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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Germicidal UV lights
    #9523877 -

I've read that polystyrene petri dishes are sterilized via gamma radiation since the low melting point of the material would cause them to be destroyed in an autoclave.

I'm wondering how similar that is to the UV germicidal lights used in labs and such -- e.g. in a flow cabinet when not in use.

Germicidal lamps obviously couldn't be used to sterilize grains, but I'm wondering if they can be used to resterilize plastic petri dishes, or even liquid cultures (removing the issue of caramelizing sugars that people often bring up).  How effective would it be against mold spores and non-bacterial/viral contaminants?


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Proud supporter of the canning jar industry.

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Re: Germicidal UV lights *DELETED* [Re: archivist]
    #24724023 -

Post deleted by LizardWizard

Reason for deletion: ok

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Re: Germicidal UV lights [Re: Reblackah]
    #24724089 -

UV-c lights are not widely used even in industry. They get sold to labs as cool gimmick. It does work but its pretty pointless if your technique is proper. If you see a lab with uv-c lights in their hoods they got sold on it by a cunning salesperson. Or its in a university where they need to spend all they money they receive


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Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

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Re: Germicidal UV lights *DELETED* [Re: bodhisatta]
    #24724106 -

Post deleted by LizardWizard

Reason for deletion: ok

Edited by Reblackah (10/20/17 09:42 AM)

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Re: Germicidal UV lights [Re: Reblackah]
    #24724116 -

Uv sterilization probably won't cut it for reused plastics. Maybe it will i found this

Look up UVC sterilization of polystyrene

https://www.microbiology.ubc.ca/sites/default/files/roles/drupal_ungrad/JEMI/16/JEMI16_144-147.pdf


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

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Re: Germicidal UV lights [Re: bodhisatta]
    #24724126 -

id rather buy a ton of pp5 containers like josex.

Josex said:






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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Germicidal UV lights [Re: mushboy]
    #24724208 -

https://www.beautyhair.co.uk/products/gx4-steriliser-sterilizer-salon-equipment-tool-steriliser?variant=30388338252

you mean like with one these?

they work for surface sterilisation for tools so i would imagine petris would be fine too.


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Re: Germicidal UV lights *DELETED* [Re: bodhisatta]
    #24724493 -

Post deleted by LizardWizard

Reason for deletion: ok

Edited by Reblackah (10/21/17 05:43 AM)

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Re: Germicidal UV lights *DELETED* [Re: Reblackah]
    #24726310 -

Post deleted by LizardWizard

Reason for deletion: ok

Edited by Reblackah (10/21/17 07:04 AM)

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Re: Germicidal UV lights [Re: Reblackah]
    #24726384 -

https://micro.cornell.edu/research/epulopiscium/bacterial-endospores

"Endospores can survive environmental assaults that would normally kill the bacterium. These stresses include high temperature, high UV irradiation, desiccation, chemical damage and enzymatic destruction."

http://www.austincc.edu/microbio/2421c/ec.htm

The first sentence of this paper says E. coli is non-spore forming, and E. coli is what was used in that paper bod brought up

I don't think UV would work for us

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Re: Germicidal UV lights [Re: potnug]
    #24726508 -

Endospores aren't spores, just FYI.

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Re: Germicidal UV lights [Re: Reblackah]
    #24726534 -

They're not spores. They're fuckin monsters.

Better shield your kids eyes or they'll get nightmares.


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contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

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Re: Germicidal UV lights [Re: Mad Season]
    #24726556 -

Same for the lamp.

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Re: Germicidal UV lights [Re: Reblackah]
    #24726563 -

:lol: tis true.

Also crazy you're up to 3k posts now xD


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

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Re: Germicidal UV lights *DELETED* [Re: Mad Season]
    #24726905 -

Post deleted by LizardWizard

Reason for deletion: ok

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Re: Germicidal UV lights [Re: Reblackah]
    #24726911 -

I'll do a baby motorboat sound in front of an opened plate now, so I have a bacterial plate to test this out with. Maybe I'll fart on one too.

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Re: Germicidal UV lights [Re: Mad Season]
    #24727463 -

BTW, just noticed YOU are the one who helped me improve my agar techniques. Didn't know it was you who wrote the "improve your sterile technique" thread. Thanks for that! Got a lot of use out of it a while back!

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Re: Germicidal UV lights [Re: Reblackah]
    #24729749 -

have you ever thought about one of these?

https://www.philips.co.uk/c-p/SCF274_31/avent-electric-steam-steriliser

only takes 8 mins.

i use for tweezers, scalpels ect just not sure how you will take the petris out and remain 100% sterile as they will have to be in halfs to surface sterilize.


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Re: Germicidal UV lights *DELETED* [Re: mustangbob3]
    #24730626 -

Post deleted by LizardWizard

Reason for deletion: ok

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Re: Germicidal UV lights [Re: Reblackah]
    #24731238 -

:laugh2: yeah was late

wasnt thinking


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