus cyanescens/Red Down Under - Small Testgrow Pictures.

Spawn, substrate and casing materials are all the same in these series.
1 quart of sterilized rye berries were used as the spawn.
It was inoculated with an agar wedge.
Colonized in 17 days.

The bulk substrate is my regular substrate what I use for my cubensis grows as well.
It consists horse manure, coir, vermiculite and gypsum (50/30/15/5) and always pasteurized for 2 hours.
In this particular grow only 3 quarts were used.

Mixed the spawn with the bulk in a trashbag lined shallow tub. Depth about 2" or less.
Colonization was quick, 8 days.

Applied a thin, about 1/4" of pasteurized casing layer (50/50+).
Introduced to fruiting.
The mycelium poke through the casing in a few places, partially colonized it.
First pins appeared just 6 days after casing.

Fruiting temperature is ~75F.
Natural indirect daylight in a window.
Misted and fanned daily until primordia formation.

Here's the tub:
   

And a day-old pins:
   

   

 

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