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OfflineRohypnol
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Registered: 08/04/03
Posts: 267
Last seen: 1 year, 5 months
Day 6, no mycellium.
    #1847767 - 08/25/03 10:34 AM (13 years, 4 months ago)

Argh this is pissing my friend off. He innoculated into 1/2 pint pot steralized brf jars (1/2 brf, 1/2 verm, as KusSh recommended). The jars were covered with tin foil and placed into individual boxes for lightbulbs to make sure they were kept in the dark. The boxes + jars were then moved around but always kept at an alright temperature. For a couple of days the temp was around 85, then it fell back to 73. I'd say the average temperature is about 77.

Anyway, its been 6 days and he still doesn't see any mycellial growth. He is in the process of building an overly elaborate glovebox, and he ordered a pressure cooker. He is planning on using popcorn for his next attempt.

There is no contamination in the jars either (or at least no visible contamination).

Grr, maybe this is because sporebank.com sent him non-viable syringes. They're not answering his complaint either...


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OfflineSam_Kohui
if you onlyknew...

Registered: 08/23/03
Posts: 140
Last seen: 9 years, 2 months
Re: Day 6, no mycellium. [Re: Rohypnol]
    #1847791 - 08/25/03 10:47 AM (13 years, 4 months ago)

did he shake the syringe before innoculating?? temperatures in the 70s will slow mycelial growth...in addition to building that glovebox he should build an incubator as well...give it another 8 days before he trashes the cakes...and order from a shroomery sponsor next time...


--------------------
The PF Tek...it's a piece of cake (the BRF kind :cool: )


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OfflineRohypnol
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Registered: 08/04/03
Posts: 267
Last seen: 1 year, 5 months
Re: Day 6, no mycellium. [Re: Sam_Kohui]
    #1847796 - 08/25/03 10:49 AM (13 years, 4 months ago)

Yeah he shook the syringes but not very hard because he misunderstood the importance of shaking them. It sounded like dirty air was getting sucked into the syringe when he shook it so he stopped. He should have seen SOME germination though, shouldn't he have?


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OfflineSam_Kohui
if you onlyknew...

Registered: 08/23/03
Posts: 140
Last seen: 9 years, 2 months
Re: Day 6, no mycellium. [Re: Rohypnol]
    #1847809 - 08/25/03 10:58 AM (13 years, 4 months ago)

http://www.shroomery.org/index/par/23279

Quote:

Why haven't my jars shown any growth yet?
------------------------------------------------------------------------

First mantra of the hobby - "Patience." Germination can occur as early as 2 -3 days, but it may take up to a week or more for he first signs of germination to show. It depends on a multitude of factors - some of which are: temperature, substrate, spore concentration, age of the spores, etc. There are so many different relevant factors it is very hard to determine within the 1st2 weeks why you have not shown germination. Give it at least a week before you worry, and at least 2 weeks before you panic. All you need are 2 spores to germinate and take over the entire jar - and luck is on your side when proper procedures are followed. Please be patient and do not panic after only 2 days, no matter how fast you've read other people's colonization was. Their setup, factors, everything varies just slightly from yours. Now, after a period of time with no germination, analyze all of the possibilities (such as temperature, water content, sterilization procedures, etc.) that would hinder growth. This is your best bet on learning the life cycle, and also learning the peculiarities of your personal setup (as far as variations go).




--------------------
The PF Tek...it's a piece of cake (the BRF kind :cool: )


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OfflineRohypnol
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Registered: 08/04/03
Posts: 267
Last seen: 1 year, 5 months
Re: Day 6, no mycellium. [Re: Sam_Kohui]
    #1847835 - 08/25/03 11:10 AM (13 years, 4 months ago)

Good tips. He is going to wait until next friday to order more syringes if I see no growth. He really doesn't want to spend another 15 bucks :frown: Hopefully by that time sporebank.com will send him a replacement


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InvisibleTremor1127
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Registered: 08/10/03
Posts: 3,403
Loc: In a Van Down By the Rive...
Post deleted by Administrator [Re: Rohypnol]
    #1847955 - 08/25/03 12:31 PM (13 years, 4 months ago)



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OfflineIMTRIPPINOUT
newbie
Registered: 07/30/03
Posts: 37
Last seen: 13 years, 3 months
Re: Day 6, no mycellium. [Re: Tremor1127]
    #1848281 - 08/25/03 02:36 PM (13 years, 4 months ago)

ya ya sporebank has shitty syringes i ordered 1 syringe from them of treasure coast and it took two weeks and several threatening e mails before i got my syringes they sent me two penis envy syringes and two mazatapec syringes two weeks after innoculating jars nothing ive been emailing to get replacements but im scared it will just be a waste of time and its really frustrating .i went ahead and ordered another syringe from shamanshop.com and five days later bam mycelium growth in every jar so i suggest ordering from one of the sponsors ralphsters or shamanshop.i hate sporebank!!!!!!!!!!!!!!!!!!!!!


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OfflineIMTRIPPINOUT
newbie
Registered: 07/30/03
Posts: 37
Last seen: 13 years, 3 months
Re: Day 6, no mycellium. [Re: IMTRIPPINOUT]
    #1848283 - 08/25/03 02:37 PM (13 years, 4 months ago)

thats after three days of shipping so a total of 8 days after ordering i had growth


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InvisibleTremor1127
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Post deleted by Administrator [Re: IMTRIPPINOUT]
    #1848315 - 08/25/03 02:48 PM (13 years, 4 months ago)



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Offlineacidhead1279
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Registered: 03/01/03
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Re: Day 6, no mycellium. [Re: Tremor1127]
    #1848346 - 08/25/03 03:00 PM (13 years, 4 months ago)

too bad TLG's gone...


--------------------
Farewell FRSE!  We had such good times together.


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OfflineRohypnol
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Registered: 08/04/03
Posts: 267
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Re: Day 6, no mycellium. [Re: acidhead1279]
    #1850908 - 08/26/03 11:31 AM (13 years, 4 months ago)

Day 7, Slight mycellial growth at the bottom of one of the jars :smile: :smile: 


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Invisiblebotanisthype
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Registered: 07/31/03
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Re: Day 6, no mycellium. [Re: Rohypnol]
    #1850963 - 08/26/03 11:56 AM (13 years, 4 months ago)

yeh at 70s ..those 1/2 pints germinate mad sloW!....if YOu scared of wasting spores..even if there shitty quality(spore bank /sporeworks)which are tech .the same..just use the karo tek.....1/2 or 1cc would DO it .>And after 5 dayz You get a ball of myco,ready to inoculate  30 jars ...IM doin that witH all My syringes .for back up....
karo tek
100 ml. water
2 tbsp. of karo
        im using a half pt with foil under the rim,make sure you screw tight and no water leaks,some time the foil can be cut from the rim screwinG in.too tight,no lid,so i can just poke a hold thru the foil and tape over, but alternatives are sufficient as well (picklejars etc.)have oNe hole on the lid for inoculation ..p.cook for 20-30 min...let cool and SHOOt it UP >>you should see something .within 3 dayz >under appropriate incubation! HANg in there,
it can also be the substrate mixture condition  that accelerates mYcelium growth ,i got myco growth from gt's on the 3rd day..creepers took 5, patience is a burden ,but can also be your best friend! :syringe: :syringe: :syringe: :syringe: :syringe: :syringe: :syringe:


--------------------
Please +REP if i've been of any help... thanks!!!

Dinosaur of the Funny Family, though I have short arms, they fit in my GB... lol


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OfflineSenovio
newbie
Registered: 07/26/03
Posts: 43
Last seen: 9 years, 11 months
Re: Day 6, no mycellium. [Re: botanisthype]
    #1851215 - 08/26/03 01:33 PM (13 years, 4 months ago)

Man, my friend ordered hawaiian tes syringe from sporeworks, and had insanely healthy looking mycelium. And he inoculated jars 3 weeks apart.


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OfflineIMTRIPPINOUT
newbie
Registered: 07/30/03
Posts: 37
Last seen: 13 years, 3 months
Re: Day 6, no mycellium. [Re: Senovio]
    #1851244 - 08/26/03 01:49 PM (13 years, 4 months ago)

i wish i was so lucky maybe i will try the replacements!


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Invisiblebotanisthype
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Re: Day 6, no mycellium. [Re: IMTRIPPINOUT]
    #1851280 - 08/26/03 02:05 PM (13 years, 4 months ago)

i ordered ..3 syringes ..and >.2 didint work and was mad week..i >bitched about it and they sent me my replace ments and a extra one...but karo is the only way YOu can make those least viable spores to healthy steroid monsters.LOl


--------------------
Please +REP if i've been of any help... thanks!!!

Dinosaur of the Funny Family, though I have short arms, they fit in my GB... lol


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OfflineRohypnol
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Registered: 08/04/03
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Re: Day 6, no mycellium. [Re: botanisthype]
    #1851301 - 08/26/03 02:14 PM (13 years, 4 months ago)

Yeah im doing karo with the syringes that come


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OfflinePsilocybeingzz
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Re: Day 6, no mycellium. [Re: Sam_Kohui]
    #1851314 - 08/26/03 02:20 PM (13 years, 4 months ago)

I have had jars that I didnt see growth after 2 weeks , but after waaiting and waiting they start (to show)


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OfflineTantalus
Beyonddescription.
Registered: 06/05/03
Posts: 747
Last seen: 11 years, 4 months
Re: Day 6, no mycellium. [Re: Psilocybeingzz]
    #1851643 - 08/26/03 04:13 PM (13 years, 4 months ago)

Do you have a link to where the 1/2 & 1/2 was recommended? I've never seen that & would like to see the logic behind it.


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"A nation's hope of lasting peace cannot be firmly based upon any race in armaments but rather upon just relations and honest understanding with all other nations...

Every gun that is made, every warship launched, every rocket fired signifies, in the final sense, a theft from those who hunger and are not fed, those who are cold and are not clothed... The cost of one modern heavy bomber is this: a modern brick school in more than 30 cities. We pay for a single fighter with a half million bushels of wheat. We pay for a single destroyer with new homes that could have housed more than 8,000 people..."

President Dwight Eisenhower, 1953


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OfflineRohypnol
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Registered: 08/04/03
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Re: Day 6, no mycellium. [Re: Tantalus]
    #1851931 - 08/26/03 05:48 PM (13 years, 4 months ago)

Sorry I didn't mean 1/2 1/2 I meant 2:1 :smile:


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Invisiblebotanisthype
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Re: Day 6, no mycellium. [Re: Rohypnol]
    #1851958 - 08/26/03 05:56 PM (13 years, 4 months ago)

lol


--------------------
Please +REP if i've been of any help... thanks!!!

Dinosaur of the Funny Family, though I have short arms, they fit in my GB... lol


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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