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Offlinebenjamin
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Registered: 01/05/04
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Last seen: 13 years, 4 months
Extracting mycelium from rye jar
    #2397777 - 03/03/04 11:43 AM (13 years, 9 months ago)

What i want to do is somehow extract a liquid mycelium culture from a rye jar. I was thinking I could open a colonized jar and scoop out a couple spoonfuls then fruit the rest. How should i go about sterilizing the colonized substrate and the liquid culture i'm going to use. Does peroxide kill mycelium? If it doesn't could i just put the colonized substrate in a diluted peroxide mixture and suck up the mycelium? I know i could do this just by injecting the colonized jar with some sterile water/karo and then sucking it back out after a few shakes. But the thing is these jars have crappy vent-filter filters, a bad idea to be sure. So what I'm afraid of is when i'm puncturing the filter contams trapped in the filter may get mixed in with the culture. So what i'd like to do somehow is use a couple spoonfuls of the substrate like i said, and sterilize that in a separate jar and use that for my liquid culture. Anyone have advice?


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Offlinebenjamin
da shroom

Registered: 01/05/04
Posts: 110
Last seen: 13 years, 4 months
Re: Extracting mycelium from rye jar [Re: benjamin]
    #2398211 - 03/03/04 02:04 PM (13 years, 9 months ago)

let me rephrase, with the same above objective in mind will this procedure work:

-remove a couple spoonfuls of colonized rye
-inject 10 cc's of peroxide into a jar full of pre-sterilized water (say 200cc's of water)
-drop the spoonfuls into the jar and seal
-shake the jar and suck out the floating mycelium

should i add more or less peroxide, or make any other alterations to this procedure? and come to think of it, peroxide doesn't kill mycelium correct? could i use just pure h202 or a pretty high concentration of h202 and water?


Edited by benjamin (03/03/04 02:05 PM)


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InvisibleJohn
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Registered: 08/08/03
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Re: Extracting mycelium from rye jar [Re: benjamin]
    #2398221 - 03/03/04 02:09 PM (13 years, 9 months ago)

read up on grain to grain transfers or if you must have a liquid culture i would make up some karo (or honey) water, p.c. for 20mins. then just drop a spoonful of colonized grain into the jar and let it incubate a few days then use like any other liquid culture. you have to do all this in a glove box, flowhood, or oven tek cuz opening the jar like that and exposing the rye so much your bound to get contams.


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Offlinebenjamin
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Registered: 01/05/04
Posts: 110
Last seen: 13 years, 4 months
Re: Extracting mycelium from rye jar [Re: benjamin]
    #2398229 - 03/03/04 02:11 PM (13 years, 9 months ago)

the peroxide wouldn't kill those contaminants?


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InvisibleJohn
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Re: Extracting mycelium from rye jar [Re: benjamin]
    #2398256 - 03/03/04 02:18 PM (13 years, 9 months ago)

yeah to an extent but you still wanna be careful, doing all that stuff with syringes and water is unnessary, all you have to do is spoon out some rye and dump it into and uncolonized jar and shake in a clean enviroment and your good to go.


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Invisiblebaltazar
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Registered: 02/23/04
Posts: 746
Re: Extracting mycelium from rye jar [Re: John]
    #2398270 - 03/03/04 02:21 PM (13 years, 9 months ago)

what is peroxide actualy use for in mushroom cultivation ? Why do we use it ? Can someone explain a bit ?


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Offlinebenjamin
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Registered: 01/05/04
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Last seen: 13 years, 4 months
Re: Extracting mycelium from rye jar [Re: benjamin]
    #2398275 - 03/03/04 02:25 PM (13 years, 9 months ago)

yea i guess, the jars are just really nasty, i wanted another step in between removing the colonized grain and transplanting it wherever, because i'm quite sure i can't avoid getting contams from the vent-filter filter on the grain i'm removing. Could i just put the piece i take out and put it in a peroxide bath for a bit and then in a glove box take the piece out of the bath and drop it into my new substrate?


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InvisibleJohn
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Re: Extracting mycelium from rye jar [Re: benjamin]
    #2398294 - 03/03/04 02:32 PM (13 years, 9 months ago)

try! i know mycellium will be fine soaked overnight in a 10:1 water/h2o2 solution, never tried higher than that but i think it would be okay even if it was undiluted 3% if you just kept it in there for a few minutes.


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There's a thin line between sanity and insanity... and I just snorted it.


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InvisibleJohn
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Re: Extracting mycelium from rye jar [Re: baltazar]
    #2398306 - 03/03/04 02:35 PM (13 years, 9 months ago)

it stops spores from germinating, helpful to stop mold from germinating but not to effective if it has already.


--------------------
There's a thin line between sanity and insanity... and I just snorted it.


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