NineInchNails said:

Everyone above IS giving you good info believe it or not.

1. is this all to do: open the plastic box, put it in the bag and close the bag?
You can inoculate a bag many different ways. If you use a syringe then you can do it out in the open air, just use sterile technique. Inject through the bag/inoculation point.

You can make a liquid culture and inoculate the bag in the open air using a syringe. Just use sterile technique. Inject through the bag/inoculation point.

You can open the bag inside of a glove box, but you'll need some MYC to drop in there.


2. should i dunk it ? probably not worth while.
I can't imagine why you would dunk a bag of colonized substrate. What do you plan to do with it? Do you plan on spawning it to a bulk substrate?

3. can i take samples from the mycelium to propagate in agar and LC?
Absolutely. Any growth will inoculate anything.

4. if so should i cut from the corner?
Cut from the corner of what?

5. will this stunt the mushroom fruiting?
Are you asking if grabbing a hunk of MYC from a fully colonized bag of substrate will harm fruiting? No, it wouldn't, but you should still do this inside of a glove box. You want to make sure that you do not contaminate what you are trying to inoculate.

6. will it make it prone to infection?
Grabbing a hunk of MYC from a fully colonized bag of substrate out in the open air and dropping onto some agar would likely contam the agar. Anything you inoculate by opening sterile containers should be done in at least a glove box.

---

DrWhite said:

No No to me that sounds like a casing kit. you take your stuff from your bag and put it in the Box. Maybe if you took a picture of your kit people could be more helpful.

---