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Offlinecoop
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Registered: 01/19/04
Posts: 189
Last seen: 11 years, 6 months
Kyro Tek question...
    #2355635 - 02/19/04 06:14 PM (12 years, 9 months ago)

I saw a thread on this topic a few days ago that got a lot of attention. I was curious, what is the main purpose of this tek? From the looks of the thread content, it's used to make syringes. Am I wrong here, what's the main function of the tek? Just curious...thanks!


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InvisibleImOver18
FormerlyMr.Sleep

Registered: 10/05/03
Posts: 763
Re: Kyro Tek question... [Re: coop]
    #2355641 - 02/19/04 06:15 PM (12 years, 9 months ago)

It's to make live mycelial syringes from what I understand, this enables faster colonization of the substrate you inoculate because the mycelium is already germinated and growing.


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InvisibleImOver18
FormerlyMr.Sleep

Registered: 10/05/03
Posts: 763
Re: Kyro Tek question... [Re: ImOver18]
    #2355645 - 02/19/04 06:16 PM (12 years, 9 months ago)

Or, rather the spores are already germinated and growing. You get the idea.


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InvisibleMoNk
pupil

Registered: 02/08/04
Posts: 134
Re: Kyro Tek question... [Re: ImOver18]
    #2355658 - 02/19/04 06:18 PM (12 years, 9 months ago)

as well it multplies your remaining spore solution quite alot. You can turn 1-2cc into 100-200cc of injectable solution.


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Offlinellamaboy
the weasel thatsnagged the bee

Registered: 11/08/03
Posts: 563
Loc: Portland PNW
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Re: Kyro Tek question... [Re: coop]
    #2355678 - 02/19/04 06:21 PM (12 years, 9 months ago)

karo/honey water teks are one of the greatest things that a mushroom grower can do...by just adding a few spores to your liquid, you can obtain a multitude of mycelium, which can then be sucked into a syringe and used to innoculate your favourite substrate...i use the honey water tek, and i've always had great results...i usually inject about 3cc's per quart jar of millet, and i get complete colonization in about 6-8 days.  it's just simply amazing.

and once you have your karo/honey tek going...all you have to do is transfer some of the mycelium from your first jar of liquid innoc, into another and you can have multiple never ending supplies of liquid innoc.

it's a great thing :laugh:


ahhhh...but now my question...does anyone know if karo/honey would work with pan. cyans?  what if i were to add some manure to the mix, do you think that would cuase mycelia to grow?  or is a liquid tek of pan. cyans simply out of the question.


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Offlinecoop
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Re: Kyro Tek question... [Re: llamaboy]
    #2355991 - 02/19/04 07:32 PM (12 years, 9 months ago)

Sweet...I need to try this then because my first growth is taking 3 weeks to colonize. So, I have 3ccs of this one strain left in a syringe, can I turn that into a larger amount using this tek?? Also, I could just use the entire syringe no matter how much is created to use on my jars?? The more solution the faster colonized??


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Invisiblesimplemachine
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Registered: 09/14/03
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Re: Kyro Tek question... [Re: coop]
    #2356037 - 02/19/04 07:43 PM (12 years, 9 months ago)

3 cc is plenty to colonize your liquid culture...

I wouldn't use more than 3-5 cc of myc solution to colonize a 1/2 pint jar, for fear of throwing off the water content. 3cc is plenty and if done right you should see growth overnight, since you don't have to wait for innoculation.

Coop 3 weeks is not long for a pf jar (it IS for WBS). If you really want them to grow faster check this out....

Shakeable pf tek --->http://www.shroomery.org/forums/showflat...;o=&fpart=1


SM


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OfflineTheMAJ
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Registered: 02/09/04
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Re: Kyro Tek question... [Re: simplemachine]
    #2358906 - 02/20/04 12:35 PM (12 years, 9 months ago)

If I wanted to do a crude extraction w/ Kyro tek, never fruiting, could I?


Edited by TheMAJ (02/20/04 12:36 PM)


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Offlinecoop
The Rookie

Registered: 01/19/04
Posts: 189
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Re: Kyro Tek question... [Re: simplemachine]
    #2359396 - 02/20/04 02:58 PM (12 years, 9 months ago)

I've read your tek before actually.  It will be 3 weeks exactly tomorrow that I innoculated my jars.  Like I said, they are probably only about 1/3 of the way colonized.  It's cool though for my first grow, I just need to wait this one out and find a faster way next time.

I'm sure in the next week or so, once these are colonized, I'll have more questions again.  I bought five 5x5x2in plastic containers, vermiculite, and coco coir to case my six jars within.  They will be inside a clear rubbermaid container 13in tall, on a layer of perlite.  SO, soon enough...be prepared for questioning :smile:  I gotta get this one right since I waited this long lol.

Oh, and btw, I still don't get the purpose of the Kyro Tek...ha!!  It makes liquid culture overnight correct??  Is this culture used to create spore syringe or used to fruit mushrooms directly??  I'm confused.


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InvisibleMoNk
pupil

Registered: 02/08/04
Posts: 134
Re: Kyro Tek question... [Re: coop]
    #2359428 - 02/20/04 03:08 PM (12 years, 9 months ago)

you create a mycelium spore syringe to inject into your substrate, so there is no germination required it just contiunes to grow in the substrate you put it in and colonizes alot quicker. Also its used to make a little bit of spore solution into alot.


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Invisiblesimplemachine
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Re: Kyro Tek question... [Re: coop]
    #2360176 - 02/20/04 05:47 PM (12 years, 9 months ago)

it's not really overnight...more like a week or so...

And yes the mycellial syringe is used to innoculate substrate jars and grow caps.

The Maj- Your question is actually several debatable questions in one. The main problem being that before fruiting there doesn't APEAR to be a useable quantity of alkoloids. I say apear because I have never seen solid evidence either way.

There is a tek about doing just this in the liquid cluture teks archive, but it's just an idea...I don't think the guy who wrote it ever tried it. :smirk:

Long story short if it IS feasible you would need a LOT of mycellium, knowledge, and equipment/chemicals. It is WAY easier to just grow mushrooms.



SM


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OfflineTheMAJ
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Re: Kyro Tek question... [Re: simplemachine]
    #2360264 - 02/20/04 06:12 PM (12 years, 9 months ago)

Has anyone ever read that book by Adam Gottlieb? It describes a way to get a continuos yield of 5000 doses a week. I have a greenhouse where I'm growing a good bit, but I've also got closet space which is better suited for something a little smaller.


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Invisiblesimplemachine
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Re: Kyro Tek question... [Re: TheMAJ]
    #2360324 - 02/20/04 06:24 PM (12 years, 9 months ago)

^^^ that is the one I was talking about, and I don't think it would work.


SM


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Offlinecoop
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Registered: 01/19/04
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Re: Kyro Tek question... [Re: simplemachine]
    #2360397 - 02/20/04 07:01 PM (12 years, 9 months ago)

Ok...lemme get this straight. I have 3cc's leftover from the jars I innoculated a few weeks ago. I can take these 3cc's, use the kyro tek, produce a large amount of mycelium spore solution, and then innoculate some substrate with the solution...which colonizes much quicker?? Damn, I gotta try this soon.

So, I have 6 1/2-pint jars left unused at the moment, along with all of the supplies to do the PF Tek. Using this 3cc's to create this larger amount of mycelium solution, how much of it would you use to innoculate the remaining jars?? Furthermore, say I didn't use up all of the solution I created. What can you do with it?? Can you store it in a fridge in the plastic container, or will it go bad?? Thanks!!


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Invisiblesimplemachine
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Re: Kyro Tek question... [Re: coop]
    #2360410 - 02/20/04 07:09 PM (12 years, 9 months ago)

Quote:

Ok...lemme get this straight. I have 3cc's leftover from the jars I innoculated a few weeks ago. I can take these 3cc's, use the kyro tek, produce a large amount of mycelium spore solution, and then innoculate some substrate with the solution...which colonizes much quicker??




bingo

Quote:

how much of it would you use to innoculate the remaining jars??




about 3cc and then shake it up good. you should see some fuzz growing in ~24hrs.

Quote:

Furthermore, say I didn't use up all of the solution I created. What can you do with it?? Can you store it in a fridge in the plastic container, or will it go bad?? Thanks!!




yessir, thats what you do. Or if you're feeling randy, make up another 5 or 10 liquid culture jars and noc those up with the solution from jar 1. You'll be swimmin in the stuff.


SM


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Offlineguri
Master of theimprobablitydrive

Registered: 01/10/04
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Re: Kyro Tek question... [Re: simplemachine]
    #2360492 - 02/20/04 07:38 PM (12 years, 9 months ago)

ok i have a question as well, so with the mycelium spore solution i make how long can that sit around? as long as syringes do or shorter? if so will refrigration expand the amount of time it can sit around?


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Invisiblesimplemachine
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Re: Kyro Tek question... [Re: guri]
    #2360509 - 02/20/04 07:45 PM (12 years, 9 months ago)

Well it can "sit around" untill the mycellium runs out of sugar to eat and starts to die, not as long as a spores for sure. A mycellial culture could last several months at least in the fridge, because the cold temprature slows down the growth of the mycellium.


SM


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Offlinecoop
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Registered: 01/19/04
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Re: Kyro Tek question... [Re: guri]
    #2360514 - 02/20/04 07:46 PM (12 years, 9 months ago)

Yeah, I'm wondering the same thing. If you do make a lot of solution, which would be in one of those plastic containers, how long can it stay in that container in the fridge?


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Offlinecoop
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Re: Kyro Tek question... [Re: coop]
    #2360516 - 02/20/04 07:47 PM (12 years, 9 months ago)

What do you mean when you say "make culture jars"?


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Invisiblesimplemachine
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Re: Kyro Tek question... [Re: coop]
    #2360533 - 02/20/04 07:52 PM (12 years, 9 months ago)

I mean make a container with karo and water and innoculate it like you have done with the plactic container. Most people use jars for this...the plastic container thing is just a recent fad.


SM


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