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Offlinellamaboy
the weasel thatsnagged the bee

Registered: 11/08/03
Posts: 563
Loc: Portland PNW
Last seen: 14 years, 5 months
Re: Kyro Tek question... [Re: simplemachine]
    #2366427 - 02/22/04 12:23 AM (20 years, 1 month ago)

do you know if the karo/honey tek would work with pan cyans??? would they germinate in just a sugar solution, and then i could use it to innoc some manure/compost? or would there need to be some sort of manure/compost extract in the karo/honey tek. has anyone tried this?

of course pan. cyan cultures can be started on PDA right? so if they can grow on PDA, then they should be able to just use a regular karo/honey tek.

i don't know about pan. cyans i'm a newb to them, i just recieved a phatty print, and i wanted to get a good isolate on some agar, and then transfer this to some honey water to get a lot of the isolate.

i just wanted to know if that would work.

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Invisiblesimplemachine
Manfly
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Registered: 09/14/03
Posts: 1,981
Re: Kyro Tek question... [Re: llamaboy]
    #2366491 - 02/22/04 12:42 AM (20 years, 1 month ago)

I've heard that it works...


SM


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InvisibleFety
Stranger

Registered: 01/31/04
Posts: 18
Re: Kyro Tek question... [Re: simplemachine]
    #2366650 - 02/22/04 01:35 AM (20 years, 1 month ago)

ok fuck what you just read...it's whacked! don't shoot 3cc's in your Karo!

Make sure you do up 100ml of purified water and 1 teaspoon of Karo. Use a solid lid for your 1/2 pint jar. No innoc hole needed. Get it on as tight as you can. PC for 15 mins. GRADUAL HEAT! you don't want it to carmelize or it'll cause mutants. Let it cool, obviously! Your Karo jar must be clear, not cloudy, if not, do it again. In a glove box, shake the shit out of your syringe and shoot a few drops of what you have left. YES, drops! There are still zillions of spores left in your syringe. If you shoot too much the spores will compete and eat up all the nutrients and it will stall. TRUST ME! Incubate at 86 degrees and you should see some white stuff by 2nd or 3rd day. Anything other than white is a contam. Good luck!

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Invisiblesimplemachine
Manfly
Male User Gallery

Registered: 09/14/03
Posts: 1,981
Re: Kyro Tek question... [Re: Fety]
    #2366906 - 02/22/04 03:25 AM (20 years, 30 days ago)

Hmmm I didn't think that would be an issue. I figure more spores = more mycellium. Since spores aren't in tight supply for most growers I assume most use considerably more than that with out problems.

Certainly using a few drops conserves more, but by the time the mycellium is competing for resources its ready to use! It doesn't really make sense...


SM


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